AB9899

Anti-MCP1 antibody

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(6 Publications)

Rabbit Polyclonal MCP1 antibody. Suitable for sELISA and reacts with Recombinant full length protein - Mouse samples. Cited in 6 publications.

View Alternative Names

Je, Mcp1, Scya2, Ccl2, C-C motif chemokine 2, Monocyte chemoattractant protein 1, Monocyte chemotactic protein 1, Platelet-derived growth factor-inducible protein JE, Small-inducible cytokine A2, MCP-1

1 Images

Sandwich ELISA - Anti-MCP1 antibody (AB9899)

sELISA

Supplier Data

Sandwich ELISA - Anti-MCP1 antibody (AB9899)

ab9899 detecting MCP1 by sandwich ELISA.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

Unknown

Light chain type

unknown

Carrier free

Applications

sELISA

applications

Immunogen

Recombinant Fragment Protein within Mouse Ccl2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P10148

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant full length protein - Mouse": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p>To detect MCP-1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech's Biotinylated Anti-Murine MCP-1 (500-P113Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant MCP-1.</p>" } } }

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Product details

JE, also known as monocyte chemotactic protein (MCP-1), plays an important role in the inflammatory response of blood monocytes and tissue macrophages

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Properties and storage information

Form

Lyophilized

Reconstitution

Reconstitute in 200 µL of water

Purification technique

Affinity purification Immunogen

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCP-1 also known as CCL2 is a chemokine involved in the recruitment of monocytes to sites of injury and inflammation. This protein consists of 76 amino acids and has a molecular weight of about 11 kDa. MCP-1 is expressed in a wide range of cell types including endothelial cells fibroblasts and monocytes. It acts by binding to the chemokine receptor CCR2 stimulating intracellular signaling pathways that mediate cell migration.

Biological function summary

The role of MCP-1 is significant in guiding immune cells to areas requiring immune response. It does not form part of a complex but operates as a singular entity to affect immune signaling. MCP-1 influences the behavior of monocytes and macrophages by inducing chemotaxis and activating their effector functions. Through this mechanism it aids in the inflammatory response and tissue repair.

Pathways

MCP-1 participates in the inflammatory pathways by cooperating with key proteins like CCR2. It facilitates the JAK-STAT signaling pathway which is vital for transmitting extracellular information into cellular responses. The interaction of MCP-1 with CCR2 influences the activation of downstream proteins like STAT3 enabling various immune responses. MCP-1 thereby plays a central role in modulating inflammation-related pathways.

MCP-1 has a connection to inflammatory bowel disease and atherosclerosis. In such conditions elevated levels of MCP-1 can exacerbate inflammatory responses by recruiting monocytes to dysfunctional sites worsening tissue damage. Through its interaction with CCR2 MCP-1 becomes a pivotal factor in chronic inflammation. Targeting this MCP-1/CCR2 axis can provide therapeutic opportunities for modulating disease progression in these inflammatory diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a ligand for C-C chemokine receptor CCR2 (By similarity). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (By similarity). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (By similarity). Plays an important role in mediating peripheral nerve injury-induced neuropathic pain (PubMed : 29993042). Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (PubMed : 29993042).

See full target information Ccl2

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Nephrology (Carlton, Vic.) 27:712-723 PubMed35608936

2022

Peptide 17 alleviates early hypertensive renal injury by regulating the Hippo/YAP signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

San-Bin Xu,Bin Xu,Zhi-Heng Ma,Mei-Qin Huang,Zhi-Sheng Gao,Jian-Li Ni

BMC neuroscience 23:20 PubMed35354428

2022

Cellular distribution of C-C motif chemokine ligand 2 like immunoreactivities in frontal cortex and corpus callosum of normal and lipopolysaccharide treated animal.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Shi,Xinrui Gong,Huangui Xiong,Jingdong Zhang

Cell death & disease 11:513 PubMed32641688

2020

Rab27a dependent exosome releasing participated in albumin handling as a coordinated approach to lysosome in kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Feng,Xin Zhong,Tao-Tao Tang,Cui Wang,Li-Ting Wang,Zuo-Lin Li,Hai-Feng Ni,Bin Wang,Min Wu,Dan Liu,Hong Liu,Ri-Ning Tang,Bi-Cheng Liu,Lin-Li Lv

Frontiers in oncology 10:19 PubMed32064233

2020

Schwann Cell-Derived CCL2 Promotes the Perineural Invasion of Cervical Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Huang,Qiong Fan,Yiwei Wang,Yunxia Cui,Zhihua Wang,Linlin Yang,Xiao Sun,Yudong Wang

Molecular medicine reports 16:8171-8179 PubMed28983592

2017

Amygdalin ameliorates the progression of atherosclerosis in LDL receptor‑deficient mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jianzhen Lv,Wen Xiong,Tiantian Lei,Hailian Wang,Minghan Sun,Erwei Hao,Zhiping Wang,Xiaoqi Huang,Shaoping Deng,Jiagang Deng,Yi Wang

Nature 527:100-104 PubMed26479035

2015

Microenvironment-induced PTEN loss by exosomal microRNA primes brain metastasis outgrowth.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Zhang,Siyuan Zhang,Jun Yao,Frank J Lowery,Qingling Zhang,Wen-Chien Huang,Ping Li,Min Li,Xiao Wang,Chenyu Zhang,Hai Wang,Kenneth Ellis,Mujeeburahiman Cheerathodi,Joseph H McCarty,Diane Palmieri,Jodi Saunus,Sunil Lakhani,Suyun Huang,Aysegul A Sahin,Kenneth D Aldape,Patricia S Steeg,Dihua Yu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-MCP1 antibody

Sandwich ELISA - Anti-MCP1 antibody (AB9899)

Key facts

Host species

Clonality

Isotype

Light chain type

Carrier free

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Reconstitution

Purification technique

Shipped at conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise