Anti-MCP1 antibody

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MCP1 antibody (AB7202)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized parental HAP1(Green) / MIF KO HAP1(MIF knockout human chronic myelogenous leukemia near-haploid cell line, Magenta) cells labelling MIF with ab322733 at 1/500 dilution (0.1ug) / Magenta and Green compared with a Rabbit monoclonal IgG (ab172730) (Black) and Grey isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

For ab322733, positive staining occurred in HAP1 cells and no staining in MIF KO HAP1. The control batch (ab7202) showed similar staining pattern. In addition, ab322733 showed stronger staining than ab7207 in HAP1 cells.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCP1 antibody (AB7202)

Immunohistochemical analysis of paraffin-embedded LPS-treated rat lung tissue stained for MCP1 using ab7202 at 1/300 dilution.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MCP1 antibody (AB7202)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling MIF with ab322733 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

For ab322733, positive staining occurred in RAW 264.7 cells. The control batch (ab7202) showed similar staining pattern. In addition, ab322733 showed stronger staining than ab7207 in RAW 264.7 cells.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MCP1 antibody (AB7202)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C6 (rat glial tumor glial cell) cells labelling MIF with ab322733 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

For ab322733, positive staining occurred in C6 cells. The control batch (ab7202) showed similar staining pattern. In addition, ab322733 showed stronger staining than ab7207 in C6 cells.

• WB

Supplier Data

Western blot - Anti-MCP1 antibody (AB7202)

Western blot analysis of RAW cell lysates labelling MCP1 with ab7202.

All lanes:

Western blot - Anti-MCP1 antibody (ab7202)

Lane 1:

LPS-stimulated RAW cell lysate

Lane 2:

Rat MCP1-transfected RAW cell lysate

Predicted band size: 11 kDa

false

• WB

CiteAb

Western blot - Anti-MCP1 antibody (AB7202)

MCP1 western blot using anti-MCP1 antibody ab7202. Publication image and figure legend from Gao, W., Tang, X., et al., 2014, Evid Based Complement Alternat Med, PubMed 24396391.

ab7202 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab7202 please see the product overview.

The expression of CCL2 and CXCL8 proteins in rat endometrium on D6, D8, and D10 of pregnancy. (a) Representative blots of CCL2 and CXCL8. (b and c) Statistical analysis of CCL2 (b) and CXCL8 (c) in rat endometrium. The relative intensity was determined by the ratio of interested protein to its corresponding internal control (β-actin) as measured by densitometry. Data are presented as mean ± SD (n = 6). *p < 0.05, **p < 0.01 compared with N; #p < 0.05, ##p < 0.01 compared with M.

false