Rabbit Polyclonal MCM8 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Synthetic Peptide within Human DNA helicase MCM8.
IgG
Rabbit
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS
Liquid
Polyclonal
WB | |
---|---|
Human | Tested |
Mouse | Tested |
Rat | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR) (PubMed:23401855). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity (PubMed:26215093). Probably by regulating the localization of the MNR complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs (PubMed:23401855). The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression (PubMed:23401855). However, may play a non-essential for DNA replication: may be involved in the activation of the prereplicative complex (pre-RC) during G(1) phase by recruiting CDC6 to the origin recognition complex (ORC) (PubMed:15684404). Probably by regulating HR, plays a key role during gametogenesis (By similarity). Stabilizes MCM9 protein (PubMed:23401855, PubMed:26215093).
C20orf154, MCM8, C20orf154, DNA helicase MCM8, Minichromosome maintenance 8
Rabbit Polyclonal MCM8 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Synthetic Peptide within Human DNA helicase MCM8.
IgG
Rabbit
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS
Liquid
Polyclonal
Affinity purification Immunogen
ab191914 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
The Minichromosome Maintenance protein 8 (MCM8) functions as an essential helicase in DNA replication and repair. It forms a hexameric complex that unwinds DNA enabling replication to proceed efficiently. Alternate names include MCM8 homolog (MCM8) and RECQL4 interacting partner. This protein weighs roughly 93 kDa and shows expression mainly in the nucleus especially in proliferating cells.
Proteins in the MCM family including MCM8 participate in the DNA replication licensing complex ensuring proper DNA replication initiation. They act in concert as part of the replication fork during DNA synthesis stabilizing the junction between unwound single-stranded DNA and replication machinery. MCM8 forms a complex particularly with MCM9 another helicase to facilitate homologous recombination repair a critical DNA repair process ensuring genomic stability.
MCM8 engages actively in mechanisms like the DNA damage response and homologous recombination repair pathways. It coordinates with other significant proteins such as BRCA1 to facilitate repairs and prevent genomic instability. MCM8-MCM9 interaction highlights their collective role in homologous recombination connecting it to pathways related to meiotic and mitotic recombination.
MCM8 links notably to cancer and primary ovarian insufficiency. Mutations or aberrations in MCM8 disrupt efficient DNA repair mechanisms increasing susceptibility to genomic instability seen in certain cancers. Its interaction with BRCA1 reflects this relation. Defects in MCM8 have implications in fertility-related conditions like primary ovarian insufficiency emphasizing its role in normal reproductive functioning.
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All lanes: Western blot - Anti-MCM8 antibody (ab191914)
Lane 1: HEK293T whole cell lysate
Lane 2: RAW 264.7 whole cell lysate
Lane 3: H9C2 whole cell lysate
Predicted band size: 94 kDa
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