Rabbit Polyclonal MCM4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Xenopus laevis samples. Cited in 31 publications. Immunogen corresponding to Synthetic Peptide within Human MCM4 aa 750-800.
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Publications
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- Nature communications 14:80492023RIF1 regulates early replication timing in murine B cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel Malzl,Mihaela Peycheva,Ali Rahjouei,Stefano Gnan,Kyle N Klein,Mariia Nazarova,Ursula E Schoeberl,David M Gilbert,Sara C B Buonomo,Michela Di Virgilio,Tobias Neumann,Rushad PavriPubMed 38081811
- Nature 606:197-2032022MCM complexes are barriers that restrict cohesin-mediated loop extrusion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBart J H Dequeker,Matthias J Scherr,Hugo B Brandão,Johanna Gassler,Sean Powell,Imre Gaspar,Ilya M Flyamer,Aleksandar Lalic,Wen Tang,Roman Stocsits,Iain F Davidson,Jan-Michael Peters,Karl E Duderstadt,Leonid A Mirny,Kikuë TachibanaPubMed 35585235
- Life science alliance 5:2022Mre11 exonuclease activity promotes irreversible mitotic progression under replication stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoshitami Hashimoto,Hirofumi TanakaPubMed 35292537
- Current protocols 1:e2192021Targeted Protein Depletion Using the Auxin-Inducible Degron 2 (AID2) System.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuichiro Saito,Masato T KanemakiPubMed 34370399
- Scientific reports 11:37612021Arsenic hexoxide has differential effects on cell proliferation and genome-wide gene expression in human primary mammary epithelial and MCF7 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDonguk Kim,Na Yeon Park,Keunsoo Kang,Stuart K Calderwood,Dong-Hyung Cho,Ill Ju Bae,Heeyoun BunchPubMed 33580144
- OncoTargets and therapy 13:12015-120252020Huaier Suppresses the Hepatocellular Carcinoma Cell Cycle by Regulating Minichromosome Maintenance Proteins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYongjie Niu et. al.PubMed 33244243
- The Journal of biological chemistry :2020Ongoing replication forks delay nuclear envelope breakdown upon mitotic entry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoshitami Hashimoto,Hirofumi TanakaPubMed 33148697
- BioMed research international 2020:35742612020Identification of the DNA Replication Regulator MCM Complex Expression and Prognostic Significance in Hepatic Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing Cao et. al.PubMed 32964028
- Cell reports 32:1080962020ATR Restrains DNA Synthesis and Mitotic Catastrophe in Response to CDC7 Inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael D Rainey,Declan Bennett,Rachel O'Dea,Melania E Zanchetta,Muriel Voisin,Cathal Seoighe,Corrado SantocanalePubMed 32877678
- Oncogene 39:5124-51372020Histone chaperone FACT is essential to overcome replication stress in mammalian cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura Prendergast et. al.PubMed 32533099
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MCM4 aa 750-800. The exact immunogen used to generate this antibody is proprietary information. Database link P33991
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Tested |
| Mouse | Tested | Not recommended | Expected | Expected |
| Chimpanzee | Predicted | Not recommended | Predicted | Predicted |
| Cow | Predicted | Not recommended | Predicted | Predicted |
| Drosophila melanogaster | Predicted | Not recommended | Predicted | Predicted |
| Elephant | Predicted | Not recommended | Predicted | Predicted |
| Gorilla | Predicted | Not recommended | Predicted | Predicted |
| Guinea pig | Predicted | Not recommended | Predicted | Predicted |
| Horse | Predicted | Not recommended | Predicted | Predicted |
| Opossum | Predicted | Not recommended | Predicted | Predicted |
| Orangutan | Predicted | Not recommended | Predicted | Predicted |
| Rabbit | Predicted | Not recommended | Predicted | Predicted |
| Rhesus monkey | Predicted | Not recommended | Predicted | Predicted |
| Xenopus laevis | Expected | Not recommended | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Xenopus laevis | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Orangutan, Rabbit, Opossum, Horse, Elephant, Drosophila melanogaster, Rhesus monkey, Chimpanzee, Gorilla, Guinea pig | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Xenopus laevis, Human, Cow, Orangutan, Rabbit, Opossum, Horse, Elephant, Drosophila melanogaster, Rhesus monkey, Chimpanzee, Gorilla, Guinea pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Xenopus laevis | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Orangutan, Rabbit, Opossum, Horse, Elephant, Drosophila melanogaster, Rhesus monkey, Chimpanzee, Gorilla, Guinea pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Species Xenopus laevis | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Orangutan, Rabbit, Opossum, Horse, Elephant, Drosophila melanogaster, Rhesus monkey, Chimpanzee, Gorilla, Guinea pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:16899510, PubMed:25661590, PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232, PubMed:9305914). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:16899510, PubMed:25661590, PubMed:32453425, PubMed:9305914).
