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Rabbit Polyclonal MCM8 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Synthetic Peptide within Human DNA helicase MCM8.

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Images

Western blot - Anti-MCM8 antibody (AB191914), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human DNA helicase MCM8. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UJA3

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR) (PubMed:23401855). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity (PubMed:26215093). Probably by regulating the localization of the MNR complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs (PubMed:23401855). The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression (PubMed:23401855). However, may play a non-essential for DNA replication: may be involved in the activation of the prereplicative complex (pre-RC) during G(1) phase by recruiting CDC6 to the origin recognition complex (ORC) (PubMed:15684404). Probably by regulating HR, plays a key role during gametogenesis (By similarity). Stabilizes MCM9 protein (PubMed:23401855, PubMed:26215093).

Alternative names

Recommended products

Rabbit Polyclonal MCM8 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Synthetic Peptide within Human DNA helicase MCM8.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human DNA helicase MCM8. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UJA3
Purification technique

Affinity purification Immunogen

Concentration
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Purification notes

ab191914 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The Minichromosome Maintenance protein 8 (MCM8) functions as an essential helicase in DNA replication and repair. It forms a hexameric complex that unwinds DNA enabling replication to proceed efficiently. Alternate names include MCM8 homolog (MCM8) and RECQL4 interacting partner. This protein weighs roughly 93 kDa and shows expression mainly in the nucleus especially in proliferating cells.

Biological function summary

Proteins in the MCM family including MCM8 participate in the DNA replication licensing complex ensuring proper DNA replication initiation. They act in concert as part of the replication fork during DNA synthesis stabilizing the junction between unwound single-stranded DNA and replication machinery. MCM8 forms a complex particularly with MCM9 another helicase to facilitate homologous recombination repair a critical DNA repair process ensuring genomic stability.

Pathways

MCM8 engages actively in mechanisms like the DNA damage response and homologous recombination repair pathways. It coordinates with other significant proteins such as BRCA1 to facilitate repairs and prevent genomic instability. MCM8-MCM9 interaction highlights their collective role in homologous recombination connecting it to pathways related to meiotic and mitotic recombination.

Associated diseases and disorders

MCM8 links notably to cancer and primary ovarian insufficiency. Mutations or aberrations in MCM8 disrupt efficient DNA repair mechanisms increasing susceptibility to genomic instability seen in certain cancers. Its interaction with BRCA1 reflects this relation. Defects in MCM8 have implications in fertility-related conditions like primary ovarian insufficiency emphasizing its role in normal reproductive functioning.

Product promise

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1 product image

  • Western blot - Anti-MCM8 antibody (ab191914), expandable thumbnail

    Western blot - Anti-MCM8 antibody (ab191914)

    All lanes: Western blot - Anti-MCM8 antibody (ab191914)

    Lane 1: HEK293T whole cell lysate

    Lane 2: RAW 264.7 whole cell lysate

    Lane 3: H9C2 whole cell lysate

    Predicted band size: 94 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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