AB96849

Anti-MCM7/PRL antibody

Name: Anti-MCM7/PRL antibody (ab96849) | Abcam
Brand: Abcam Limited
SKU: AB96849

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(2 Publications)

Rabbit Polyclonal MCM7/PRL antibody. Suitable for WB, IP, ChIP, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MCM7 aa 1 to C-terminus.

View Alternative Names

CDC47, MCM2, MCM7, DNA replication licensing factor MCM7, CDC47 homolog, P1.1-MCM3

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-MCM7/PRL antibody (AB96849)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MCM7/PRL antibody (AB96849)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using ab96849 at 1/200 dilution (1).Merged with DNA probe (2).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM7/PRL antibody (AB96849)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM7/PRL antibody (AB96849)

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft using ab96849 at 1/100 dilution.

Supplier Data

Immunoprecipitation - Anti-MCM7/PRL antibody (AB96849)

MCM7 was immunoprecipitated from 40 μg of HeLa whole cell lysate with ab96849 at 1/1000 dilution. EasyBlot anti-rabbit IgG, was used as secondary antibody.
Lane 1 : 40 μg HeLa whole cell extract (Input).
Lane 2 : Control with 4 μg of preimmune Rabbit IgG.
Lane 3 : Immunoprecipitation of MCM7 protein by 4 μg MCM7 antibody [N2C2], Internal.

7.5 % SDS-PAGE

All lanes:

Immunoprecipitation - Anti-MCM7/PRL antibody (ab96849)

Predicted band size: 81 kDa

false

ChIP

Supplier Data

ChIP - Anti-MCM7/PRL antibody (AB96849)

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-MCM7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.

Supplier Data

Western blot - Anti-MCM7/PRL antibody (AB96849)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-MCM7/PRL antibody (ab96849) at 1/1000 dilution

Lane 1:

A549 whole cell lysates at 30 µg

Lane 2:

H1299 whole cell lysates at 30 µg

Lane 3:

HCT116 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 81 kDa

false

Supplier Data

Western blot - Anti-MCM7/PRL antibody (AB96849)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-MCM7/PRL antibody (ab96849) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 81 kDa

false

Supplier Data

Western blot - Anti-MCM7/PRL antibody (AB96849)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-MCM7/PRL antibody (ab96849) at 1/1000 dilution

Lane 1:

NIH-3T3 whole cell lysates at 30 µg

Lane 2:

C6 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 81 kDa

false

Supplier Data

Western blot - Anti-MCM7/PRL antibody (AB96849)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-MCM7/PRL antibody (ab96849) at 1/1000 dilution

Lane 1:

Neuro2a whole cell lysates at 30 µg

Lane 2:

Rat2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 81 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ChIP, IP, WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human MCM7 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P33993

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MCM7 (Minichromosome Maintenance Complex Component 7) also known as PRL serves an important mechanical role in DNA replication. It is part of the MCM2-7 complex a helicase essential for the initiation and elongation phases of DNA replication. MCM7 has a molecular mass of approximately 81 kDa and is found expressed in the nucleus of proliferating cells. It is essential for unwinding the DNA helix preparing it for replication.

Biological function summary

MCM7 is involved in maintaining genomic stability and ensuring accurate DNA replication. As part of the larger MCM2-7 hexameric complex it collaborates with other members to form the core component of the pre-replicative complex (pre-RC). By loading onto replication origins MCM7 helps license the DNA for duplication. This function contributes to cell cycle progression particularly the S phase where DNA synthesis occurs.

Pathways

MCM7 plays an important role in DNA replication-related signaling pathways. It functions within the cell cycle and DNA replication pathways interacting closely with other essential proteins like CDC45 and GINS complex. These partnerships facilitate the transition from the G1 to the S phase triggering the replication machinery's activation and ensuring cell division processes continue smoothly.

MCM7 has been associated with various cancers including breast and prostate cancer. Aberrant expression or dysregulation of MCM7 contributes to uncontrolled cell proliferation linking it to tumorigenesis. Also MCM7 interacts with proteins such as cyclin D1 in certain oncogenic pathways emphasizing its role in the progression of cancerous conditions. Understanding these interactions offers insights into potential therapeutic targets for cancer treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed : 25661590, PubMed : 32453425, PubMed : 34694004, PubMed : 34700328, PubMed : 35585232, PubMed : 9305914). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed : 32453425). Required for S-phase checkpoint activation upon UV-induced damage.

See full target information MCM7

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Tohoku journal of experimental medicine : PubMed40738669

2025

Histone Lactylation Promotes Proliferation and Migration of TNBC Cells via Upregulating MCM7 Transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Xiao,Chaoxian Wang,Sili Jin,Ziran Zhang,Xingxia Yang,Guangfa Xia

Cell reports 7:575-587 PubMed24726359

2014

The RBBP6/ZBTB38/MCM10 axis regulates DNA replication and common fragile site stability.

Applications

Species

Unspecified reactive species

Benoit Miotto,Moredreck Chibi,Ping Xie,Stéphane Koundrioukoff,Hanlie Moolman-Smook,David Pugh,Michelle Debatisse,Fuchu He,Lingqiang Zhang,Pierre-Antoine Defossez

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MCM7/PRL antibody

Immunocytochemistry/ Immunofluorescence - Anti-MCM7/PRL antibody (AB96849)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM7/PRL antibody (AB96849)

Immunoprecipitation - Anti-MCM7/PRL antibody (AB96849)

ChIP - Anti-MCM7/PRL antibody (AB96849)

Western blot - Anti-MCM7/PRL antibody (AB96849)

Western blot - Anti-MCM7/PRL antibody (AB96849)

Western blot - Anti-MCM7/PRL antibody (AB96849)

Western blot - Anti-MCM7/PRL antibody (AB96849)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise