AB6154

Anti-MCM5 antibody [CRCT5.1 (A2.7A3)]

Name: Anti-MCM5 antibody [CRCT5.1 (A2.7A3)] (ab6154) | Abcam
Brand: Abcam Limited
SKU: AB6154
Rating: 5 (1 reviews)

(1 Review)

(6 Publications)

Mouse Monoclonal MCM5 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MCM5.

View Alternative Names

CDC46, MCM5, DNA replication licensing factor MCM5, CDC46 homolog, P1-CDC46

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [CRCT5.1 (A2.7A3)] (AB6154)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [CRCT5.1 (A2.7A3)] (AB6154)

ab6154 staining MCM5 in human tonsil by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CRCT5.1 (A2.7A3)

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MCM5.

P33992

Specificity

Recognises human Mcm-5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Storage buffer

Preservative: 0.1% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein MCM5 also known as Minichromosome Maintenance Complex Component 5 plays a significant role in DNA replication. It is a part of the minichromosome maintenance (MCM) family which consists of several proteins forming an important component of the pre-replication complex. MCM5 has a molecular mass of approximately 82 kDa. This protein is ubiquitously expressed in cells underlining its fundamental role in the cell cycle. The presence of MCM5 ensures the fidelity and timing of DNA replication initiation during the S phase of cell division.

Biological function summary

As a component of the MCM2-7 hexameric complex MCM5 contributes to the unwinding of DNA an essential step for replication. This complex acts as a helicase unwinding the DNA double helix at replication origins. This activity is required for laying down the replication fork and allowing access for the DNA polymerase. MCM5 integrates into the larger assembly of the replication machinery working in concert with cell cycle regulators to ensure proper cell cycle progression.

Pathways

MCM5 participates in DNA replication and cell cycle control pathways. It closely associates with the GINS complex and CDC45 forming the CMG (CDC45-MCM-GINS) complex which is critical for replication fork progression. MCM5 also interacts with the ATR-mediated DNA damage repair pathway. This relationship provides a checkpoint mechanism to halt replication in response to DNA damage allowing time for repair processes to maintain genomic stability.

MCM5 has implications in cancer and replication stress-related conditions. Overexpression or dysregulation of MCM5 can lead to uncontrolled cell proliferation contributing to oncogenesis. MCM5 has a notable connection to cyclin D1 and the Retinoblastoma (Rb) protein pathway often disrupted in cancers. Moreover in diseases characterized by replication stress such as certain neurodegenerative disorders improper MCM5 function can exacerbate genomic instability leading to disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed : 16899510, PubMed : 32453425, PubMed : 34694004, PubMed : 34700328, PubMed : 35585232). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed : 32453425).

See full target information MCM5

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 19:282 PubMed31728130

2019

CDK5RAP3 inhibits angiogenesis in gastric neuroendocrine carcinoma by modulating AKT/HIF-1α/VEGFA signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Xian Lin,Xiong-Feng Weng,Xin-Sheng Xie,Ning-Zi Lian,Sheng-Liang Qiu,Jia-Bin Wang,Jun Lu,Qi-Yue Chen,Long-Long Cao,Mi Lin,Ru-Hong Tu,Ying-Hong Yang,Si-Jia Liu,Min Hu,Yi-Ke Lin,Chang-Ming Huang,Chao-Hui Zheng,Ping Li,Jian-Wei Xie

Cell 174:803-817.e16 PubMed30057114

2018

Cross-talk between Lysine-Modifying Enzymes Controls Site-Specific DNA Amplifications.

Applications

Unspecified application

Species

Unspecified reactive species

Sweta Mishra,Capucine Van Rechem,Sangita Pal,Thomas L Clarke,Damayanti Chakraborty,Sarah D Mahan,Joshua C Black,Sedona E Murphy,Michael S Lawrence,Danette L Daniels,Johnathan R Whetstine

Scientific reports 6:30819 PubMed27545293

2016

Probing the Spatial Organization of Molecular Complexes Using Triple-Pair-Correlation.

Applications

Unspecified application

Species

Unspecified reactive species

Yandong Yin,Eli Rothenberg

Journal of virology 89:917-30 PubMed25355874

2014

Interactome analysis of the human respiratory syncytial virus RNA polymerase complex identifies protein chaperones as important cofactors that promote L-protein stability and RNA synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Diane C Munday,Weining Wu,Nikki Smith,Jenna Fix,Sarah Louise Noton,Marie Galloux,Olivier Touzelet,Stuart D Armstrong,Jenna M Dawson,Waleed Aljabr,Andrew J Easton,Marie-Anne Rameix-Welti,Andressa Peres de Oliveira,Fernando M Simabuco,Armando M Ventura,David J Hughes,John N Barr,Rachel Fearns,Paul Digard,Jean-François Eléouët,Julian A Hiscox

The Journal of biological chemistry 283:28729-40 PubMed18693245

2008

Hepatitis B virus X protein increases the Cdt1-to-geminin ratio inducing DNA re-replication and polyploidy.

Applications

ICC/IF

Species

Mouse

Lova Rakotomalala,Leo Studach,Wen-Horng Wang,Gerald Gregori,Ronald L Hullinger,Ourania Andrisani

Proceedings of the National Academy of Sciences of the United States of America 95:14932-7 PubMed9843993

1998

Improved cervical smear assessment using antibodies against proteins that regulate DNA replication.

Applications

Unspecified application

Species

Unspecified reactive species

G H Williams,P Romanowski,L Morris,M Madine,A D Mills,K Stoeber,J Marr,R A Laskey,N Coleman

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