Rabbit Polyclonal MCP1 antibody. Suitable for WB, sELISA and reacts with Recombinant full length protein - Human samples. Cited in 103 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CCL2.
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- Immunity, inflammation and disease 11:e10062023SAMD1 attenuates antiphospholipid syndrome-induced pregnancy complications.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRan An,Yanqi Yang,Lei Liu,Peiling LiPubMed 37904675
- Experimental & molecular medicine 55:1713-17192023Establishment of a humanized mouse model of keloid diseases following the migration of patient immune cells to the lesion: Patient-derived keloid xenograft (PDKX) model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesA Ram Lee,Seon-Yeong Lee,Jeong Won Choi,In Gyu Um,Hyun Sik Na,Jung Ho Lee,Mi-La ChoPubMed 37524866
- Biomolecules 13:2023Oscillating Glucose Induces the Increase in Inflammatory Stress through Ninjurin-1 Up-Regulation and Stimulation of Transport Proteins in Human Endothelial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura Toma,Gabriela M Sanda,Camelia S Stancu,Loredan S Niculescu,Mina Raileanu,Anca V SimaPubMed 37189375
- Arthritis research & therapy 24:2822022Early removal of the infrapatellar fat pad/synovium complex beneficially alters the pathogenesis of moderate stage idiopathic knee osteoarthritis in male Dunkin Hartley guinea pigs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaryam F Afzali,Lauren B Radakovich,Madeline M Sykes,Margaret A Campbell,Kayley M Patton,Joseph L Sanford,Nicole Vigon,Ryan Ek,Gerardo E Narez,Angela J Marolf,Katie J Sikes,Tammy L Haut Donahue,Kelly S SantangeloPubMed 36578046
- Journal of biomedical science 29:732022LncRNA MRF drives the regulatory function on monocyte recruitment and polarization through HNRNPD-MCP1 axis in mesenchymal stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiajie Lin,Zhongyu Xie,Zhaoqiang Zhang,Ming Li,Guiwen Ye,Wenhui Yu,Jinteng Li,Feng Ye,Zepeng Su,Yunshu Che,Peitao Xu,Chenying Zeng,Peng Wang,Yanfeng Wu,Huiyong ShenPubMed 36127734
- Current issues in molecular biology 44:4303-43132022Myopia Development in Tree Shrew Is Associated with Chronic Inflammatory Reactions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHsiangyu Ku,Jamie Jiin-Yi Chen,Min Hu,Peng-Tai Tien,Hui-Ju Lin,Gezhi Xu,Lei Wan,Dekang GanPubMed 36135208
- Frontiers in cardiovascular medicine 9:9573402022Robust therapeutic effects on COVID-19 of novel small molecules: Alleviation of SARS-CoV-2 S protein induction of ACE2/TMPRSS2, NOX2/ROS, and MCP-1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJi Youn Youn,Jian Wang,Qian Li,Kai Huang,Hua CaiPubMed 36187008
- Immunity, inflammation and disease 10:e6782022SAMD1 attenuates antiphospholipid syndrome-induced vascular injury and pregnancy complications.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRan An,Yanqi Yang,Lei Liu,Peiling LiPubMed 36039649
- Physiological research 71:667-6752022MiR-15a-5p accelerated vascular smooth muscle cells viabilities and migratory abilities via targeting Bcl-2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesH Peng,J Wang,S LiPubMed 36047726
- Cell death & disease 13:1582022Targeting CCL2-CCR4 axis suppress cell migration of head and neck squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZihang Ling,Wei Li,Jiaqi Hu,Yuanyuan Li,Miao Deng,Siyuan Zhang,Xianyue Ren,Tong Wu,Juan Xia,Bin Cheng,Xiaoan TaoPubMed 35177591
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CCL2. The exact immunogen used to generate this antibody is proprietary information. Database link P13500
Reactivity data
Select an application| WB | sELISA | |
|---|---|---|
| Human | Predicted | Predicted |
| Recombinant full length protein - Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 0.1-0.2 µg/mL | Notes To detect hMCAF/MCP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant MCP-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 0.5-2 µg/mL | Notes To detect MCP-1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of ab9669 is required. This antigen affinity purified antibody, in conjunction with ab271250 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant MCP-1. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
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Target data
Function
Acts as a ligand for C-C chemokine receptor CCR2 (PubMed:10529171, PubMed:10587439, PubMed:9837883). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed:10587439, PubMed:9837883). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (PubMed:8195247, PubMed:8627182, PubMed:9792674). May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis (PubMed:8107690).
