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AB327184

Anti-MCM6 Antibody

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Rabbit Polyclonal MCM6 antibody. Suitable for WB, IHC-P, ICC/IF, ChIP and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human MCM6.

View Alternative Names

DNA replication licensing factor MCM6, p105MCM, MCM6, Mcmd6, Mis5, DNA replication licensing factor MCM6, Mis5 homolog, Mcmd6, DNA replication licensing factor MCM6, Intestinal DNA replication protein

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 Antibody (AB327184)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 Antibody (AB327184)

Immunohistochemical staining of human liver using ab327184 at 1 : 50–1 : 200 dilution, showing low expression as expected.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 Antibody (AB327184)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 Antibody (AB327184)

Immunohistochemical staining of human lymph node using ab327184 at 1 : 50–1 : 200 dilution, showing high expression.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 Antibody (AB327184)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 Antibody (AB327184)

Immunohistochemistry analysis in human lymph node and liver tissues using ab327184 at 1 : 50–1 : 200 dilution. Corresponding MCM6 RNA-seq data are presented for the same tissues.

Immunocytochemistry/ Immunofluorescence - Anti-MCM6 Antibody (AB327184)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MCM6 Antibody (AB327184)

Immunofluorescent staining of human cell line U-2 OS using ab327184 at 0.25–2 µg/ml, showing localization to nucleoplasm.

ChIP - Anti-MCM6 Antibody (AB327184)
  • ChIP

Supplier Data

ChIP - Anti-MCM6 Antibody (AB327184)

ChIP-Exo-Seq composite graph for ab327184 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Western blot - Anti-MCM6 Antibody (AB327184)
  • WB

Supplier Data

Western blot - Anti-MCM6 Antibody (AB327184)

Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II using ab327184 at 0.04–0.4 µg/ml.

All lanes:

Western blot - Anti-MCM6 Antibody (ab327184)

Lane 2:

Mouse cell line NIH-3T3

Lane 3:

Rat cell line NBT-II

Predicted band size: 92.89 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, WB, IHC-P, ChIP

applications

Immunogen

Recombinant Fragment Protein within Human MCM6.

Q14566

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1-10 µg", "ChIP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed : 16899510, PubMed : 32453425, PubMed : 34694004, PubMed : 34700328, PubMed : 35585232, PubMed : 9305914). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed : 32453425).
See full target information MCM6

Additional targets

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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