Skip to main content

Mouse Monoclonal MCPyV_gp3 large T antigen antibody. Suitable for WB, ICC/IF and reacts with Merkel cell polyomavirus samples. Cited in 5 publications.

Be the first to review this product! Submit a review

Images

Western blot - Anti-MCPyV_gp3 large T antigen antibody [Ab3] (AB202866), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MCPyV_gp3 large T antigen antibody [Ab3] (AB202866), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Merkel cell polyomavirus
Tested
Tested

Tested
Tested

Species
Merkel cell polyomavirus
Dilution info
0.25000-0.50000 µg/mL
Notes

-

Tested
Tested

Species
Merkel cell polyomavirus
Dilution info
5 µg/mL
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Recommended products

Mouse Monoclonal MCPyV_gp3 large T antigen antibody. Suitable for WB, ICC/IF and reacts with Merkel cell polyomavirus samples. Cited in 5 publications.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
Ab3
Purification technique
Affinity purification Protein G
Epitope
The epitope for monoclonal Ab3 is between amino acid residues 79 and 260 of MCPyV large T antigen. Ab3 does not bind to MCPyV small T antigen (PubMed ID: 23114601).
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MCPyV_gp3 large T antigen also known as Merkel cell polyomavirus large T antigen is an important protein involved in the viral replication and transformation processes of Merkel cell polyomavirus (MCPyV). This antigen has an approximate mass of 90 kDa and is primarily expressed in cells infected by MCPyV. The large T antigen is named for its ability to bind to and inactivate tumor suppressor proteins which plays a role in the virus's oncogenic potential.

Biological function summary

The large T antigen serves as a multifunctional protein involved in viral replication cell cycle regulation and DNA damage response. Its function as a viral oncoprotein places it in the heart of tumor development processes. Large T antigen forms part of a complex with host cellular proteins assisting in the disruption of normal cellular controls that prevent tumor formation. This disruption is achieved by binding to cellular tumor suppressors such as p53 and retinoblastoma (Rb) proteins.

Pathways

The large T antigen is involved in cell cycle regulation and tumorigenesis pathways. It plays a substantial role in interfering with the Rb pathway which regulates cell cycle progression. This antigen also impacts the p53 pathway by binding and inactivating the p53 protein preventing apoptosis and contributing to uncontrolled cell proliferation. Both pathways are tightly regulated processes that when dysregulated contribute to oncogenesis.

Associated diseases and disorders

The large T antigen of MCPyV is closely associated with Merkel cell carcinoma a rare but aggressive skin cancer. This cancer frequently arises from the integration of MCPyV DNA into the host genome and the expression of the large T antigen is a critical factor in these cancerous cells. Contacts with host proteins like p53 and Rb underline the antigen's role in the oncogenic processes leading to cancer development. Additionally research suggests potential associations with other disorders related to immune evasion given its role in manipulating host cellular machinery.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-MCPyV_gp3 large T antigen antibody [ab3] (ab202866), expandable thumbnail

    Western blot - Anti-MCPyV_gp3 large T antigen antibody [ab3] (ab202866)

    This blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 5% milk before ab202866 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 0.5ug/ml concentration and 1/20000 dilution respectively. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-MCPyV_gp3 large T antigen antibody [ab3] (ab202866) at 0.5 µg/mL

    Lane 1: MKL-1 whole cell lysate at 20 µg

    Lane 2: HeLa whole cell lysate at 20 µg

    Lane 3: HEK293 whole cell lysate at 20 µg

    Performed under reducing conditions.

    Observed band size: 48 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-MCPyV_gp3 large T antigen antibody [ab3] (ab202866), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MCPyV_gp3 large T antigen antibody [ab3] (ab202866)

    ab202866 staining MCPyV_gp3 large T antigen in MKL-1 cells. The cells were fixed with 4% PFA (10min), permeabilized with 0.1%PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202866 at 5μg/ml and Anti-beta Tubulin antibody - Loading Control ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com