Mouse Monoclonal MCM5 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MCM5.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [CRCT5.1 (A2.7A3)] (AB6154), expandable thumbnail](https://content.abcam.com/products/images/mcm5-antibody-crct51-a27a3-ab6154--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103751.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [CRCT5.1 (A2.7A3)] (AB6154)")
Publications
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- Cancer cell international 19:2822019CDK5RAP3 inhibits angiogenesis in gastric neuroendocrine carcinoma by modulating AKT/HIF-1α/VEGFA signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJian-Xian Lin et. al.PubMed 31728130
- Cell 174:803-817.e162018Cross-talk between Lysine-Modifying Enzymes Controls Site-Specific DNA Amplifications.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSweta Mishra et. al.PubMed 30057114
- Scientific reports 6:308192016Probing the Spatial Organization of Molecular Complexes Using Triple-Pair-Correlation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYandong Yin et. al.PubMed 27545293
- Journal of virology 89:917-302014Interactome analysis of the human respiratory syncytial virus RNA polymerase complex identifies protein chaperones as important cofactors that promote L-protein stability and RNA synthesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDiane C Munday,Weining Wu,Nikki Smith,Jenna Fix,Sarah Louise Noton,Marie Galloux,Olivier Touzelet,Stuart D Armstrong,Jenna M Dawson,Waleed Aljabr,Andrew J Easton,Marie-Anne Rameix-Welti,Andressa Peres de Oliveira,Fernando M Simabuco,Armando M Ventura,David J Hughes,John N Barr,Rachel Fearns,Paul Digard,Jean-François Eléouët,Julian A HiscoxPubMed 25355874
- The Journal of biological chemistry 283:28729-402008Hepatitis B virus X protein increases the Cdt1-to-geminin ratio inducing DNA re-replication and polyploidy.
- Proceedings of the National Academy of Sciences of the United States of America 95:14932-71998Improved cervical smear assessment using antibodies against proteins that regulate DNA replication.Applications:Unspecified applicationReactive species:Unspecified reactive speciesG H Williams,P Romanowski,L Morris,M Madine,A D Mills,K Stoeber,J Marr,R A Laskey,N ColemanPubMed 9843993
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
Preservative: 0.1% Sodium azide
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human MCM5. Database link P33992
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/25.00000 - 1/100.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Associated Products
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Target data
Function
Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:16899510, PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:32453425).
Alternative names
CDC46, MCM5, DNA replication licensing factor MCM5, CDC46 homolog, P1-CDC46
Recommended products
Mouse Monoclonal MCM5 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MCM5.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
Preservative: 0.1% Sodium azide
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human MCM5. Database link P33992
- Clone number
- CRCT5.1 (A2.7A3)
- Purification technique
- Affinity purification Protein G
- Specificity
Recognises human Mcm-5
- Concentration
- Purification notes
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein MCM5 also known as Minichromosome Maintenance Complex Component 5 plays a significant role in DNA replication. It is a part of the minichromosome maintenance (MCM) family which consists of several proteins forming an important component of the pre-replication complex. MCM5 has a molecular mass of approximately 82 kDa. This protein is ubiquitously expressed in cells underlining its fundamental role in the cell cycle. The presence of MCM5 ensures the fidelity and timing of DNA replication initiation during the S phase of cell division.
- Biological function summary
As a component of the MCM2-7 hexameric complex MCM5 contributes to the unwinding of DNA an essential step for replication. This complex acts as a helicase unwinding the DNA double helix at replication origins. This activity is required for laying down the replication fork and allowing access for the DNA polymerase. MCM5 integrates into the larger assembly of the replication machinery working in concert with cell cycle regulators to ensure proper cell cycle progression.
- Pathways
MCM5 participates in DNA replication and cell cycle control pathways. It closely associates with the GINS complex and CDC45 forming the CMG (CDC45-MCM-GINS) complex which is critical for replication fork progression. MCM5 also interacts with the ATR-mediated DNA damage repair pathway. This relationship provides a checkpoint mechanism to halt replication in response to DNA damage allowing time for repair processes to maintain genomic stability.
- Associated diseases and disorders
MCM5 has implications in cancer and replication stress-related conditions. Overexpression or dysregulation of MCM5 can lead to uncontrolled cell proliferation contributing to oncogenesis. MCM5 has a notable connection to cyclin D1 and the Retinoblastoma (Rb) protein pathway often disrupted in cancers. Moreover in diseases characterized by replication stress such as certain neurodegenerative disorders improper MCM5 function can exacerbate genomic instability leading to disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [CRCT5.1 (A2.7A3)] (ab6154), expandable thumbnail](https://content.abcam.com/products/images/mcm5-antibody-crct51-a27a3-ab6154--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103751.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [CRCT5.1 (A2.7A3)] (ab6154)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [CRCT5.1 (A2.7A3)] (ab6154)
ab6154 staining MCM5 in human tonsil by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
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