Rabbit Polyclonal MCP1 antibody. Suitable for sELISA and reacts with Recombinant full length protein - Mouse samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Ccl2 aa 1 to C-terminus.
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- Nephrology (Carlton, Vic.) 27:712-7232022Peptide 17 alleviates early hypertensive renal injury by regulating the Hippo/YAP signalling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSan-Bin Xu,Bin Xu,Zhi-Heng Ma,Mei-Qin Huang,Zhi-Sheng Gao,Jian-Li NiPubMed 35608936
- BMC neuroscience 23:202022Cellular distribution of C-C motif chemokine ligand 2 like immunoreactivities in frontal cortex and corpus callosum of normal and lipopolysaccharide treated animal.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXue Shi,Xinrui Gong,Huangui Xiong,Jingdong ZhangPubMed 35354428
- Cell death & disease 11:5132020Rab27a dependent exosome releasing participated in albumin handling as a coordinated approach to lysosome in kidney disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYe Feng et. al.PubMed 32641688
- Frontiers in oncology 10:192020Schwann Cell-Derived CCL2 Promotes the Perineural Invasion of Cervical Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing Huang et. al.PubMed 32064233
- Molecular medicine reports 16:8171-81792017Amygdalin ameliorates the progression of atherosclerosis in LDL receptor‑deficient mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianzhen Lv,Wen Xiong,Tiantian Lei,Hailian Wang,Minghan Sun,Erwei Hao,Zhiping Wang,Xiaoqi Huang,Shaoping Deng,Jiagang Deng,Yi WangPubMed 28983592
- Nature 527:100-1042015Microenvironment-induced PTEN loss by exosomal microRNA primes brain metastasis outgrowth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLin Zhang et. al.PubMed 26479035
Key facts
- Isotype
- Unknown
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Mouse Ccl2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P10148
Reactivity data
Select an application| sELISA | |
|---|---|
| Recombinant full length protein - Mouse | Tested |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Mouse | Dilution info - | Notes To detect MCP-1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech's Biotinylated Anti-Murine MCP-1 (500-P113Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant MCP-1. |
Associated Products
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3 products for Alternative Product
Target data
Function
Acts as a ligand for C-C chemokine receptor CCR2 (By similarity). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (By similarity). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (By similarity). Plays an important role in mediating peripheral nerve injury-induced neuropathic pain (PubMed:29993042). Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (PubMed:29993042).
Alternative names
Je, Mcp1, Scya2, Ccl2, C-C motif chemokine 2, Monocyte chemoattractant protein 1, Monocyte chemotactic protein 1, Platelet-derived growth factor-inducible protein JE, Small-inducible cytokine A2, MCP-1
Recommended products
Rabbit Polyclonal MCP1 antibody. Suitable for sELISA and reacts with Recombinant full length protein - Mouse samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Ccl2 aa 1 to C-terminus.
Key facts
- Isotype
- Unknown
- Host species
- Rabbit
- Storage buffer
- Standard buffer
- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Mouse Ccl2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P10148
- Purification technique
- Affinity purification Immunogen
- Light chain type
- unknown
- Reconstitution
- Reconstitute in 200 µL of water
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate long-term storage conditions
- +4°C
Notes
JE, also known as monocyte chemotactic protein (MCP-1), plays an important role in the inflammatory response of blood monocytes and tissue macrophages
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MCP-1 also known as CCL2 is a chemokine involved in the recruitment of monocytes to sites of injury and inflammation. This protein consists of 76 amino acids and has a molecular weight of about 11 kDa. MCP-1 is expressed in a wide range of cell types including endothelial cells fibroblasts and monocytes. It acts by binding to the chemokine receptor CCR2 stimulating intracellular signaling pathways that mediate cell migration.
- Biological function summary
The role of MCP-1 is significant in guiding immune cells to areas requiring immune response. It does not form part of a complex but operates as a singular entity to affect immune signaling. MCP-1 influences the behavior of monocytes and macrophages by inducing chemotaxis and activating their effector functions. Through this mechanism it aids in the inflammatory response and tissue repair.
- Pathways
MCP-1 participates in the inflammatory pathways by cooperating with key proteins like CCR2. It facilitates the JAK-STAT signaling pathway which is vital for transmitting extracellular information into cellular responses. The interaction of MCP-1 with CCR2 influences the activation of downstream proteins like STAT3 enabling various immune responses. MCP-1 thereby plays a central role in modulating inflammation-related pathways.
- Associated diseases and disorders
MCP-1 has a connection to inflammatory bowel disease and atherosclerosis. In such conditions elevated levels of MCP-1 can exacerbate inflammatory responses by recruiting monocytes to dysfunctional sites worsening tissue damage. Through its interaction with CCR2 MCP-1 becomes a pivotal factor in chronic inflammation. Targeting this MCP-1/CCR2 axis can provide therapeutic opportunities for modulating disease progression in these inflammatory diseases.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Sandwich ELISA - Anti-MCP1 antibody (ab9899)
ab9899 detecting MCP1 by sandwich ELISA.
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