AB73680

Anti-MCP1 antibody

(1 Review)

(11 Publications)

Rabbit Polyclonal MCP1 antibody. Suitable for WB and reacts with Human samples. Cited in 11 publications.

View Alternative Names

MCP1, SCYA2, CCL2, C-C motif chemokine 2, HC11, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, Small-inducible cytokine A2, MCAF, MCP-1

1 Images

Unknown

Western blot - Anti-MCP1 antibody (AB73680)

The expression profile observed is consistent with what has been described in the literature (e.g. PMID 12957789). We believe the 50kDa band is a glycosylated form of Human MCP1.

All lanes:

Western blot - Anti-MCP1 antibody (ab73680) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 11 kDa

Observed band size: 125 kDa,17 kDa,50 kDa,80 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCP-1 also known as CCL2 is a chemokine involved in the recruitment of monocytes to sites of injury and inflammation. This protein consists of 76 amino acids and has a molecular weight of about 11 kDa. MCP-1 is expressed in a wide range of cell types including endothelial cells fibroblasts and monocytes. It acts by binding to the chemokine receptor CCR2 stimulating intracellular signaling pathways that mediate cell migration.

Biological function summary

The role of MCP-1 is significant in guiding immune cells to areas requiring immune response. It does not form part of a complex but operates as a singular entity to affect immune signaling. MCP-1 influences the behavior of monocytes and macrophages by inducing chemotaxis and activating their effector functions. Through this mechanism it aids in the inflammatory response and tissue repair.

Pathways

MCP-1 participates in the inflammatory pathways by cooperating with key proteins like CCR2. It facilitates the JAK-STAT signaling pathway which is vital for transmitting extracellular information into cellular responses. The interaction of MCP-1 with CCR2 influences the activation of downstream proteins like STAT3 enabling various immune responses. MCP-1 thereby plays a central role in modulating inflammation-related pathways.

MCP-1 has a connection to inflammatory bowel disease and atherosclerosis. In such conditions elevated levels of MCP-1 can exacerbate inflammatory responses by recruiting monocytes to dysfunctional sites worsening tissue damage. Through its interaction with CCR2 MCP-1 becomes a pivotal factor in chronic inflammation. Targeting this MCP-1/CCR2 axis can provide therapeutic opportunities for modulating disease progression in these inflammatory diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a ligand for C-C chemokine receptor CCR2 (PubMed : 10529171, PubMed : 10587439, PubMed : 9837883). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed : 10587439, PubMed : 9837883). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (PubMed : 8195247, PubMed : 8627182, PubMed : 9792674). May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis (PubMed : 8107690).

See full target information CCL2

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Cancer medicine 12:16221-16230 PubMed37341066

2023

MCP-1 expression in breast cancer and its association with distant relapse.

Applications

Unspecified application

Species

Unspecified reactive species

Bridie S Mulholland,Pierre Hofstee,Ewan K A Millar,Dana Bliuc,Sandra O'Toole,Mark R Forwood,Michelle M McDonald

Nature communications 12:6867 PubMed34824203

2021

Interrogation of the microenvironmental landscape in spinal ependymomas reveals dual functions of tumor-associated macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Qianqian Zhang,Sijin Cheng,Yongzhi Wang,Mengdi Wang,Yufeng Lu,Zengqi Wen,Yuxin Ge,Qiang Ma,Youqiao Chen,Yaowu Zhang,Ren Cao,Min Li,Weihao Liu,Bo Wang,Qian Wu,Wenqing Jia,Xiaoqun Wang

Bone research 9:43 PubMed34588427

2021

Inflammatory conversion of quiescent osteoblasts by metastatic breast cancer cells through pERK1/2 aggravates cancer-induced bone destruction.

Applications

Unspecified application

Species

Unspecified reactive species

Jungho Back,Minh Nam Nguyen,Lu Li,Saelim Lee,Inkyu Lee,Fancheng Chen,Lauren Gillinov,Yeon-Ho Chung,Kareme D Alder,Hyuk-Kwon Kwon,Kristin E Yu,Christopher M Dussik,Zichen Hao,Michael J Flores,Yoseph Kim,Izuchukwu K Ibe,Alana M Munger,Sung Wook Seo,Francis Y Lee

Molecular medicine reports 24: PubMed34396447

2021

CXCL16 may be a predisposing factor to atherosclerosis: An animal study.

Applications

Unspecified application

Species

Unspecified reactive species

Junbi Zhao,Menglin Yang,Jie Wu

Experimental and therapeutic medicine 21:564 PubMed33850536

2021

C-C motif chemokine ligand 2/C-C receptor 2 is associated with glioma recurrence and poor survival.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuan Yang,Junpeng Zhang,Xin Zhang,Lifeng Miao,Wei Zhang,Zheng Jiang,Wei Zhou

Theranostics 11:3624-3641 PubMed33664852

2021

Genome-wide CRISPR/Cas9 knockout screening uncovers a novel inflammatory pathway critical for resistance to arginine-deprivation therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Ying Chu,Yi-Ching Lee,Cheng-Han Hsieh,Chi-Tai Yeh,Tsu-Yi Chao,Po-Hung Chen,I-Hsuan Lin,Tsung-Han Hsieh,Jing-Wen Shih,Chia-Hsiung Cheng,Che-Chang Chang,Ping-Sheng Lin,Yuan-Li Huang,Tsung-Ming Chen,Yun Yen,David K Ann,Hsing-Jien Kung

Frontiers in oncology 10:564298 PubMed33330033

2020

Forkhead Box Q1 Is Critical to Angiogenesis and Macrophage Recruitment of Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Tang,Ji Zheng,Xuan Bai,Ke-Lin Yue,Jian-Hua Liang,Dan-Yang Li,Lin-Ping Wang,Jin-Li Wang,Qiang Guo

Journal of molecular neuroscience : MN 70:1728-1741 PubMed32495004

2020

Induction of SPARC on Oxidative Stress, Inflammatory Phenotype Transformation, and Apoptosis of Human Brain Smooth Muscle Cells Via TGF-β1-NOX4 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xianjun Tan,Tao Li,Shaowei Zhu,Weiying Zhong,Feng Li,Yunyan Wang

Hepatology communications 2:230-236 PubMed29507898

2018

Role of monocyte chemoattractant protein-1 in liver fibrosis with transient myeloproliferative disorder in down syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Kenichiro Kobayashi,Takako Yoshioka,Jun Miyauchi,Atsuko Nakazawa,Nobutaka Kiyokawa,Toshiro Maihara,Ikuya Usami

The European respiratory journal 44:951-62 PubMed25034560

2014

Comprehensive analysis of inflammatory markers in chronic thromboembolic pulmonary hypertension patients.

Applications

IHC

Species

Unspecified reactive species

Diana Zabini,Akos Heinemann,Vasile Foris,Chandran Nagaraj,Patrick Nierlich,Zoltán Bálint,Grazyna Kwapiszewska,Irene M Lang,Walter Klepetko,Horst Olschewski,Andrea Olschewski

View all publications

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Product promise

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Anti-MCP1 antibody

Western blot - Anti-MCP1 antibody (AB73680)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise