JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB21396

Anti-MCP1 antibody [2H5]

5

(1 Review)

|

(14 Publications)

Armenian hamster Monoclonal MCP1 antibody. Suitable for ELISA and reacts with Recombinant fragment - Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Ccl2.

View Alternative Names

Je, Mcp1, Scya2, Ccl2, C-C motif chemokine 2, Monocyte chemoattractant protein 1, Monocyte chemotactic protein 1, Platelet-derived growth factor-inducible protein JE, Small-inducible cytokine A2, MCP-1

1 Images
ELISA - Anti-MCP1 antibody [2H5] (AB21396)
  • ELISA

Unknown

ELISA - Anti-MCP1 antibody [2H5] (AB21396)

ab21396 staining human MCP-1 by ELISA. Image shows a standard curve obtained with increasing concentrations of human MCP-1.

Key facts

Host species

Armenian hamster

Clonality

Monoclonal

Clone number

2H5

Isotype

IgG

Carrier free

No

Applications

ELISA

applications

Immunogen

Recombinant Full Length Protein corresponding to Mouse Ccl2.

P10148

Specificity

This antibody is specific to mouse MCP-1.

style
left-column

Alternative Products

Primary Antibodies

AB214819

Anti-MCP1 antibody [EPR21025]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunocytochemistry - Anti-MCP1 antibody [EPR21025] (AB214819)

  • 0
  • 0
Primary Antibodies

AB242013

Anti-MCP1 antibody [EPR21025] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunocytochemistry - Anti-MCP1 antibody [EPR21025] - BSA and Azide free (AB242013)

  • 0
  • 0
Primary Antibodies

AB246795

Anti-MCP1 antibody [EPR21025] - Low endotoxin, Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Flow Cytometry - Anti-MCP1 antibody [EPR21025] - Low endotoxin, Azide free (AB246795)

  • 0
  • 0

Complementary Products

Assay Kits

AB179886

Human MCP-1 ELISA Kit

Recombinant|SimpleStep

ELISA - Human MCP-1 ELISA Kit (AB179886)

  • 0
  • 0
Primary Antibodies

AB183218

Anti-TNF alpha antibody [EPR19147]

20ul selling size|KO Validated|RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-TNF alpha antibody [EPR19147] (AB183218)

  • 5
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment - Human": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "5-10 µg/mL", "ELISA-species-notes": "<p>To obtain a linear standard curve, serial dilutions of MCP-1 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate.</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purity >95% by SDS-PAGE.
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCP-1 also known as CCL2 is a chemokine involved in the recruitment of monocytes to sites of injury and inflammation. This protein consists of 76 amino acids and has a molecular weight of about 11 kDa. MCP-1 is expressed in a wide range of cell types including endothelial cells fibroblasts and monocytes. It acts by binding to the chemokine receptor CCR2 stimulating intracellular signaling pathways that mediate cell migration.
Biological function summary

The role of MCP-1 is significant in guiding immune cells to areas requiring immune response. It does not form part of a complex but operates as a singular entity to affect immune signaling. MCP-1 influences the behavior of monocytes and macrophages by inducing chemotaxis and activating their effector functions. Through this mechanism it aids in the inflammatory response and tissue repair.

Pathways

MCP-1 participates in the inflammatory pathways by cooperating with key proteins like CCR2. It facilitates the JAK-STAT signaling pathway which is vital for transmitting extracellular information into cellular responses. The interaction of MCP-1 with CCR2 influences the activation of downstream proteins like STAT3 enabling various immune responses. MCP-1 thereby plays a central role in modulating inflammation-related pathways.

