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Rabbit Polyclonal MCT1/Monocarboxylic acid transporter 1 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples. Cited in 12 publications.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFWB
Human
Expected
Expected
Tested
Orangutan
Predicted
Predicted
Predicted

Expected
Expected

Species

Human

Dilution info

5 µg/mL

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species

Orangutan

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

5 µg/mL

Notes

-

Predicted
Predicted

Species

Orangutan

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1 µg/mL

Notes

-

Predicted
Predicted

Species

Orangutan

Dilution info

-

Notes

-

Target data

Function

Proton-coupled monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Depending on the tissue and on cicumstances, mediates the import or export of lactic acid and ketone bodies. Required for normal nutrient assimilation, increase of white adipose tissue and body weight gain when on a high-fat diet. Plays a role in cellular responses to a high-fat diet by modulating the cellular levels of lactate and pyruvate, small molecules that contribute to the regulation of central metabolic pathways and insulin secretion, with concomitant effects on plasma insulin levels and blood glucose homeostasis.

Alternative names

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Rabbit Polyclonal MCT1/Monocarboxylic acid transporter 1 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples. Cited in 12 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Purification technique

Affinity purification Immunogen

Specificity

From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological summary

MCT1 acts as an important transport mechanism for lactate and pyruvate driving the cellular uptake and release processes of these molecules. It partners with CD147 also known as Basigin to form a functional heteromeric complex. This binding allows the correct localization and stabilization of MCT1 in the plasma membrane ensuring effective transport activity. The presence of MCT1 in metabolically active tissues highlights its role in maintaining homeostasis in energy-rich environments.

Mechanical summary

MCT1 also known as Monocarboxylate Transporter 1 or SLC16A1 is a protein that facilitates the transport of monocarboxylates such as lactate and pyruvate across cell membranes. The approximate molecular weight of MCT1 is 51 kDa. This transporter expresses in many tissues including muscle brain liver and kidney. Its widespread presence indicates a significant role in cellular metabolism and energy balance across different biological systems.

Pathway

MCT1 plays a significant role in the glycolysis and gluconeogenesis pathways by mediating lactate transport which connects anaerobic and aerobic metabolic processes. Through these pathways MCT1 associates with key metabolic enzymes like lactate dehydrogenase (LDH) that regulate the conversion between lactate and pyruvate. This association provides a link between lactate production and consumption thereby controlling pH levels and preventing the buildup of metabolic intermediates that can disrupt cellular functions.

Disease

MCT1 is implicated in cancer and metabolic acidosis. In cancer elevated expression of MCT1 supports the Warburg effect a phenomenon where cancer cells rely on aerobic glycolysis leading to increased lactate production. MCT1 works alongside proteins such as CD147 influencing tumor growth and metastasis by promoting lactate export. In metabolic acidosis impaired MCT1 function can disrupt lactate clearance affecting tissue pH balance and contributes to conditions like lactic acidosis. Understanding MCT1's role in these diseases highlights the importance of targeting its function for therapeutic purposes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-MCT1/Monocarboxylic acid transporter 1 antibody (ab85021), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MCT1/Monocarboxylic acid transporter 1 antibody (ab85021)

    ab85021 staining MCT1/Monocarboxylic acid transporter 1 in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab85021 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Also suitable in cells fixed with 4% paraformaldehyde (10 min).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Western blot - Anti-MCT1/Monocarboxylic acid transporter 1 antibody (ab85021), expandable thumbnail

    Western blot - Anti-MCT1/Monocarboxylic acid transporter 1 antibody (ab85021)

    All lanes: Western blot - Anti-MCT1/Monocarboxylic acid transporter 1 antibody (AB85021) at 1 µg/mL

    All lanes: Human skeletal muscle tissue lysate - total protein (ab29330) at 10 µg

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 54 kDa

    Observed band size: 40 kDa, 54 kDa, 89 kDa

    Exposure time: 2min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCT1/Monocarboxylic acid transporter 1 antibody (ab85021), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCT1/Monocarboxylic acid transporter 1 antibody (ab85021)

    IHC image of Monocarboxylic acid transporter 1 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85021, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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