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Rabbit Recombinant Multiclonal MCP1 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, IP and reacts with Human, Mouse samples.

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Images

Immunoprecipitation - Anti-MCP1 antibody [RM1100] - BSA and Azide free (AB315479), expandable thumbnail
  • Western blot - Anti-MCP1 antibody [RM1100] - BSA and Azide free (AB315479), expandable thumbnail
  • Western blot - Anti-MCP1 antibody [RM1100] - BSA and Azide free (AB315479), expandable thumbnail
  • Immunoprecipitation - Anti-MCP1 antibody [RM1100] - BSA and Azide free (AB315479), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCP1 antibody [RM1100] - BSA and Azide free (AB315479), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Multiclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)WBIHC-PICC/IFIP
Human
Not recommended
Tested
Tested
Tested
Tested
Mouse
Not recommended
Tested
Tested
Tested
Tested
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Acts as a ligand for C-C chemokine receptor CCR2 (PubMed:10529171, PubMed:10587439, PubMed:9837883). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed:10587439, PubMed:9837883). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (PubMed:8195247, PubMed:8627182, PubMed:9792674). May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis (PubMed:8107690).

Alternative names

MCP1, SCYA2, CCL2, C-C motif chemokine 2, HC11, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, Small-inducible cytokine A2, MCAF, MCP-1

Recommended products

Rabbit Recombinant Multiclonal MCP1 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, IP and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Multiclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
RM1100
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab315479 is the carrier-free version of Anti-MCP1 antibody [RM1100] ab315478.

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MCP-1 also known as CCL2 is a chemokine involved in the recruitment of monocytes to sites of injury and inflammation. This protein consists of 76 amino acids and has a molecular weight of about 11 kDa. MCP-1 is expressed in a wide range of cell types including endothelial cells fibroblasts and monocytes. It acts by binding to the chemokine receptor CCR2 stimulating intracellular signaling pathways that mediate cell migration.

Biological function summary

The role of MCP-1 is significant in guiding immune cells to areas requiring immune response. It does not form part of a complex but operates as a singular entity to affect immune signaling. MCP-1 influences the behavior of monocytes and macrophages by inducing chemotaxis and activating their effector functions. Through this mechanism it aids in the inflammatory response and tissue repair.

Pathways

MCP-1 participates in the inflammatory pathways by cooperating with key proteins like CCR2. It facilitates the JAK-STAT signaling pathway which is vital for transmitting extracellular information into cellular responses. The interaction of MCP-1 with CCR2 influences the activation of downstream proteins like STAT3 enabling various immune responses. MCP-1 thereby plays a central role in modulating inflammation-related pathways.

Associated diseases and disorders

MCP-1 has a connection to inflammatory bowel disease and atherosclerosis. In such conditions elevated levels of MCP-1 can exacerbate inflammatory responses by recruiting monocytes to dysfunctional sites worsening tissue damage. Through its interaction with CCR2 MCP-1 becomes a pivotal factor in chronic inflammation. Targeting this MCP-1/CCR2 axis can provide therapeutic opportunities for modulating disease progression in these inflammatory diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

13 product images

  • Immunoprecipitation - Anti-MCP1 antibody [RM1100] - BSA and Azide free (ab315479), expandable thumbnail

    Immunoprecipitation - Anti-MCP1 antibody [RM1100] - BSA and Azide free (ab315479)

    This data was developed using Anti-MCP1 antibody [RM1100] ab315478, the same antibody clone in a different buffer formulation.

    MCP1 was immunoprecipitated from 0.35 mg RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) treated with 100ng/ml Lipopolysaccharides (LPS) for 7 hours, then 1µg/ml Brefeldin A was added for additional 3 hours whole cell lysate with Anti-MCP1 antibody [RM1100] ab315478 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-MCP1 antibody [RM1100] ab315478 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-MCP1 antibody [RM1100] (Anti-MCP1 antibody [RM1100] ab315478) at 1/1000 dilution

    Lane 1: RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) treated with 100ng/ml Lipopolysaccharides (LPS) for 7 hours, then 1µg/ml Brefeldin A was added for additional 3 hours whole cell lysate

    Lane 2: Anti-MCP1 antibody [RM1100] ab315478 IP in RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) treated with 100ng/ml Lipopolysaccharides (LPS) for 7 hours, then 1µg/ml Brefeldin A was added for additional 3 hours whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-MCP1 antibody [RM1100] ab315478 in RAW 264.7 treated with 100ng/ml Lipopolysaccharides (LPS) for 7 hours, then 1µg/ml Brefeldin A was added for additional 3 hours whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 15s

  • Western blot - Anti-MCP1 antibody [RM1100] - BSA and Azide free (ab315479), expandable thumbnail

    Western blot - Anti-MCP1 antibody [RM1100] - BSA and Azide free (ab315479)

    MCP1 Western blot staining using rabbit Anti-MCP1 antibody

    This data was developed using Anti-MCP1 antibody [RM1100] ab315478, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 28955950).

