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AB251454

Anti-MCP4 antibody [EPR19877] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal MCP4 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

MCP4, NCC1, SCYA13, CCL13, C-C motif chemokine 13, CK-beta-10, Monocyte chemoattractant protein 4, Monocyte chemotactic protein 4, NCC-1, Small-inducible cytokine A13, MCP-4

1 Images
Western blot - Anti-MCP4 antibody [EPR19877] - BSA and Azide free (AB251454)
  • WB

Supplier Data

Western blot - Anti-MCP4 antibody [EPR19877] - BSA and Azide free (AB251454)

This data was developed using ab206405, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The expression profile is consistent with what has been described in the literature (PMID : 9195948).

All lanes:

Western blot - Anti-MCP4 antibody [EPR19877] (<a href='/en-us/products/primary-antibodies/mcp4-antibody-epr19877-ab206405'>ab206405</a>) at 1/1000 dilution

All lanes:

Human small intestine lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 11 kDa

Observed band size: 11 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19877

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab251454 is the carrier-free version of ab206405.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCP4 also known as CCL13 is a chemokine with a molecular mass of approximately 11 kDa. It plays a critical role in attracting immune cells to sites of inflammation. MCP4 achieves this through its interaction with G protein-coupled receptors particularly CCR2 and CCR3. The protein is primarily expressed in tissues involved in immune responses including lymph nodes spleen and bone marrow. It exhibits high expression levels at sites of allergic inflammation suggesting a specific role in these processes.
Biological function summary

MCP4 functions by mediating the recruitment of monocytes eosinophils and T lymphocytes. It is not part of a larger protein complex but rather operates as part of the chemokine signaling family. MCP4 is particularly active in coordinating responses to allergens and pathogens therefore highlighting its importance in the body's defense mechanisms. As a member of the C-C motif chemokine family it shares structural characteristics with other chemokines like CCL11 which participate in similar immune cell trafficking functions.

Pathways

MCP4 is deeply involved in inflammatory and immune pathways specifically the chemokine signaling pathway and the cytokine-cytokine receptor interaction pathway. MCP4 guides immune cells allowing them to respond effectively to cytokine signals ensuring their migration to necessary sites. Through the chemokine signaling pathway it collaborates with related chemokines like CCL5 to enhance immune responses. Its interaction with these pathways highlights its role in both routine immunity and inflammatory responses positioning it as an important player in maintaining immune homeostasis.

MCP4 correlates with conditions such as asthma and allergic rhinitis. Both of these conditions involve chronic inflammation where MCP4's role becomes more pronounced. In asthma the protein contributes to the recruitment of eosinophils and other immune cells that drive the inflammatory processes. MCP4 interactions with proteins like CCR3 are significant in exacerbating symptoms of allergic disorders making it an important target for therapeutic research aimed at modulating inflammatory responses in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens.
See full target information CCL13
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:26521 PubMed39489850

2024

GABPA inhibits tumorigenesis in clear cell renal cell carcinoma by regulating ferroptosis through ACSL4.

Applications

Unspecified application

Species

Unspecified reactive species

Yaqian Wu,Zonglong Wu,Mengfei Yao,Li Liu,Yimeng Song,Lulin Ma,Cheng Liu
View all publications
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Product promise

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