AB121277

Anti-MCPH1 antibody

Name: Anti-MCPH1 antibody (ab121277) | Abcam
Brand: Abcam Limited
SKU: AB121277
Price: 545 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(3 Publications)

Rabbit Polyclonal MCPH1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MCPH1 aa 550-750.

View Alternative Names

Microcephalin, MCPH1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCPH1 antibody (AB121277)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCPH1 antibody (AB121277)

Immunohistochemical analysis of paraffin embedded human testis tissue labeling MCPH1 with ab121277 antibody at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCPH1 antibody (AB121277)

Immunohistochemical analysis of paraffin embedded human cerebellum tissue labeling MCPH1 with ab121277 antibody at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCPH1 antibody (AB121277)

Immunohistochemical analysis of paraffin embedded human liver tissue labeling MCPH1 with ab121277 antibody at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCPH1 antibody (AB121277)

Immunohistochemical analysis of paraffin embedded human cerebral cortex tissue labeling MCPH1 with ab121277 antibody at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-MCPH1 antibody (AB121277)

All lanes:

Western blot - Anti-MCPH1 antibody (ab121277) at 0.4 µg/mL

All lanes:

NTERA-2 (human malignant pluripotent embryonic carcinoma cell line) cell lysate

Predicted band size: 93 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human MCPH1 aa 550-750. The exact immunogen used to generate this antibody is proprietary information.

Q8NEM0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein Microcephalin 1 (MCPH1) also known as BRIT1 plays an important role in cellular processes. It has a molecular mass of approximately 104 kDa. MCPH1 is widely expressed in various tissues with notable expression in the brain and gonads. Its primary mechanical function involves DNA damage response where it helps maintain genome integrity by recruiting repair proteins to sites of damage.

Biological function summary

MCPH1 acts as an important regulator in cell cycle checkpoints particularly the G2/M checkpoint which ensures that cells do not undergo mitosis with damaged DNA. This protein can form complexes with several others to effectively control the replication and repair processes. MCPH1 positively influences chromatin remodeling aiding in both the condensation and decondensation of chromatin structures during different phases of the cell cycle.

Pathways

MCPH1 integrates into both the ATM/ATR and CHK1/CHK2 pathways which are important in DNA damage signaling. These pathways involve the ATM protein which is related to MCPH1 through DNA damage response. In these pathways MCPH1’s regulatory actions are critical for proper AT response and checkpoint activation demonstrating its significance in maintaining genomic stability and cellular function.

MCPH1 mutations have been linked to microcephaly a developmental disorder characterized by reduced brain size. Additionally research associates MCPH1 dysfunction with cancer progression due to its role in maintaining chromosomal stability. The protein p53 connects to MCPH1 through both microcephaly and cancer pathways as it acts in concert with MCPH1 to promote accurate DNA repair and cell cycle regulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Implicated in chromosome condensation and DNA damage induced cellular responses. May play a role in neurogenesis and regulation of the size of the cerebral cortex.

See full target information MCPH1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Medical science monitor : international medical jo 24:7451-7458 PubMed30337515

2018

Silencing BRIT1 Facilitates the Abilities of Invasiveness and Migration in Trophoblast Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Luping Liu,Li Sun,Jing Zheng,Yanchun Wang

PloS one 10:e0144957 PubMed26683461

2015

The UBC Domain Is Required for BRUCE to Promote BRIT1/MCPH1 Function in DSB Signaling and Repair Post Formation of BRUCE-USP8-BRIT1 Complex.

Applications

Unspecified application

Species

Unspecified reactive species

Chunmin Ge,Lixiao Che,Chunying Du

Proceedings of the National Academy of Sciences of the United States of America 112:E1210-9 PubMed25733871

2015

BRUCE regulates DNA double-strand break response by promoting USP8 deubiquitination of BRIT1.

Applications

Unspecified application

Species

Unspecified reactive species

Chunmin Ge,Lixiao Che,Jinyu Ren,Raj K Pandita,Jing Lu,Kaiyi Li,Tej K Pandita,Chunying Du

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MCPH1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCPH1 antibody (AB121277)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCPH1 antibody (AB121277)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCPH1 antibody (AB121277)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCPH1 antibody (AB121277)

Western blot - Anti-MCPH1 antibody (AB121277)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise