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Rabbit Monoclonal MCM5 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.


Images

Western blot - Anti-MCM5 antibody [EP2682Y] (AB76023), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (AB76023), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (AB76023), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (AB76023), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (AB76023), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/100.00000 - 1/250.00000
Notes

Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/1000.00000 - 1/2000.00000
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:16899510, PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:32453425).

Alternative names

CDC46, MCM5, DNA replication licensing factor MCM5, CDC46 homolog, P1-CDC46

Recommended products

Rabbit Monoclonal MCM5 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP2682Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein MCM5 also known as Minichromosome Maintenance Complex Component 5 plays a significant role in DNA replication. It is a part of the minichromosome maintenance (MCM) family which consists of several proteins forming an important component of the pre-replication complex. MCM5 has a molecular mass of approximately 82 kDa. This protein is ubiquitously expressed in cells underlining its fundamental role in the cell cycle. The presence of MCM5 ensures the fidelity and timing of DNA replication initiation during the S phase of cell division.

Biological function summary

As a component of the MCM2-7 hexameric complex MCM5 contributes to the unwinding of DNA an essential step for replication. This complex acts as a helicase unwinding the DNA double helix at replication origins. This activity is required for laying down the replication fork and allowing access for the DNA polymerase. MCM5 integrates into the larger assembly of the replication machinery working in concert with cell cycle regulators to ensure proper cell cycle progression.

Pathways

MCM5 participates in DNA replication and cell cycle control pathways. It closely associates with the GINS complex and CDC45 forming the CMG (CDC45-MCM-GINS) complex which is critical for replication fork progression. MCM5 also interacts with the ATR-mediated DNA damage repair pathway. This relationship provides a checkpoint mechanism to halt replication in response to DNA damage allowing time for repair processes to maintain genomic stability.

Associated diseases and disorders

MCM5 has implications in cancer and replication stress-related conditions. Overexpression or dysregulation of MCM5 can lead to uncontrolled cell proliferation contributing to oncogenesis. MCM5 has a notable connection to cyclin D1 and the Retinoblastoma (Rb) protein pathway often disrupted in cancers. Moreover in diseases characterized by replication stress such as certain neurodegenerative disorders improper MCM5 function can exacerbate genomic instability leading to disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-MCM5 antibody [EP2682Y] (ab76023), expandable thumbnail

    Western blot - Anti-MCM5 antibody [EP2682Y] (ab76023)

    All lanes: Western blot - Anti-MCM5 antibody [EP2682Y] (ab76023) at 1/2000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: MCF7 cell lysate at 10 µg

    Lane 3: Human spleen lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 82 kDa

    Observed band size: 82 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)

    ab76023, at a 1/100 dilution, staining MCM5 in paraffin embedded human squamous cell cervical carcinoma tissue. A HRP/AP polymerized secondary antibody was used.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)

    ab76023 showing positive staining in Normal tonsil tissue.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)

    ab76023 showing negative staining in Normal brain tissue.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)

    ab76023 showing positive staining in Glioma tissue.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)

    ab76023 showing positive staining in Colonic adenocarcinoma tissue.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)

    ab76023 showing positive staining in Endometrial carcinoma tissue.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM5 antibody [EP2682Y] (ab76023)

    ab76023 showing positive staining in Ovarian carcinoma tissue.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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