Anti-MCP1 antibody (ab25124) is a rabbit polyclonal antibody that is used to detect MCP1 in Western Blot, Flow Cytometry, IHC-P, Neut. Suitable for Mouse, Rat samples.
- Over 160 publications
- Trusted since 2005
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- Journal of oral microbiology 16:22923752023MAT2A inhibition suppresses inflammation in -infected human gingival fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLishan Jiang,Jingwen Li,Kun Ji,Lang Lei,Houxuan LiPubMed 38130504
- Nature 623:792-8022023CD201 fascia progenitors choreograph injury repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDonovan Correa-Gallegos,Haifeng Ye,Bikram Dasgupta,Aydan Sardogan,Safwen Kadri,Ravinder Kandi,Ruoxuan Dai,Yue Lin,Robert Kopplin,Disha Shantaram Shenai,Juliane Wannemacher,Ryo Ichijo,Dongsheng Jiang,Maximilian Strunz,Meshal Ansari,Illias Angelidis,Herbert B Schiller,Thomas Volz,Hans-Günther Machens,Yuval RinkevichPubMed 37968392
- Cells 12:2023Probiotic and Muscadine Grape Extract Interventions Shift the Gut Microbiome and Improve Metabolic Parameters in Female C57BL/6 Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTiffany M Newman,Adam S Wilson,Kenysha Y J Clear,E Ann Tallant,Patricia E Gallagher,Katherine L CookPubMed 37998334
- Arthritis research & therapy 25:1682023Effects of low-intensity exercise on spontaneously developed knee osteoarthritis in male senescence-accelerated mouse prone 8.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKosuke Norimatsu,Kazuki Nakanishi,Toshiro Ijuin,Shotaro Otsuka,Seiya Takada,Akira Tani,Ryoma Matsuzaki,Teruki Matsuoka,Harutoshi SakakimaPubMed 37710278
- Cell discovery 9:942023CCL2-mediated inflammatory pathogenesis underlies high myopia-related anxiety.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiangjia Zhu,Jiaqi Meng,Chaofeng Han,Qingfeng Wu,Yu Du,Jiao Qi,Ling Wei,Hao Li,Wenwen He,Keke Zhang,Yi LuPubMed 37699875
- Phytotherapy research : PTR 37:4133-41482023Astragaloside IV attenuates high glucose-induced NF-κB-mediated inflammation through activation of PI3K/AKT-ERK-dependent Nrf2/ARE signaling pathway in glomerular mesangial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXue Su,Hengjiang Guo,Yuying Zhou,Aili Cao,Qian Shen,Bingbing Zhu,Xingmei Yao,Yunman Wang,Hao Wang,Li WangPubMed 37189016
- Diabetes & metabolism journal 47:653-6672023CycloZ Improves Hyperglycemia and Lipid Metabolism by Modulating Lysine Acetylation in KK-Ay Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJongsu Jeon,Dohyun Lee,Bobae Kim,Bo-Yoon Park,Chang Joo Oh,Min-Ji Kim,Jae-Han Jeon,In-Kyu Lee,Onyu Park,Seoyeong Baek,Chae Won Lim,Dongryeol Ryu,Sungsoon Fang,Johan Auwerx,Kyong-Tai Kim,Hoe-Yune JungPubMed 37098411
- Proceedings of the National Academy of Sciences of the United States of America 120:e22198851202023Consequences of poly(ethylene oxide) and poloxamer P188 on transcription in healthy and stressed myoblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdelyn A Crabtree,Natalie Boehnke,Frank S Bates,Benjamin J HackelPubMed 37094151
- Antioxidants (Basel, Switzerland) 12:2023Epigallocatechin-3-Gallate Attenuates Leukocyte Infiltration in 67-kDa Laminin Receptor-Dependent and -Independent Pathways in the Rat Frontoparietal Cortex following Status Epilepticus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJi-Eun Kim,Duk-Shin Lee,Tae-Cheon KangPubMed 37107345
- Frontiers in cardiovascular medicine 10:11521242023Injury to the tunica media initiates atherogenesis in the presence of hyperlipidemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHanane Belhoul-Fakir,Jiansha Wu,Yen L Yeow,Gabrielle C Musk,Helen Kershaw,Evan Ingley,Bichen Sophie Zhao,Christopher M Reid,Christopher Lagat,Brian Evans,Peter L Thompson,Michael L Brown,Juliana Hamzah,Shirley JansenPubMed 37063951
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99.95% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Rat Ccl2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P14844
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | Neut | |
|---|---|---|---|---|
| Mouse | Expected | Tested | Expected | Expected |
| Rat | Expected | Predicted | Expected | Expected |
Flow Cyt
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Used at a dilution of 1/100 for 30 min on rat primary mixed cortical cells (see Abreview for further information). ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info - | Notes Used at a dilution of 1/100 for 30 min on rat primary mixed cortical cells (see Abreview for further information). ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Neut
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Function
Acts as a ligand for C-C chemokine receptor CCR2 (By similarity). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (By similarity). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (By similarity). Plays an important role in mediating peripheral nerve injury-induced neuropathic pain (By similarity). Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (By similarity).
