AB124836

Anti-MCM4 antibody [EPR5245]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal MCM4 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

View Alternative Names

CDC21, MCM4, DNA replication licensing factor MCM4, CDC21 homolog, P1-CDC21

3 Images

Flow Cytometry (Intracellular) - Anti-MCM4 antibody [EPR5245] (AB124836)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-MCM4 antibody [EPR5245] (AB124836)

Intracellular flow cytometric analysis of permeabilized Molt-4 cells using anti-MCM4 in red (ab124836) or a rabbit IgG in green (negative).

Unknown

Western blot - Anti-MCM4 antibody [EPR5245] (AB124836)

All lanes:

Western blot - Anti-MCM4 antibody [EPR5245] (ab124836) at 1/50000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Molt-4 cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Predicted band size: 97 kDa

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OI-RD Scanning - Anti-MCM4 antibody [EPR5245] (AB124836)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-MCM4 antibody [EPR5245] (AB124836)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-MCM4 antibody [EPR5245] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5245

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000 - 1/200000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCM4 or Minichromosome Maintenance Complex Component 4 is a part of the MCM2-7 complex which is essential for the initiation and progression of DNA replication. It has a molecular mass of approximately 95 kDa. MCM4 functions as a DNA helicase unwinding DNA strands to allow replication forks to progress. This protein is expressed in various tissues with higher levels detected in dividing cells indicating its role in cell proliferation.

Biological function summary

MCM4 is a critical player in cell cycle regulation as it participates in the formation of the pre-replicative complex during the G1 phase of the cell cycle. Its function becomes evident as it interacts within the MCM2-7 hexameric complex important for helicase activity and stability. This complex prevents DNA re-replication within a single cell cycle ensuring the maintenance of genomic integrity.

Pathways

Several cellular mechanisms engage MCM4 particularly the DNA replication and cell cycle pathways. MCM4 associates with proteins like CDC45 and GINS within the CMG complex facilitating the activation and elongation phases of DNA replication. This relationship places MCM4 at the heart of orchestrating proper DNA synthesis and mitotic entry.

Researchers have linked MCM4 abnormalities to cancer progression and genomic instability. Overexpression or mutations in MCM4 disrupts normal replication processes heightening the risk for various cancers including breast and ovarian cancer. It also demonstrates interaction with the tumor suppressor protein p53 suggesting its involvement in processes that underpin malignancies linked to failed checkpoint control.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed : 16899510, PubMed : 25661590, PubMed : 32453425, PubMed : 34694004, PubMed : 34700328, PubMed : 35585232, PubMed : 9305914). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed : 16899510, PubMed : 25661590, PubMed : 32453425, PubMed : 9305914).

See full target information MCM4

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of cardiothoracic surgery 20:244 PubMed40437556

2025

Transcription factor E2F1 promotes non-small cell lung cancer progression by activating the PI3K/AKT pathway through MCM4.

Applications

Unspecified application

Species

Unspecified reactive species

Yuyin Cai,Xinyan Lu,Tingting Li,Jia Liu,Lifeng Jiang

Glia 70:2290-2308 PubMed35912412

2022

Novel potent liposome agonists of triggering receptor expressed on myeloid cells 2 phenocopy antibody treatment in cells.

Applications

Unspecified application

Species

Unspecified reactive species

Christophe Boudesco,Annelies Nonneman,Alessandro Cinti,Paola Picardi,Loredana Redaelli,Sofie Swijsen,Julian Roewe,Peter Reinhardt,Melanie Ibach,Jochen Walter,Jennifer M Pocock,Yi Ren,Pierre-Alexandre Driguez,Gihad Dargazanli,Stephanie Eyquem,Jonathan Proto,Amilcar Flores-Morales,Laurent Pradier

Pharmaceuticals (Basel, Switzerland) 15: PubMed35337060

2022

A Combined Treatment with Berberine and Andrographis Exhibits Enhanced Anti-Cancer Activity through Suppression of DNA Replication in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yinghui Zhao,Souvick Roy,Chuanxin Wang,Ajay Goel

Journal of cellular biochemistry 120:9506-9513 PubMed30520128

2018

Quantitative proteomics using SILAC-MS identifies N-acetylcysteine-solution-triggered reversal response of renal cell carcinoma cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Cuihong Zhao,Jianhua Chen,Yong Ling,Shenghai Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MCM4 antibody [EPR5245]

Flow Cytometry (Intracellular) - Anti-MCM4 antibody [EPR5245] (AB124836)

Western blot - Anti-MCM4 antibody [EPR5245] (AB124836)

OI-RD Scanning - Anti-MCM4 antibody [EPR5245] (AB124836)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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