AB179904

Anti-MCM7/PRL antibody - BSA and Azide free

Name: Anti-MCM7/PRL antibody - BSA and Azide free (ab179904) | Abcam
Brand: Abcam Limited
SKU: AB179904
Price: 905 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(4 Publications)

Rabbit Polyclonal MCM7/PRL antibody. Carrier free. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MCM7 aa 550 to C-terminus.

View Alternative Names

CDC47, MCM2, MCM7, DNA replication licensing factor MCM7, CDC47 homolog, P1.1-MCM3

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-MCM7/PRL antibody - BSA and Azide free (AB179904)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MCM7/PRL antibody - BSA and Azide free (AB179904)

Immunofluorecent staining and confocal microscopic analysis of formaldehyde-fixed G₁ phase HeLa cell nucleus, labeling MCM7/PRL using ab179904 at 1/200 dilution, after treatment with protein cross-linking reagent, DSP and chromatin extraction.

Supplier Data

Western blot - Anti-MCM7/PRL antibody - BSA and Azide free (AB179904)

All lanes:

Western blot - Anti-MCM7/PRL antibody - BSA and Azide free (ab179904) at 1/1000 dilution

Lane 1:

SiHa whole cell extract at 100000 Cells

Lane 2:

C33A whole cell extract at 100000 Cells

Lane 3:

WI38 whole cell extract at 100000 Cells

Predicted band size: 81 kDa

false

Supplier Data

Western blot - Anti-MCM7/PRL antibody - BSA and Azide free (AB179904)

All lanes:

Western blot - Anti-MCM7/PRL antibody - BSA and Azide free (ab179904) at 1/2000 dilution

Lane 1:

HeLa whole cell extract

Lane 2:

HeLa whole cell extract treated with 100 nM adriamycin for 24 hours

Lane 3:

NIH 3T3 whole cell extract

Predicted band size: 81 kDa

false

Supplier Data

Immunoprecipitation - Anti-MCM7/PRL antibody - BSA and Azide free (AB179904)

Immunoprecipitation analysis on crude extracts of fibroblast cell line WI38 using ab179904.

Lane 1; Immunoprecipitation with pre-immune serum.

Lane 2; Immnoprecipitation with ab179904 antiserum.

Cells were labeled with S³⁵ methionine and MCM7/PRL was immunoprecipitated with ab179904 followed by SDS-PAGE and autoradiography.

All lanes:

Immunoprecipitation - Anti-MCM7/PRL antibody - BSA and Azide free (ab179904)

Predicted band size: 81 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

ICC/IF, IP, WB

applications

Immunogen

Recombinant Fragment Protein within Human MCM7 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P33993

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

ab179904 is filter-sterilised.

Storage buffer

pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MCM7 (Minichromosome Maintenance Complex Component 7) also known as PRL serves an important mechanical role in DNA replication. It is part of the MCM2-7 complex a helicase essential for the initiation and elongation phases of DNA replication. MCM7 has a molecular mass of approximately 81 kDa and is found expressed in the nucleus of proliferating cells. It is essential for unwinding the DNA helix preparing it for replication.

Biological function summary

MCM7 is involved in maintaining genomic stability and ensuring accurate DNA replication. As part of the larger MCM2-7 hexameric complex it collaborates with other members to form the core component of the pre-replicative complex (pre-RC). By loading onto replication origins MCM7 helps license the DNA for duplication. This function contributes to cell cycle progression particularly the S phase where DNA synthesis occurs.

Pathways

MCM7 plays an important role in DNA replication-related signaling pathways. It functions within the cell cycle and DNA replication pathways interacting closely with other essential proteins like CDC45 and GINS complex. These partnerships facilitate the transition from the G1 to the S phase triggering the replication machinery's activation and ensuring cell division processes continue smoothly.

MCM7 has been associated with various cancers including breast and prostate cancer. Aberrant expression or dysregulation of MCM7 contributes to uncontrolled cell proliferation linking it to tumorigenesis. Also MCM7 interacts with proteins such as cyclin D1 in certain oncogenic pathways emphasizing its role in the progression of cancerous conditions. Understanding these interactions offers insights into potential therapeutic targets for cancer treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed : 25661590, PubMed : 32453425, PubMed : 34694004, PubMed : 34700328, PubMed : 35585232, PubMed : 9305914). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed : 32453425). Required for S-phase checkpoint activation upon UV-induced damage.

See full target information MCM7

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Signal transduction and targeted therapy 7:102 PubMed35414135

2022

LSD1 is required for euchromatic origin firing and replication timing.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Wang,Yunchao Huang,Edith Cheng,Xinhua Liu,Yu Zhang,Jianguo Yang,Jordan T F Young,Grant W Brown,Xiaohan Yang,Yongfeng Shang

The Journal of biological chemistry 277:10354-61 PubMed11779870

2002

Nuclear organization of DNA replication initiation proteins in mammalian cells.

Applications

Unspecified application

Species

Unspecified reactive species

Masatoshi Fujita,Yukio Ishimi,Hiromu Nakamura,Tohru Kiyono,Tatsuya Tsurumi

The Journal of biological chemistry 272:10928-35 PubMed9099751

1997

In vivo interaction of human MCM heterohexameric complexes with chromatin. Possible involvement of ATP.

Applications

Unspecified application

Species

Unspecified reactive species

M Fujita,T Kiyono,Y Hayashi,M Ishibashi

The Journal of biological chemistry 271:4349-54 PubMed8626784

1996

hCDC47, a human member of the MCM family. Dissociation of the nucleus-bound form during S phase.

Applications

Unspecified application

Species

Unspecified reactive species

M Fujita,T Kiyono,Y Hayashi,M Ishibashi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MCM7/PRL antibody - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-MCM7/PRL antibody - BSA and Azide free (AB179904)

Western blot - Anti-MCM7/PRL antibody - BSA and Azide free (AB179904)

Western blot - Anti-MCM7/PRL antibody - BSA and Azide free (AB179904)

Immunoprecipitation - Anti-MCM7/PRL antibody - BSA and Azide free (AB179904)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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