    Panel A is applied with Anti-MCP1 antibody [RM1100] (Anti-MCP1 antibody [RM1100] ab315478) at 1/1000 dilution and panel B is applied with Anti-MCP1 antibody (Anti-MCP1 antibody ab25124) at 1/1000 dilution.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: 26 seconds (panel A);180 seconds (panel B).

    All lanes: Western blot - Anti-MCP1 antibody [RM1100] (Anti-MCP1 antibody [RM1100] ab315478) at 1/1000 dilution

    Lane 1: Untreated RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Lane 2: RAW 264.7 treated with 100ng/ml Lipopolysaccharides (LPS) for 7 hours, then 1µg/ml Brefeldin A was added for additional 3 hours whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 20 kDa, 36 kDa

  • Western blot - Anti-MCP1 antibody [RM1100] - BSA and Azide free (ab315479), expandable thumbnail

    Western blot - Anti-MCP1 antibody [RM1100] - BSA and Azide free (ab315479)

    MCP1 Western blot staining using rabbit Anti-MCP1 antibody

    This data was developed using Anti-MCP1 antibody [RM1100] ab315478, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-MCP1 antibody [RM1100] (Anti-MCP1 antibody [RM1100] ab315478) at 1/1000 dilution

    Lane 1: Untreated THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

    Lane 2: THP-1 treated with 80nM Phorbol-12-myristate-13-acetate (PMA) for 24 hours, then added 100ng/ml Lipopolysaccharides (LPS) for 7 hours, 1µg/ml Brefeldin A was added for additional 3 hours whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 11 kDa, 36 kDa

    Exposure time: 26s

  • Immunoprecipitation - Anti-MCP1 antibody [RM1100] - BSA and Azide free (ab315479), expandable thumbnail

    Immunoprecipitation - Anti-MCP1 antibody [RM1100] - BSA and Azide free (ab315479)

    This data was developed using Anti-MCP1 antibody [RM1100] ab315478, the same antibody clone in a different buffer formulation.

    MCP1 was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia monocyte) treated with 80nM Phorbol-12-myristate-13-acetate (PMA) for 24 hours, then added 100ng/ml Lipopolysaccharides (LPS) for 7 hours, 1µg/ml Brefeldin A was added for last 3 hours whole cell lysate with Anti-MCP1 antibody [RM1100] ab315478 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-MCP1 antibody [RM1100] ab315478 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-MCP1 antibody [RM1100] (Anti-MCP1 antibody [RM1100] ab315478) at 1/1000 dilution

    Lane 1: THP-1 (human monocytic leukemia monocyte) treated with 80nM Phorbol-12-myristate-13-acetate (PMA) for 24 hours, then added 100ng/ml Lipopolysaccharides (LPS) for 7 hours, 1µg/ml Brefeldin A was added for last 3 hours whole cell lysate

    Lane 2: Anti-MCP1 antibody [RM1100] ab315478 at 1/30 IP in THP-1 (human monocytic leukemia monocyte) treated with 80nM Phorbol-12-myristate-13-acetate (PMA) for 24 hours, then added 100ng/ml Lipopolysaccharides (LPS) for 7 hours, 1µg/ml Brefeldin A was added for last 3 hours whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-MCP1 antibody [RM1100] ab315478 in THP-1 treated with 80nM Phorbol-12-myristate-13-acetate (PMA) for 24 hours, then added 100ng/ml Lipopolysaccharides (LPS) for 7 hours, 1µg/ml Brefeldin A was added for last 3 hours whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 180s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCP1 antibody [RM1100] - BSA and Azide free (ab315479), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCP1 antibody [RM1100] - BSA and Azide free (ab315479)

    This data was developed using Anti-MCP1 antibody [RM1100] ab315478, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded: Image A - RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) cell pellets treated with 100ng/ml Lipopolysaccharides (LPS) for 7 hours, then 1µg/ml Brefeldin A was added for additional 3 hours; Image B - Untreated Raw264.7 cell pellets tissue labeling MCP1 with Anti-MCP1 antibody [RM1100] ab315478 at 1/3000 (0.172 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Cytoplasmic staining on RAW 264.7 cell pellets treated with 100ng/ml Lipopolysaccharides (LPS) for 7 hours, then 1µg/ml Brefeldin A was added for additional 3 hours (Image A); No staining on untreated RAW 264.7 cell pellets (Images B).

