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AB9858

Anti-MCP1 antibody [2.2-4H5-1A11]

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(1 Review)

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(4 Publications)

Mouse Monoclonal MCP1 antibody. Suitable for ELISA, WB, sELISA, IHC-P and reacts with Recombinant full length protein - Human, Recombinant fragment - Human, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CCL2.

View Alternative Names

MCP1, SCYA2, CCL2, C-C motif chemokine 2, HC11, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, Small-inducible cytokine A2, MCAF, MCP-1

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Sandwich ELISA - Anti-MCP1 antibody [2.2-4H5-1A11] (AB9858)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-MCP1 antibody [2.2-4H5-1A11] (AB9858)

In a sandwich ELISA (assuming 100 μl/well), a concentration of 1.0-2.0 μg/ml of this antibody will detect at least 100 pg/mL of Recombinant Human MCP-1 when used with Biotin Anti-MCP1 antibody (ab271250) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.

Sandwich ELISA - Anti-MCP1 antibody [2.2-4H5-1A11] (AB9858)
  • sELISA

Unknown

Sandwich ELISA - Anti-MCP1 antibody [2.2-4H5-1A11] (AB9858)

Standard curve for MCP1 (Analyte : ab73866); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal to MCP1 (ab9858) at 1μg/ml and Detector Antibody Rabbit polyclonal to MCP1 (ab9669) at 0.5μg/ml.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2.2-4H5-1A11

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

ELISA, IHC-P, WB, sELISA

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CCL2.

P13500

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Reactivity data

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You may be interested in:

AB179886

Human MCP-1 ELISA Kit

0

0 Reviews

View product

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

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Properties and storage information

Form
Lyophilized
Reconstitution
Reconstitute with 500µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Purification technique
Affinity purification Protein A
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCP-1 also known as CCL2 is a chemokine involved in the recruitment of monocytes to sites of injury and inflammation. This protein consists of 76 amino acids and has a molecular weight of about 11 kDa. MCP-1 is expressed in a wide range of cell types including endothelial cells fibroblasts and monocytes. It acts by binding to the chemokine receptor CCR2 stimulating intracellular signaling pathways that mediate cell migration.
Biological function summary

The role of MCP-1 is significant in guiding immune cells to areas requiring immune response. It does not form part of a complex but operates as a singular entity to affect immune signaling. MCP-1 influences the behavior of monocytes and macrophages by inducing chemotaxis and activating their effector functions. Through this mechanism it aids in the inflammatory response and tissue repair.

Pathways

MCP-1 participates in the inflammatory pathways by cooperating with key proteins like CCR2. It facilitates the JAK-STAT signaling pathway which is vital for transmitting extracellular information into cellular responses. The interaction of MCP-1 with CCR2 influences the activation of downstream proteins like STAT3 enabling various immune responses. MCP-1 thereby plays a central role in modulating inflammation-related pathways.

MCP-1 has a connection to inflammatory bowel disease and atherosclerosis. In such conditions elevated levels of MCP-1 can exacerbate inflammatory responses by recruiting monocytes to dysfunctional sites worsening tissue damage. Through its interaction with CCR2 MCP-1 becomes a pivotal factor in chronic inflammation. Targeting this MCP-1/CCR2 axis can provide therapeutic opportunities for modulating disease progression in these inflammatory diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a ligand for C-C chemokine receptor CCR2 (PubMed : 10529171, PubMed : 10587439, PubMed : 9837883). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed : 10587439, PubMed : 9837883). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (PubMed : 8195247, PubMed : 8627182, PubMed : 9792674). May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis (PubMed : 8107690).
See full target information CCL2
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Diagnostics (Basel, Switzerland) 11: PubMed34067481

2021

Elevation of CD40/CD40L Inflammatory Pathway Molecules in Carotid Plaques from Moderate-and-Severe Obstructive Sleep Apnea Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Ewa Migacz,Wioletta Olejarz,Alicja Głuszko,Katarzyna Bednarek-Rajewska,Robert Proczka,David F Smith,Stacey L Ishman,Wojciech Kukwa

Oncology letters 15:7760-7768 PubMed29725470

2018

Mutant monocyte chemoattractant protein-1 protein (7ND) inhibits osteoclast differentiation and reduces oral squamous carcinoma cell bone invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Shuyu Luo,Chuanxiang Zhou,Jianming Zhang,Mengshan Chen,Hongjie Li,Shanchuan Zheng,Jingjing Quan

PloS one 9:e88952 PubMed24586454

2014

Monocyte chemotactic protein 1 (MCP-1) modulates pro-survival signaling to promote progression of head and neck squamous cell carcinoma.

Applications

IHC-P

Species

Human

Wen-Tsai Ji,Hau-Ren Chen,Chun-Hsuan Lin,Jeng-Woei Lee,Ching-Chih Lee

Frontiers in aging neuroscience 5:19 PubMed23675346

2013

Thrombin, a mediator of cerebrovascular inflammation in AD and hypoxia.

Applications

IHC-P

Species

Mouse

Debjani Tripathy,Alma Sanchez,Xiangling Yin,Jinhua Luo,Joseph Martinez,Paula Grammas
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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