AB235335

Anti-MCM9 antibody

Name: Anti-MCM9 antibody (ab235335) | Abcam
Brand: Abcam Limited
SKU: AB235335
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal MCM9 antibody. Suitable for WB and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MCM9 aa 1-400.

View Alternative Names

C6orf61, MCMDC1, MCM9, DNA helicase MCM9, hMCM9, Mini-chromosome maintenance deficient domain-containing protein 1, Minichromosome maintenance 9

1 Images

Supplier Data

Western blot - Anti-MCM9 antibody (AB235335)

All lanes:

Western blot - Anti-MCM9 antibody (ab235335) at 1/1000 dilution

Lane 1:

293 whole cell lysate

Lane 2:

MCF7 whole cell lysate

Lane 3:

K562 whole cell lysate

Lane 4:

A549 whole cell lysate

Lane 5:

Mouse Brain tissue lysate

Secondary

All lanes:

Western blot at 1/50000 dilution

Observed band size: 127 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human MCM9 aa 1-400. The exact immunogen used to generate this antibody is proprietary information.

Q9NXL9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCM9 also known as Minichromosome Maintenance Complex Component 9 plays a mechanical role in DNA replication. It is a component of the MCM2-7 complex which is important for the formation of the helicase that unwinds DNA during replication. MCM9 has a molecular mass of approximately 97 kDa. The expression of MCM9 is mostly found in fast-dividing cells like cancer cells as well as in tissues such as ovary and testis where cell proliferation is high.

Biological function summary

MCM9 is involved in homologous recombination repair of DNA double-strand breaks. It participates in the pre-replication complex formation and in the activation of the replication checkpoint. As part of the MCM2-7 helicase complex MCM9 maintains genomic stability by ensuring accurate DNA replication and repair. This highlights its critical role in the maintenance of DNA integrity across cell divisions particularly in proliferating cells.

Pathways

MCM9 integrates into the DNA damage response and repair mechanisms. It works alongside proteins like RAD51 a recombinase in the homologous recombination pathway for repairing double-strand breaks. MCM9 also interacts with the ATR signaling pathway which becomes activated in response to DNA damage and works to halt the cell cycle to allow repair.

Aberrations in MCM9 activity contribute to conditions such as cancer and hereditary breast and ovarian cancer syndrome. Its malfunction can lead to genomic instability and unchecked cell proliferation due to impaired DNA repair mechanisms. Connections have been drawn between MCM9 and BRCA1 another DNA repair protein highlighting its involvement in maintaining DNA stability and its association with cancer predispositions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR) (PubMed : 23401855). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity (PubMed : 26215093). Probably by regulating the localization of the MRN complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs (PubMed : 23401855). Acts as a helicase in DNA mismatch repair (MMR) following DNA replication errors to unwind the mismatch containing DNA strand (PubMed : 26300262). In addition, recruits MLH1, a component of the MMR complex, to chromatin (PubMed : 26300262). The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression (PubMed : 23401855). Probably by regulating HR, plays a key role during gametogenesis (By similarity).

See full target information MCM9

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 11:292 PubMed40593474

2025

MCM9 deficiency impairs DNA damage repair during spermatogenesis, leading to Sertoli cell-only syndrome in humans.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Sha,Xin Zhang,Hao Geng,Yuqian Li,Xun Xia,Guotong Li,Rong Hua,Kuokuo Li,Yang Gao,Qunshan Shen,Rui Guo,Yuping Xu,Xiaojin He,Yunxia Cao,Mingxi Liu,Huan Wu

Journal of cellular biochemistry 120:9506-9513 PubMed30520128

2018

Quantitative proteomics using SILAC-MS identifies N-acetylcysteine-solution-triggered reversal response of renal cell carcinoma cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Cuihong Zhao,Jianhua Chen,Yong Ling,Shenghai Wang

View all publications

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Product promise

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Anti-MCM9 antibody

Western blot - Anti-MCM9 antibody (AB235335)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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