    The images on the left are applied with Anti-MCP1 antibody [RM1100] (Anti-MCP1 antibody [RM1100] ab315478) at 1/3000 dilution and the images on the right are applied with Anti-MCP1 antibody (Anti-MCP1 antibody ab25124) at 1/1500 dilution.

    The section was incubated with Anti-MCP1 antibody [RM1100] ab315478 or Anti-MCP1 antibody ab25124 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-MCP1 antibody [RM1100] - BSA and Azide free (ab315479), expandable thumbnail

    Western blot - Anti-MCP1 antibody [RM1100] - BSA and Azide free (ab315479)

    This data was developed using Anti-MCP1 antibody [RM1100] ab315478, the same antibody clone in a different buffer formulation.

    Western blot: Anti-MCP1 antibody (Anti-MCP1 antibody [RM1100] ab315478) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5](Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red.

    In Western blot, Anti-MCP1 antibody [RM1100] ab315478 was shown to bind specifically to MCP1. Target of interest was observed at 11 kDa in wild-type HeLa cell lysates (lane 1/2) with no signal observed at this size in MCP1 knockout cell line (lane 3/4, knockout cell line Human CCL2 (MCP1) knockout HeLa cell line ab255372/knockout cell lysate ab263807). To generate this image, samples were first run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in a fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed in TBS-T, incubated with secondary antibodies Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution for 1 h at room temperature, washed again then imaged.

    All lanes: Western blot - Anti-MCP1 antibody [RM1100] (Anti-MCP1 antibody [RM1100] ab315478) at 1/1000 dilution

    Lane 1: Wild-type HeLa (human cervical adenocarcinoma epithelial cell) treated with 1µg/ml Brefeldin A for 3 hours whole cell lysate at 20 µg with ½ volume of Odyssey Blocking Buffer (TBS)+ ½ volume of TBS

    Lane 2: Wild-type HeLa treated with 1µg/ml TNF-alpha (Recombinant human TNF alpha protein ab9642) for 6 hours, 1µg/ml Brefeldin A was added for additional 3 hours whole cell lysate at 20 µg with ½ volume of Odyssey Blocking Buffer (TBS)+ ½ volume of TBS

    Lane 3: CCL2 knockout HeLa treated with 1µg/ml Brefeldin A for 3 hours whole cell lysate at 20 µg with ½ volume of Odyssey Blocking Buffer (TBS)+ ½ volume of TBS

    Lane 4: CCL2 knockout HeLa treated with TNF-alpha (Recombinant human TNF alpha protein ab9642) for 6 hours, 1µg/ml Brefeldin A was added for additional 3 hours whole cell lysate at 20 µg with ½ volume of Odyssey Blocking Buffer (TBS)+ ½ volume of TBS

    Secondary

    All lanes: Goat Anti-Rabbit IgG H&L (800CW) and Goat Anti-Mouse IgG H&L (680RD) at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 11 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCP1 antibody [RM1100] - BSA and Azide free (ab315479), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCP1 antibody [RM1100] - BSA and Azide free (ab315479)

    This data was developed using Anti-MCP1 antibody [RM1100] ab315478, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling MCP1 with Anti-MCP1 antibody [RM1100] ab315478 at 1/3000 (0.172 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: Anti-MCP1 antibody [RM1100] ab315478 showed no staining on mouse skeletal muscle (ab), while Anti-MCP1 antibody ab25124 showed non-specific staining on the mouse skeletal muscle.

    The image on the left is applied with Anti-MCP1 antibody [RM1100] (Anti-MCP1 antibody [RM1100] ab315478) at 1/3000 dilution and the image on the right is applied with Anti-MCP1 antibody (Anti-MCP1 antibody ab25124) at 1/1500 dilution.

    The section was incubated with Anti-MCP1 antibody [RM1100] ab315478 or Anti-MCP1 antibody ab25124 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCP1 antibody [RM1100] - BSA and Azide free (ab315479), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCP1 antibody [RM1100] - BSA and Azide free (ab315479)

    This data was developed using Anti-MCP1 antibody [RM1100] ab315478, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded: Image A - Mouse lung treated with 1ug/ml Lipopolysaccharides (LPS) and 1ug/ml Brefeldin A for 16 hours; Image B - Untreated mouse lung tissue labeling MCP1 with Anti-MCP1 antibody [RM1100] ab315478 at 1/3000 (0.172 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Cytoplasmic staining on mouse lung treated with 1ug/ml Lipopolysaccharides (LPS) and 1ug/ml Brefeldin A for 16 hours (Image A); nearly no satining on untreated mouse lung (Image B).

    The images on the left are applied with Anti-MCP1 antibody [RM1100] (Anti-MCP1 antibody [RM1100] ab315478) at 1/3000 dilution and the images on the right are applied with Anti-MCP1 antibody (Anti-MCP1 antibody ab25124) at 1/1500 dilution.