Alternative names
CDC21, MCM4, DNA replication licensing factor MCM4, CDC21 homolog, P1-CDC21
Recommended products
Rabbit Polyclonal MCM4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Xenopus laevis samples. Cited in 31 publications. Immunogen corresponding to Synthetic Peptide within Human MCM4 aa 750-800.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MCM4 aa 750-800. The exact immunogen used to generate this antibody is proprietary information. Database link P33991
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MCM4 or Minichromosome Maintenance Complex Component 4 is a part of the MCM2-7 complex which is essential for the initiation and progression of DNA replication. It has a molecular mass of approximately 95 kDa. MCM4 functions as a DNA helicase unwinding DNA strands to allow replication forks to progress. This protein is expressed in various tissues with higher levels detected in dividing cells indicating its role in cell proliferation.
- Biological function summary
MCM4 is a critical player in cell cycle regulation as it participates in the formation of the pre-replicative complex during the G1 phase of the cell cycle. Its function becomes evident as it interacts within the MCM2-7 hexameric complex important for helicase activity and stability. This complex prevents DNA re-replication within a single cell cycle ensuring the maintenance of genomic integrity.
- Pathways
Several cellular mechanisms engage MCM4 particularly the DNA replication and cell cycle pathways. MCM4 associates with proteins like CDC45 and GINS within the CMG complex facilitating the activation and elongation phases of DNA replication. This relationship places MCM4 at the heart of orchestrating proper DNA synthesis and mitotic entry.
- Associated diseases and disorders
Researchers have linked MCM4 abnormalities to cancer progression and genomic instability. Overexpression or mutations in MCM4 disrupts normal replication processes heightening the risk for various cancers including breast and ovarian cancer. It also demonstrates interaction with the tumor suppressor protein p53 suggesting its involvement in processes that underpin malignancies linked to failed checkpoint control.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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5 product images
Western blot - Anti-MCM4 antibody (ab4459)
HeLa cell RIPA extract incubated with 0.2 μg/ml of ab4459 for 1 hour. HeLa cell RIPA extract incubated with 0.2 µg/ml of ab4459 for 1 hour.
All lanes: Western blot - Anti-MCM4 antibody (ab4459)
Predicted band size: 97 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab4459)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma (left) and mouse squamous cell carcinoma (right) tissues labelling MCM4 with ab4459 at 1/1000 (1µg/ml). Detection: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (ab4459)
IHC image of MCM4 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4459, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Anti-MCM4 antibody (ab4459)
This image is an edited version of an image received courtesy of an Abreview submitted by Jason DeHart on 22 August 2005. We do not have any further information relating to this image.
All lanes: Western blot - Anti-MCM4 antibody (ab4459) at 1/2000 dilution
All lanes: HeLa nuclear cell lysate. at 20 µg
SecondaryAll lanes: Goat anti rabbit HRP. at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 97 kDa
Immunocytochemistry/ Immunofluorescence - Anti-MCM4 antibody (ab4459)
ICC/IF image of ab4459 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4459, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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