Alternative names
Je, Mcp1, Scya2, Ccl2, C-C motif chemokine 2, Immediate-early serum-responsive protein JE, Monocyte chemoattractant protein 1, Monocyte chemotactic protein 1, Small-inducible cytokine A2, MCP-1
Recommended products
Anti-MCP1 antibody (ab25124) is a rabbit polyclonal antibody that is used to detect MCP1 in Western Blot, Flow Cytometry, IHC-P, Neut. Suitable for Mouse, Rat samples.
- Over 160 publications
- Trusted since 2005
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99.95% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Rat Ccl2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P14844
- Purification technique
- Affinity purification Protein A
- Specificity
ab25124 reacts with both rat MCP1 and its mouse homolog JE.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-MCP1 antibody (ab25124) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Neutralising in Mouse, Rat samples.
Trusted by the scientific community
Anti-MCP1 (ab25124) was first used in a scientific publication in 2005 and has been cited over 160 times in peer-reviewed journals.
Reviewed by scientists
Anti-MCP1 (ab25124) has over 5 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MCP-1 also known as CCL2 is a chemokine involved in the recruitment of monocytes to sites of injury and inflammation. This protein consists of 76 amino acids and has a molecular weight of about 11 kDa. MCP-1 is expressed in a wide range of cell types including endothelial cells fibroblasts and monocytes. It acts by binding to the chemokine receptor CCR2 stimulating intracellular signaling pathways that mediate cell migration.
- Biological function summary
The role of MCP-1 is significant in guiding immune cells to areas requiring immune response. It does not form part of a complex but operates as a singular entity to affect immune signaling. MCP-1 influences the behavior of monocytes and macrophages by inducing chemotaxis and activating their effector functions. Through this mechanism it aids in the inflammatory response and tissue repair.
- Pathways
MCP-1 participates in the inflammatory pathways by cooperating with key proteins like CCR2. It facilitates the JAK-STAT signaling pathway which is vital for transmitting extracellular information into cellular responses. The interaction of MCP-1 with CCR2 influences the activation of downstream proteins like STAT3 enabling various immune responses. MCP-1 thereby plays a central role in modulating inflammation-related pathways.
- Associated diseases and disorders
MCP-1 has a connection to inflammatory bowel disease and atherosclerosis. In such conditions elevated levels of MCP-1 can exacerbate inflammatory responses by recruiting monocytes to dysfunctional sites worsening tissue damage. Through its interaction with CCR2 MCP-1 becomes a pivotal factor in chronic inflammation. Targeting this MCP-1/CCR2 axis can provide therapeutic opportunities for modulating disease progression in these inflammatory diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Western blot - Anti-MCP1 antibody (ab25124)
MCP1 Western blot staining using rabbit Anti-MCP1 antibody
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 28955950).
Panel A is applied with Anti-MCP1 antibody [RM1100] (Anti-MCP1 antibody [RM1100] ab315478) at 1/1000 dilution and panel B is applied with Anti-MCP1 antibody (ab25124) at 1/1000 dilution.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: 26 seconds (panel A);180 seconds (panel B).
All lanes: Western blot - Anti-MCP1 antibody [RM1100] (Anti-MCP1 antibody [RM1100] ab315478) at 1/1000 dilution
Lane 1: Untreated RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 2: RAW 264.7 treated with 100ng/ml Lipopolysaccharides (LPS) for 7 hours, then 1µg/ml Brefeldin A was added for additional 3 hours whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 20 kDa, 36 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCP1 antibody (ab25124)
Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling MCP1 with Anti-MCP1 antibody [RM1100] ab315478 at 1/3000 (0.172 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: Anti-MCP1 antibody [RM1100] ab315478 showed no staining on mouse skeletal muscle (ab), while ab25124 showed non-specific staining on the mouse skeletal muscle.
The image on the left is applied with Anti-MCP1 antibody [RM1100] (Anti-MCP1 antibody [RM1100] ab315478) at 1/3000 dilution and the image on the right is applied with Anti-MCP1 antibody (ab25124) at 1/1500 dilution.
The section was incubated with Anti-MCP1 antibody [RM1100] ab315478 or ab25124 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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