    The section was incubated with Anti-MCP1 antibody [RM1100] ab315478 or Anti-MCP1 antibody ab25124 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCP1 antibody [RM1100] - BSA and Azide free (ab315479), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCP1 antibody [RM1100] - BSA and Azide free (ab315479)

    This data was developed using Anti-MCP1 antibody [RM1100] ab315478, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded: Image A - Wild-type Hela cell pellets treated with Brefeldin A (1ug/ml) for 3 hours; Image B - Wild-type Hela cell pellets treated with TNF-alpha (TNFα, 20ng/ml) for 3 hours, 1µg/ml Brefeldin A was added for additional 3 hours; Image C - CCL2 knockout HeLa cell pellets treated with Brefeldin A (1ug/ml) for 3 hours; Image D - CCL2 knockout HeLa cell pellets treated with TNF-alpha (TNFα, 20ng/ml) for 3 hours, 1µg/ml Brefeldin A was added for additional 3 hours tissue labeling MCP1 with Anti-MCP1 antibody [RM1100] ab315478 at 1/3000 (0.172 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on Wild-type Hela cell pellets treated with Brefeldin A (1ug/ml) for 3 hours (Image A) and Wild-type Hela cell pellets treated with TNF-alpha (TNFα, 20ng/ml) for 3 hours, 1µg/ml Brefeldin A was added for additional 3 hours (Image B); No staining on CCL2 knockout HeLa cell pellets treated with Brefeldin A (1ug/ml) for 3 hours (Image C) and CCL2 knockout HeLa cell pellets treated with TNF-alpha (TNFα, 20ng/ml) for 3 hours, 1µg/ml Brefeldin A was added for additional 3 hours (Image D).

    The section was incubated with Anti-MCP1 antibody [RM1100] ab315478 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCP1 antibody [RM1100] - BSA and Azide free (ab315479), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCP1 antibody [RM1100] - BSA and Azide free (ab315479)

    This data was developed using Anti-MCP1 antibody [RM1100] ab315478, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded: Image A - THP-1 (human monocytic leukemia monocyte) cell pellets treated with 80nM Phorbol-12-myristate-13-acetate (PMA) for 24 hours, then added 100ng/ml Lipopolysaccharides (LPS) for 7 hours, 1µg/ml Brefeldin A was added for additional 3 hours; Image B - Untreated THP-1 cell pellets tissue labeling MCP1 with Anti-MCP1 antibody [RM1100] ab315478 at 1/3000 (0.172 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Cytoplasmic staining on THP-1 cell pellets treated with 80nM Phorbol-12-myristate-13-acetate (PMA) for 24 hours, then added 100ng/ml Lipopolysaccharides (LPS) for 7 hours, 1µg/ml Brefeldin A was added for additional 3 hours (Image A); No staining on untreated THP-1 cell pellets (Image B).

    The section was incubated with Anti-MCP1 antibody [RM1100] ab315478 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunocytochemistry/ Immunofluorescence - Anti-MCP1 antibody [RM1100] - BSA and Azide free (ab315479), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MCP1 antibody [RM1100] - BSA and Azide free (ab315479)

    This data was developed using Anti-MCP1 antibody [RM1100] ab315478, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) cells labelling MCP1 with Anti-MCP1 antibody [RM1100] ab315478 at 1/100 (5.17 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

    Confocal image showing cytoplasmic staining on RAW 264.7 cells treated with 100ng/ml Lipopolysaccharides (LPS) for 7 hours, then 1µg/ml Brefeldin A was added for additional 3 hours; no staining on untreated RAW 264.7 cells.

    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-MCP1 antibody [RM1100] - BSA and Azide free (ab315479), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MCP1 antibody [RM1100] - BSA and Azide free (ab315479)

    This data was developed using Anti-MCP1 antibody [RM1100] ab315478, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling MCP1 with Anti-MCP1 antibody [RM1100] ab315478 at 1/100 (5.17 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

    Confocal image showing cytoplasmic staining on THP-1 cells treated with 80nM Phorbol-12-myristate-13-acetate (PMA) for 24 hours, then added 100ng/ml Lipopolysaccharides (LPS) for 7 hours, 1µg/ml Brefeldin A was added for additional 3 hours; no staining on untreated THP-1 cells.

    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCP1 antibody [RM1100] - BSA and Azide free (ab315479), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCP1 antibody [RM1100] - BSA and Azide free (ab315479)

    This data was developed using Anti-MCP1 antibody [RM1100] ab315478, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling MCP1 with Anti-MCP1 antibody [RM1100] ab315478 at 1/3000 (0.172 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on human skeletal muscle.

    The section was incubated with Anti-MCP1 antibody [RM1100] ab315478 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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