Skip to main content

Mouse Recombinant Monoclonal MCM6 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-MCM6 antibody [1/MCM6] (AB280208), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [1/MCM6] (AB280208), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [1/MCM6] (AB280208), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MCM6 antibody [1/MCM6] (AB280208), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MCM6 antibody [1/MCM6] (AB280208), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/200
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/20
Notes

-

Target data

Function

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:16899510, PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232, PubMed:9305914). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:32453425).

Alternative names

DNA replication licensing factor MCM6, p105MCM, MCM6

Recommended products

Mouse Recombinant Monoclonal MCM6 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Clone number
1/MCM6
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The target known as MCM6 or Minichromosome Maintenance Complex Component 6 is a part of a group of proteins providing essential functions during DNA replication initiation. MCM6 is a part of the hexameric MCM2-7 complex which operates as a helicase that unwinds DNA to facilitate fork progression. This protein has an approximate molecular weight of 92 kDa. MCM6 expresses in dividing cells across different tissues particularly in cells with high proliferative rates like those in the bone marrow and gastrointestinal tract.

Biological function summary

The functions of MCM6 contribute to the precise control of DNA replication and overall cell cycle regulation. The protein forms a part of the MCM2-7 complex playing a significant role in the S phase to ensure DNA replication occurs at the right time. Additionally the MCM complex acts as a primary licensing factor for replication origins preventing re-replication. MCM6 also interacts with other proteins like CDC45 and GINS to form the CMG complex a critical component for replication fork stability and duplication.

Pathways

MCM6 contributes significantly within the DNA replication and cell cycle pathways. As a component of the MCM2-7 complex it supports the unwinding of DNA which is a prerequisite for replication to initiate. MCM6 is related to proteins like CDC6 and ORC which are integral to the assembly of the pre-replication complex facilitating the initiation of DNA synthesis. This integration into core cellular mechanisms highlights its importance in genomic stability.

Associated diseases and disorders

Mutations or deregulation of MCM6 have links to cancerous growths and genomic instability disorders. The aberrant expression of MCM6 is often observed in cancers such as colorectal and breast cancer where it contributes to unchecked cellular proliferation. MCM6 correlates with oncogenic proteins like MYC which can amplify abnormal cell cycle progression. Understanding the mechanistic contributions of MCM6 in these conditions assists in developing therapeutic strategies targeting these pathways.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-MCM6 antibody [1/MCM6] (ab280208), expandable thumbnail

    Western blot - Anti-MCM6 antibody [1/MCM6] (ab280208)

    Blocking and Diluting buffer and concentration: 5% NFDM/TBST

    Exposure time lane 1-5: 37 seconds
    lane 6: 3 minutes

    All lanes: Western blot - Anti-MCM6 antibody [1/MCM6] (ab280208) at 1/1000 dilution

    Lane 1: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 2: HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate at 20 µg

    Lane 3: SW480 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 4: U-2 OS (human bone osteosarcoma epithelial cell), whole cell lysate at 20 µg

    Lane 5: A431 (human epidermoid carcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 6: Human tonsil tissue lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 93 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [1/MCM6] (ab280208), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [1/MCM6] (ab280208)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling MCM6 with ab280208 at 1/200 (4.945 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining in human colon.The section was incubated with ab280208 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [1/MCM6] (ab280208), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [1/MCM6] (ab280208)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MCM6 with ab280208 at 1/200 (4.945 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Nuclear staining in human tonsil.The section was incubated with ab280208 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunocytochemistry/ Immunofluorescence - Anti-MCM6 antibody [1/MCM6] (ab280208), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MCM6 antibody [1/MCM6] (ab280208)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A431 cells labelling MCM6 with ab280208 at 1/20 (49.45 ug/ml) dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in A431 cell line. Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MCM6 antibody [1/MCM6] (ab280208), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MCM6 antibody [1/MCM6] (ab280208)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized U-2 OS cells labelling MCM6 with ab280208 at 1/20 (49.45 ug/ml) dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in U-2 OS cell line. Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com