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AB268083

Anti-MCM6 antibody [MCM6/2999]

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(1 Publication)

Mouse Monoclonal MCM6 antibody. Suitable for Protein Array, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MCM6 aa 200-400.

View Alternative Names

DNA replication licensing factor MCM6, p105MCM, MCM6

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [MCM6/2999] (AB268083)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [MCM6/2999] (AB268083)

Formalin-fixed, paraffin-embedded human colon tissue stained for MCM6 using ab268083 at 2 μg/ml in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [MCM6/2999] (AB268083)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [MCM6/2999] (AB268083)

Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for MCM6 using ab268083 at 2 μg/ml in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [MCM6/2999] (AB268083)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody [MCM6/2999] (AB268083)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for MCM6 using ab268083 at 2 μg/ml in immunohistochemical analysis.

Protein Array - Anti-MCM6 antibody [MCM6/2999] (AB268083)
  • Protein Array

Unknown

Protein Array - Anti-MCM6 antibody [MCM6/2999] (AB268083)

Protein Array containing >19,000 full-length human proteins using ab268083. Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MCM6/2999

Isotype

IgG2b

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

Protein Array, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MCM6 aa 200-400. The exact immunogen used to generate this antibody is proprietary information.

Q14566

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from Bioreactor concentrate
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target known as MCM6 or Minichromosome Maintenance Complex Component 6 is a part of a group of proteins providing essential functions during DNA replication initiation. MCM6 is a part of the hexameric MCM2-7 complex which operates as a helicase that unwinds DNA to facilitate fork progression. This protein has an approximate molecular weight of 92 kDa. MCM6 expresses in dividing cells across different tissues particularly in cells with high proliferative rates like those in the bone marrow and gastrointestinal tract.
Biological function summary

The functions of MCM6 contribute to the precise control of DNA replication and overall cell cycle regulation. The protein forms a part of the MCM2-7 complex playing a significant role in the S phase to ensure DNA replication occurs at the right time. Additionally the MCM complex acts as a primary licensing factor for replication origins preventing re-replication. MCM6 also interacts with other proteins like CDC45 and GINS to form the CMG complex a critical component for replication fork stability and duplication.

Pathways

MCM6 contributes significantly within the DNA replication and cell cycle pathways. As a component of the MCM2-7 complex it supports the unwinding of DNA which is a prerequisite for replication to initiate. MCM6 is related to proteins like CDC6 and ORC which are integral to the assembly of the pre-replication complex facilitating the initiation of DNA synthesis. This integration into core cellular mechanisms highlights its importance in genomic stability.

Mutations or deregulation of MCM6 have links to cancerous growths and genomic instability disorders. The aberrant expression of MCM6 is often observed in cancers such as colorectal and breast cancer where it contributes to unchecked cellular proliferation. MCM6 correlates with oncogenic proteins like MYC which can amplify abnormal cell cycle progression. Understanding the mechanistic contributions of MCM6 in these conditions assists in developing therapeutic strategies targeting these pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed : 16899510, PubMed : 32453425, PubMed : 34694004, PubMed : 34700328, PubMed : 35585232, PubMed : 9305914). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed : 32453425).
See full target information MCM6
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 9: PubMed34356903

2021

TGF-β1 Receptor Inhibitor SB525334 Attenuates the Epithelial to Mesenchymal Transition of Peritoneal Mesothelial Cells via the TGF-β1 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jung-Yoon Heo,Jun-Young Do,Yunmee Lho,A-Young Kim,Sang-Woon Kim,Seok-Hui Kang
View all publications
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