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Rabbit Recombinant Monoclonal MCP3 antibody. Suitable for IP, WB and reacts with Mouse, Human, Transfected cell lysate - Mouse samples. Cited in 4 publications.

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Images

Western blot - Anti-MCP3 antibody [EPR22649-155] (AB228979), expandable thumbnail
  • Western blot - Anti-MCP3 antibody [EPR22649-155] (AB228979), expandable thumbnail
  • Immunoprecipitation - Anti-MCP3 antibody [EPR22649-155] (AB228979), expandable thumbnail
  • Western blot - Anti-MCP3 antibody [EPR22649-155] (AB228979), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Expected
Not recommended
Expected
Not recommended
Not recommended
Mouse
Tested
Not recommended
Tested
Not recommended
Not recommended
Transfected cell lysate - Mouse
Not recommended
Not recommended
Tested
Not recommended
Not recommended

Tested
Tested

Species

Mouse

Dilution info

1/30

Notes

-

Expected
Expected

Species

Human

Dilution info

1/30

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate - Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Transfected cell lysate - Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Transfected cell lysate - Mouse

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Transfected cell lysate - Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Human, Transfected cell lysate - Mouse

Dilution info

-

Notes

-

Target data

Function

Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3.

Alternative names

C-C motif chemokine 7, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, NC28, Small-inducible cytokine A7, MCP-3, CCL7, MCP3, SCYA6, SCYA7

Recommended products

Rabbit Recombinant Monoclonal MCP3 antibody. Suitable for IP, WB and reacts with Mouse, Human, Transfected cell lysate - Mouse samples. Cited in 4 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR22649-155

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The target MCP-3 also referred to as CCL7 is a chemotactic cytokine belonging to the CC chemokine family. Its molecular weight is approximately 11 kDa. MCP-3 is largely expressed by various cell types including monocytes lymphocytes and endothelial cells. This protein plays an important role in immune system signaling. MCP-3 exhibits chemotactic activity for monocytes T lymphocytes and natural killer (NK) cells contributing to immune cell recruitment at sites of inflammation.

Biological function summary

MCP-3 interacts with several chemokine receptors such as CCR1 CCR2 and CCR3 allowing for diverse roles in immune response and inflammation. It functions as both a monomer and in conjunction with other chemokines to form heterodimers aiding in the modulation and fine-tuning of the immune response. The presence of MCP-3 in inflammatory tissues indicates its contribution to directing leukocyte migration and positioning therefore supporting both innate and adaptive immunity.

Pathways

MCP-3 is a component of the chemokine signaling pathway and is significant in the regulation of immune surveillance. It interacts closely with proteins such as CCL2 and CCL3 all of which bind to similar receptors like CCR2 and CCR5. MCP-3's involvement in these pathways supports cellular responses to inflammation and infection and its activity has a direct impact on the mobilization and activation of immune cells.

Associated diseases and disorders

MCP-3 shows associations with conditions marked by inflammation and immune system dysregulation including rheumatoid arthritis and asthma. The protein's influence in these disorders links to other chemokines like MCP-1 (CCL2) which also recruits monocytes and contributes to chronic inflammation. Disruption in MCP-3 expression or function can aggravate disease states by altering the balance of leukocyte trafficking and exacerbating inflammatory responses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-MCP3 antibody [EPR22649-155] (ab228979), expandable thumbnail

    Western blot - Anti-MCP3 antibody [EPR22649-155] (ab228979)

    Lanes 1 - 6: Merged signal (red and green). Green - ab228979 observed at 14 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

    ab228979 was shown to react with MCP3 in RAW 264.7 wild-type cells in Western blot with loss of signal observed in CCL7 knockout sample. Wild-type and CCL7 knockout RAW 264.7 cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab228979 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-MCP3 antibody [EPR22649-155] (ab228979) at 1/1000 dilution

    Lane 1: Wild-type RAW 264.7 untreated control cell lysate at 30 µg

    Lane 2: Wild-type RAW 264.7 PMA treated (80 nM, 24 h) plus LPS treated (100 ng/ml, 6 h) and Brefeldin A (Brefeldin A, Inhibitor of ADP-ribosylation factor ab120299) treated (5 µg/ml, 5 h) cell lysate at 30 µg

    Lane 3: CCL7/MCP3 knockout RAW 264.7 untreated cell lysate at 30 µg

    Lane 4: CCL7/MCP3 knockout RAW 264.7 PMA treated (80 nM, 24 h) plus LPS treated (100 ng/ml, 6 h) and Brefeldin A (Brefeldin A, Inhibitor of ADP-ribosylation factor ab120299) treated (5 µg/ml, 5 h) cell lysate at 30 µg

    Lane 5: J774A.1 Glucose treated (138.8 mMol/L, 8 h) plus Brefeldin A treated (5 µg/ml, 6 h) and Brefeldin A (Brefeldin A, Inhibitor of ADP-ribosylation factor ab120299) treated (5 µg/ml, 5 h) cell lysate at 30 µg

    Lane 6: J774A.1 Glucose treated (5.6 mMol/L, 8 h) plus Brefeldin A treated (5 µg/ml, 6 h) and Brefeldin A (Brefeldin A, Inhibitor of ADP-ribosylation factor ab120299) treated (5 µg/ml, 5 h) cell lysate at 30 µg

    Performed under reducing conditions.

    Predicted band size: 11 kDa

    Observed band size: 14 kDa

  • Western blot - Anti-MCP3 antibody [EPR22649-155] (ab228979), expandable thumbnail

    Western blot - Anti-MCP3 antibody [EPR22649-155] (ab228979)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    All lanes: Western blot - Anti-MCP3 antibody [EPR22649-155] (ab228979) at 1/1000 dilution

    Lane 1: HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 20 µg

    Lane 2: HEK-293T transfected with MCP3 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 11 kDa

    Observed band size: 16-25 kDa

  • Immunoprecipitation - Anti-MCP3 antibody [EPR22649-155] (ab228979), expandable thumbnail

    Immunoprecipitation - Anti-MCP3 antibody [EPR22649-155] (ab228979)

    MCP3 was immunoprecipitated from 0.35 mg RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) (treated with 80 nM Phorbol-12-myristate-13-acetate (PMA) for 24 hours, then with 100 ng/ml lipopolysaccharides (LPS) treated for 6 hours with addition of 1ug/ml of Brefeldin A for the last 3 hours), whole cell lysate with ab228979 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab228979 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution.

    Lane 1: RAW264.7 (treated as above) whole cell lysate 10ug

    Lane 2: ab228979 IP in RAW264.7 (treated as above) whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab228979 in RAW264.7 (treated as above) whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 min.

    All lanes: Immunoprecipitation - Anti-MCP3 antibody [EPR22649-155] (ab228979)

    Predicted band size: 11 kDa

  • Western blot - Anti-MCP3 antibody [EPR22649-155] (ab228979), expandable thumbnail

    Western blot - Anti-MCP3 antibody [EPR22649-155] (ab228979)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    All lanes: Western blot - Anti-MCP3 antibody [EPR22649-155] (ab228979) at 1/1000 dilution

    Lane 1: Untreated RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 40 µg

    Lane 2: RAW264.7 (treated with 80 nM Phorbol-12-myristate-13-acetate (PMA) for 24 hours, then with 100 ng/ml lipopolysaccharides (LPS) treated for 6 hours with addition of 1ug/ml of Brefeldin A for the last 3 hours), whole cell lysate 40 ug.

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 11 kDa

    Observed band size: 11 kDa

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Product protocols

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