JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB277128

Anti-MCP3 antibody [18] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse Monoclonal MCP3 antibody. Carrier free. Suitable for ICC, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CCL7 aa 1 to C-terminus.

View Alternative Names

MCP3, SCYA6, SCYA7, CCL7, C-C motif chemokine 7, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, NC28, Small-inducible cytokine A7, MCP-3

2 Images
Flow Cytometry (Intracellular) - Anti-MCP3 antibody [18] - BSA and Azide free (AB277128)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-MCP3 antibody [18] - BSA and Azide free (AB277128)

Intracellular flow cytometric analysis of CCL7 expression on THP-1 (human monocytic leukemia cell line) cells. The cells were stained with ab277128 at 1/25 dilution, then a FITC-conjugated second step antibody. The Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Immunocytochemistry - Anti-MCP3 antibody [18] - BSA and Azide free (AB277128)
  • ICC

Unknown

Immunocytochemistry - Anti-MCP3 antibody [18] - BSA and Azide free (AB277128)

Immunofluorescence staining of Human CCL7 in SKBR3 (human mammary gland adenocarcinoma cell line) cells. Cells were fixed with 4% PFA, permeabilized with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with ab277128 at 1/60 dilution. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), counterstained with DAPI (blue).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

18

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

ICC, Flow Cyt (Intra)

applications

Immunogen

Recombinant Fragment Protein within Human CCL7 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P80098

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/20 - 1/100", "ICC-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/25 - 1/100", "FlowCytIntra-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target MCP-3 also referred to as CCL7 is a chemotactic cytokine belonging to the CC chemokine family. Its molecular weight is approximately 11 kDa. MCP-3 is largely expressed by various cell types including monocytes lymphocytes and endothelial cells. This protein plays an important role in immune system signaling. MCP-3 exhibits chemotactic activity for monocytes T lymphocytes and natural killer (NK) cells contributing to immune cell recruitment at sites of inflammation.
Biological function summary

MCP-3 interacts with several chemokine receptors such as CCR1 CCR2 and CCR3 allowing for diverse roles in immune response and inflammation. It functions as both a monomer and in conjunction with other chemokines to form heterodimers aiding in the modulation and fine-tuning of the immune response. The presence of MCP-3 in inflammatory tissues indicates its contribution to directing leukocyte migration and positioning therefore supporting both innate and adaptive immunity.

Pathways

MCP-3 is a component of the chemokine signaling pathway and is significant in the regulation of immune surveillance. It interacts closely with proteins such as CCL2 and CCL3 all of which bind to similar receptors like CCR2 and CCR5. MCP-3's involvement in these pathways supports cellular responses to inflammation and infection and its activity has a direct impact on the mobilization and activation of immune cells.

MCP-3 shows associations with conditions marked by inflammation and immune system dysregulation including rheumatoid arthritis and asthma. The protein's influence in these disorders links to other chemokines like MCP-1 (CCL2) which also recruits monocytes and contributes to chronic inflammation. Disruption in MCP-3 expression or function can aggravate disease states by altering the balance of leukocyte trafficking and exacerbating inflammatory responses.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3.
See full target information CCL7
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB193769

Human MCP3 ELISA Kit (CCL7)

SimpleStep

Sandwich ELISA - Human MCP3 ELISA Kit (CCL7) (AB193769)

  • 0
  • 0
Primary Antibodies

AB278327

Anti-CCL1 antibody [EPR24171-79] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-CCL1 antibody [EPR24171-79] - BSA and Azide free (Detector) (AB278327)

  • 0
  • 0
Primary Antibodies

AB242545

Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta antibody [EPR19900-275] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-MIP-1 alpha/CCL3 + CCL4/MIP-1 beta antibody [EPR19900-275] - BSA and Azide free (Capture) (AB242545)

  • 0
  • 0
Primary Antibodies

AB244915

Anti-Amphiregulin antibody [EPR19233-156] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Amphiregulin antibody [EPR19233-156] - BSA and Azide free (Detector) (AB244915)

  • 0
  • 0
Primary Antibodies

AB75853

Anti-beta 2 Microglobulin antibody [EP2978Y]

KO Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-beta 2 Microglobulin antibody [EP2978Y] (AB75853)

  • 4
  • 9

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com