Rabbit Polyclonal MCM5 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 28 publications. Immunogen corresponding to Synthetic Peptide within Human MCM5.
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- Nature communications 14:51472023RNAPII-dependent ATM signaling at collisions with replication forks.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElias Einig,Chao Jin,Valentina Andrioletti,Boris Macek,Nikita PopovPubMed 37620345
- iScience 26:1059742023Risk model based on minichromosome maintenance 2 using objective assessment for predicting survival of neuroblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang Zeng,Xiao-Yun Liu,Lei Miao,Kai Chen,Hui Xu,Liang-Jun Qin,Meng Li,Kai Liu,Jiahao Feng,Hai-Yun WangPubMed 36756367
- Nature communications 13:60902022Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHana Polasek-Sedlackova,Thomas C R Miller,Jana Krejci,Maj-Britt Rask,Jiri LukasPubMed 36241664
- Signal transduction and targeted therapy 7:1022022LSD1 is required for euchromatic origin firing and replication timing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYue Wang,Yunchao Huang,Edith Cheng,Xinhua Liu,Yu Zhang,Jianguo Yang,Jordan T F Young,Grant W Brown,Xiaohan Yang,Yongfeng ShangPubMed 35414135
- Pharmaceuticals (Basel, Switzerland) 15:2022A Combined Treatment with Berberine and Andrographis Exhibits Enhanced Anti-Cancer Activity through Suppression of DNA Replication in Colorectal Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYinghui Zhao,Souvick Roy,Chuanxin Wang,Ajay GoelPubMed 35337060
- Life science alliance 5:2022Refining the domain architecture model of the replication origin firing factor Treslin/TICRR.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPedro Ferreira,Luis Sanchez-Pulido,Anika Marko,Chris P Ponting,Dominik BoosPubMed 35091422
- Communications biology 4:7672021The γ-tubulin meshwork assists in the recruitment of PCNA to chromatin in mammalian cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthieu Corvaisier,Jingkai Zhou,Darina Malycheva,Nicola Cornella,Dimitrios Chioureas,Nina M S Gustafsson,Catalina Ana Rosselló,Silvia Ayora,Tongbin Li,Kristina Ekström-Holka,Karin Jirström,Lisa Lindström,Maria Alvarado-KristenssonPubMed 34158617
- Clinical epigenetics 13:372021BRG1 knockdown inhibits proliferation through multiple cellular pathways in prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatherine A Giles,Cathryn M Gould,Joanna Achinger-Kawecka,Scott G Page,Georgia R Kafer,Samuel Rogers,Phuc-Loi Luu,Anthony J Cesare,Susan J Clark,Phillippa C TaberlayPubMed 33596994
- Nature communications 11:39512020DONSON and FANCM associate with different replisomes distinguished by replication timing and chromatin domain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Zhang et. al.PubMed 32769987
- Nature 574:571-5742019Stabilization of chromatin topology safeguards genome integrity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFena Ochs et. al.PubMed 31645724
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human MCM5. The exact immunogen used to generate this antibody is proprietary information. Database link P33992
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Chimpanzee | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000.00000 - 1/20000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:16899510, PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:32453425).
Alternative names
CDC46, MCM5, DNA replication licensing factor MCM5, CDC46 homolog, P1-CDC46
Recommended products
Rabbit Polyclonal MCM5 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 28 publications. Immunogen corresponding to Synthetic Peptide within Human MCM5.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human MCM5. The exact immunogen used to generate this antibody is proprietary information. Database link P33992
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein MCM5 also known as Minichromosome Maintenance Complex Component 5 plays a significant role in DNA replication. It is a part of the minichromosome maintenance (MCM) family which consists of several proteins forming an important component of the pre-replication complex. MCM5 has a molecular mass of approximately 82 kDa. This protein is ubiquitously expressed in cells underlining its fundamental role in the cell cycle. The presence of MCM5 ensures the fidelity and timing of DNA replication initiation during the S phase of cell division.
- Biological function summary
As a component of the MCM2-7 hexameric complex MCM5 contributes to the unwinding of DNA an essential step for replication. This complex acts as a helicase unwinding the DNA double helix at replication origins. This activity is required for laying down the replication fork and allowing access for the DNA polymerase. MCM5 integrates into the larger assembly of the replication machinery working in concert with cell cycle regulators to ensure proper cell cycle progression.
- Pathways
MCM5 participates in DNA replication and cell cycle control pathways. It closely associates with the GINS complex and CDC45 forming the CMG (CDC45-MCM-GINS) complex which is critical for replication fork progression. MCM5 also interacts with the ATR-mediated DNA damage repair pathway. This relationship provides a checkpoint mechanism to halt replication in response to DNA damage allowing time for repair processes to maintain genomic stability.
- Associated diseases and disorders
MCM5 has implications in cancer and replication stress-related conditions. Overexpression or dysregulation of MCM5 can lead to uncontrolled cell proliferation contributing to oncogenesis. MCM5 has a notable connection to cyclin D1 and the Retinoblastoma (Rb) protein pathway often disrupted in cancers. Moreover in diseases characterized by replication stress such as certain neurodegenerative disorders improper MCM5 function can exacerbate genomic instability leading to disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-MCM5 antibody (ab17967)
All lanes: Western blot - Anti-MCM5 antibody (ab17967) at 0.05 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: Jurkat whole cell lysate at 50 µg
Predicted band size: 82 kDa
Exposure time: 10s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody (ab17967)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling MCM5 with ab17967 at 1/1000 (1µg/ml). Detection: DAB.
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