Alternative names
MCP1, SCYA2, CCL2, C-C motif chemokine 2, HC11, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, Small-inducible cytokine A2, MCAF, MCP-1
Recommended products
Rabbit Polyclonal MCP1 antibody. Suitable for WB, sELISA and reacts with Recombinant full length protein - Human samples. Cited in 103 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CCL2.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CCL2. The exact immunogen used to generate this antibody is proprietary information. Database link P13500
- Purification technique
- Affinity purification Immunogen
- Light chain type
- unknown
- Reconstitution
- Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Although some customers have been successful in IHC we no longer batch test in this application. For and IHC validated antibody please see Anti-MCP1 antibody ab73680
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MCP-1 also known as CCL2 is a chemokine involved in the recruitment of monocytes to sites of injury and inflammation. This protein consists of 76 amino acids and has a molecular weight of about 11 kDa. MCP-1 is expressed in a wide range of cell types including endothelial cells fibroblasts and monocytes. It acts by binding to the chemokine receptor CCR2 stimulating intracellular signaling pathways that mediate cell migration.
- Biological function summary
The role of MCP-1 is significant in guiding immune cells to areas requiring immune response. It does not form part of a complex but operates as a singular entity to affect immune signaling. MCP-1 influences the behavior of monocytes and macrophages by inducing chemotaxis and activating their effector functions. Through this mechanism it aids in the inflammatory response and tissue repair.
- Pathways
MCP-1 participates in the inflammatory pathways by cooperating with key proteins like CCR2. It facilitates the JAK-STAT signaling pathway which is vital for transmitting extracellular information into cellular responses. The interaction of MCP-1 with CCR2 influences the activation of downstream proteins like STAT3 enabling various immune responses. MCP-1 thereby plays a central role in modulating inflammation-related pathways.
- Associated diseases and disorders
MCP-1 has a connection to inflammatory bowel disease and atherosclerosis. In such conditions elevated levels of MCP-1 can exacerbate inflammatory responses by recruiting monocytes to dysfunctional sites worsening tissue damage. Through its interaction with CCR2 MCP-1 becomes a pivotal factor in chronic inflammation. Targeting this MCP-1/CCR2 axis can provide therapeutic opportunities for modulating disease progression in these inflammatory diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-MCP1 antibody (ab9669)
HL-60 cells were incubated at 37°C for 24hrs with vehicle control (0 μM) and varied concentrations of 2-Arachidonylglycerol (ab120098). Increased expression of MCP1 in HL-60 cells correlates with an increase in 2-Arachidonylglycerol concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab9669 at 1 μg/ml and Anti-beta Actin antibody - Loading Control ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution and visualised using ECL development solution.
All lanes: Western blot - Anti-MCP1 antibody (ab9669)
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 11 kDa
Sandwich ELISA - Anti-MCP1 antibody (ab9669)
Standard curve for MCP1 (Analyte: Recombinant human MCP1 protein ab73866); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal to MCP1 (Anti-MCP1 antibody [2.2-4H5-1A11] ab9858) at 1μg/ml and Detector Antibody Rabbit polyclonal to MCP1 (ab9669) at 0.5μg/ml.
Image courtesy of Dr Laura Toma by customer review.Western blot - Anti-MCP1 antibody (ab9669)
MCP1 Western blot staining of lysate prepared from human smooth muscle treated with 100µg/ml glycated LDL using rabbit Anti-MCP1 antibody
All lanes: Western blot - Anti-MCP1 antibody (ab9669) at 1/4000 dilution
All lanes: lysate prepared from human smooth muscle treated with 100µg/ml glycated LDL at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab6721) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 11 kDa
Observed band size: 14-Dec kDa
Exposure time: 1min
Western blot - Anti-MCP1 antibody (ab9669)
All lanes: Western blot - Anti-MCP1 antibody (ab9669) at 0.2 µg/mL
Lane 1: Western blot - Recombinant human MCP1 protein (Recombinant human MCP1 protein ab73866) at 0.01 µg
Lane 2: Western blot - Recombinant human MCP1 protein (Recombinant human MCP1 protein ab73866) at 0.001 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 11 kDa
Exposure time: 1min
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