Rabbit Polyclonal MCM7/PRL antibody. Suitable for IP, ChIP, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DNA replication licensing factor MCM7 aa 1-300.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Liquid
Polyclonal
IP | ChIP | WB | IHC-P | ICC/IF | |
---|---|---|---|---|---|
Human | Tested | Tested | Tested | Tested | Tested |
Mouse | Predicted | Predicted | Predicted | Predicted | Predicted |
Rat | Predicted | Predicted | Predicted | Predicted | Predicted |
Cow | Predicted | Predicted | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/250.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Select an associated product type
Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:25661590, PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232, PubMed:9305914). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:32453425). Required for S-phase checkpoint activation upon UV-induced damage.
CDC47, MCM2, MCM7, CDC47, MCM2, DNA replication licensing factor MCM7, CDC47 homolog, P1.1-MCM3
Rabbit Polyclonal MCM7/PRL antibody. Suitable for IP, ChIP, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DNA replication licensing factor MCM7 aa 1-300.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
The MCM7 (Minichromosome Maintenance Complex Component 7) also known as PRL serves an important mechanical role in DNA replication. It is part of the MCM2-7 complex a helicase essential for the initiation and elongation phases of DNA replication. MCM7 has a molecular mass of approximately 81 kDa and is found expressed in the nucleus of proliferating cells. It is essential for unwinding the DNA helix preparing it for replication.
MCM7 is involved in maintaining genomic stability and ensuring accurate DNA replication. As part of the larger MCM2-7 hexameric complex it collaborates with other members to form the core component of the pre-replicative complex (pre-RC). By loading onto replication origins MCM7 helps license the DNA for duplication. This function contributes to cell cycle progression particularly the S phase where DNA synthesis occurs.
MCM7 plays an important role in DNA replication-related signaling pathways. It functions within the cell cycle and DNA replication pathways interacting closely with other essential proteins like CDC45 and GINS complex. These partnerships facilitate the transition from the G1 to the S phase triggering the replication machinery's activation and ensuring cell division processes continue smoothly.
MCM7 has been associated with various cancers including breast and prostate cancer. Aberrant expression or dysregulation of MCM7 contributes to uncontrolled cell proliferation linking it to tumorigenesis. Also MCM7 interacts with proteins such as cyclin D1 in certain oncogenic pathways emphasizing its role in the progression of cancerous conditions. Understanding these interactions offers insights into potential therapeutic targets for cancer treatment.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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7.5% SDS PAGE
All lanes: Western blot - Anti-MCM7/PRL antibody (ab96849) at 1/1000 dilution
All lanes: H1299 whole cell lysate at 30 µg
Predicted band size: 81 kDa
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using ab96849 at 1/200 dilution (1).Merged with DNA probe (2).
MCM7 was immunoprecipitated from 40 μg of HeLa whole cell lysate with ab96849 at 1/1000 dilution. EasyBlot anti-rabbit IgG, was used as secondary antibody.
Lane 1: 40 μg HeLa whole cell extract (Input).
Lane 2: Control with 4 μg of preimmune Rabbit IgG.
Lane 3: Immunoprecipitation of MCM7 protein by 4 μg MCM7 antibody [N2C2], Internal.
7.5 % SDS-PAGE
All lanes: Immunoprecipitation - Anti-MCM7/PRL antibody (ab96849)
Predicted band size: 81 kDa
Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-MCM7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft using ab96849 at 1/100 dilution.
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