MCP-1 has a connection to inflammatory bowel disease and atherosclerosis. In such conditions elevated levels of MCP-1 can exacerbate inflammatory responses by recruiting monocytes to dysfunctional sites worsening tissue damage. Through its interaction with CCR2 MCP-1 becomes a pivotal factor in chronic inflammation. Targeting this MCP-1/CCR2 axis can provide therapeutic opportunities for modulating disease progression in these inflammatory diseases.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Acts as a ligand for C-C chemokine receptor CCR2 (By similarity). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (By similarity). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (By similarity). Plays an important role in mediating peripheral nerve injury-induced neuropathic pain (PubMed : 29993042). Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (PubMed : 29993042).
See full target information Ccl2
style
left-column

Publications (14)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 51: PubMed37026512

2023

STAT3/NF‑κB decoy oligodeoxynucleotides inhibit atherosclerosis through regulation of the STAT/NF‑κB signaling pathway in a mouse model of atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hyun-Jin An,Mi-Gyeong Gwon,Hyemin Gu,Seongjae Bae,Jaechan Leem,Jin Bae Lee,Kwan-Kyu Park

Renal failure 43:465-476 PubMed33678127

2021

Calcium-sensing receptor promotes calcium oxalate crystal adhesion and renal injury in Wistar rats by promoting ROS production and subsequent regulation of PS ectropion, OPN, KIM-1, and ERK expression.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoran Li,Siyu Chen,Demei Feng,Yuqiang Fu,Huang Wu,Jianzhong Lu,Junsheng Bao

Technology and health care : official journal of the European Society for Engineering and Medicine 29:91-101 PubMed33682749

2021

The roles of metastasis-related proteins in the development of giant cell tumor of bone, osteosarcoma and Ewing's sarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Dou,Tianrui Chen,Qiubo Chu,Guirong Zhang,Zhaoli Meng

European journal of pharmacology 880:173175 PubMed32416183

2020

Partial MHC/neuroantigen peptide constructs attenuate methamphetamine-seeking and brain chemokine (C-C motif) ligand 2 levels in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer M Loftis,Tommy Navis,Jonathan Taylor,Rebekah Hudson,Ulziibat Person,K Matthew Lattal,Arthur A Vandenbark,Renee Shirley,Marilyn Huckans

Cell reports 30:1923-1934.e4 PubMed32049021

2020

CCL2 and CXCL12 Derived from Mesenchymal Stromal Cells Cooperatively Polarize IL-10+ Tissue Macrophages to Mitigate Gut Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jayeeta Giri,Rahul Das,Emily Nylen,Raghavan Chinnadurai,Jacques Galipeau

Bioscience reports 39: PubMed30808715

2019

gene suppresses intracranial aneurysm formation and rupture through inhibiting the NF-κB signaling pathway mediated inflammatory response.

Applications

Unspecified application

Species

Unspecified reactive species

Xian-Liang Lai,Zhi-Feng Deng,Xin-Gen Zhu,Zhi-Hua Chen

International journal of molecular medicine 43:1611-1622 PubMed30720053

2019

Metformin prevents nephrolithiasis formation by inhibiting the expression of OPN and MCP-1 in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Xiong Yang,Tong Yang,Jie Li,Rui Yang,Shiyong Qi,Yang Zhao,Liang Li,Jingjin Li,Xuening Zhang,Kuo Yang,Yong Xu,Chunyu Liu

Oxidative medicine and cellular longevity 2016:5203801 PubMed27965733

2016

Calcium Oxalate Induces Renal Injury through Calcium-Sensing Receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoran Li,Junhai Ma,Wei Shi,Yu Su,Xu Fu,Yanlin Yang,Jianzhong Lu,Zhongjin Yue

Scientific reports 6:33705 PubMed27659793

2016

Downregulation of angiotensin type 1 receptor and nuclear factor-κB by sirtuin 1 contributes to renoprotection in unilateral ureteral obstruction.

Applications

Unspecified application

Species

Unspecified reactive species

Shao-Yu Yang,Shuei-Liong Lin,Yung-Ming Chen,Vin-Cent Wu,Wei-Shiung Yang,Kwan-Dun Wu

Journal of neuropathology and experimental neurology 71:468-79 PubMed22588385

2012

Diphenylarsinic acid increased the synthesis and release of neuroactive and vasoactive peptides in rat cerebellar astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Takayuki Negishi,Masaki Takahashi,Yuki Matsunaga,Seishiro Hirano,Tomoko Tashiro
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com