Anti-MCT8 antibody [EPR26257-174] (ab302706)

Rabbit Recombinant Monoclonal MCT 8 antibody. Suitable for IP, Flow Cyt (Intra), ICC/IF, IHC-Fr, IHC-P, WB and reacts with Rat, Mouse, Human samples.

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Images

Western blot - Anti-MCT8 antibody [EPR26257-174] (AB302706), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	Flow Cyt (Intra)	ICC/IF	IHC-Fr	IHC-P	WB
Human	Expected	Expected	Expected	Expected	Tested	Not recommended
Mouse	Tested	Tested	Tested	Tested	Tested	Tested
Rat	Tested	Tested	Tested	Tested	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/30	Notes -
Species Mouse	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/50	Notes -
Species Mouse	Dilution info 1/50	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/500	Notes -
Species Mouse	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/100	Notes -
Species Mouse	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Target data

See full target information SLC16A2

Function

Specific thyroid hormone transmembrane transporter, that mediates both uptake and efflux of thyroid hormones across the cell membrane independently of pH or a Na(+) gradient. Major substrates are the iodothyronines T3 and T4 and to a lesser extent rT3 and 3,3-diiodothyronine (3,3'-T2) (PubMed:16887882, PubMed:18337592, PubMed:20628049, PubMed:23550058, PubMed:26426690, PubMed:27805744, PubMed:31436139). Acts as an important mediator of thyroid hormone transport, especially T3, through the blood-brain barrier (Probable) (PubMed:28526555).

Alternative names

MCT7, MCT8, XPCT, SLC16A2, Monocarboxylate transporter 8, MCT 8, Monocarboxylate transporter 7, Solute carrier family 16 member 2, X-linked PEST-containing transporter, MCT 7

Recommended products

Rabbit Recombinant Monoclonal MCT 8 antibody. Suitable for IP, Flow Cyt (Intra), ICC/IF, IHC-Fr, IHC-P, WB and reacts with Rat, Mouse, Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR26257-174
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MCT 8 also known as monocarboxylate transporter 8 is a protein important for transporting thyroid hormones across cell membranes. This transporter has a mass of approximately 61 kDa. MCT 8 is widely expressed in various tissues including the brain liver and kidneys with a significant presence in neurons. This transporter plays an important mechanical role in hormone regulation by facilitating the cellular uptake and efflux of triiodothyronine (T3) and thyroxine (T4) critical components for proper cellular function.

Biological function summary

MCT 8 serves a significant role in maintaining normal thyroid hormone levels within cells affecting development and metabolism. It does not act alone but operates as part of a cooperative network with other transporters and receptors to ensure precise hormonal balance. Its activity impacts processes like energy generation and usage as well as nervous system development. MCT 8's ability to mediate hormone access to target tissues highlights its importance in these biological processes.

Pathways

MCT 8 is intricately involved in the thyroid hormone signaling pathway. This pathway influences diverse metabolic and developmental outcomes. MCT 8 works closely with other proteins such as thyroid hormone receptor alpha (THRA) that also bind and respond to thyroid hormones. The synchronization between MCT 8 and these proteins ensures effective communication and function within the hormonal axis impacting metabolic regulation and growth.

Associated diseases and disorders

MCT 8 mutations connect strongly to Allan-Herndon-Dudley syndrome a rare neurodevelopmental disorder. This condition arises from disrupted thyroid hormone transport leading to developmental and cognitive deficiencies. Patients typically exhibit reduced T3 levels in the brain. In this context MCT 8's relationship with proteins like THRA reveals how perturbations in hormone transport can cascade into broader physiological abnormalities.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

15 product images

Western blot - Anti-MCT8 antibody [EPR26257-174] (ab302706)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Samples are non-boiled as boiling may cause protein aggregates.
Negative control: spleen (PMID:9545634)
Exposure time: Lane 1: 48 seconds
Lane 2-5: 81 seconds
All lanes: Western blot - Anti-MCT8 antibody [EPR26257-174] (ab302706) at 1/1000 dilution
Lane 1: Mouse kidney tissue lysate 20 µg
Lane 2: Mouse liver tissue lysate 20 µg
Lane 3: Mouse spleen tissue lysate 20 µg
Lane 4: Rat liver tissue lysate 20 µg
Lane 5: Rat spleen tissue lysate 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 60 kDa
Immunohistochemistry (Frozen sections) - Anti-MCT8 antibody [EPR26257-174] (ab302706)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat spleen (fresh) tissue labeling MCT 8 with ab302706 at 1/100 (5.03 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: no staining on rat spleen (PMID: 25409824). is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.<\p>
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Immunohistochemistry (Frozen sections) - Anti-MCT8 antibody [EPR26257-174] (ab302706)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling MCT 8 with ab302706 at 1/100 (5.03 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on rat liver is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.<\p>
.
Flow Cytometry (Intracellular) - Anti-MCT8 antibody [EPR26257-174] (ab302706)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Rat primary neural/glia cell cells labelling MCT 8 with ab302706 at 1/50 dilution (1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
Immunohistochemistry (Frozen sections) - Anti-MCT8 antibody [EPR26257-174] (ab302706)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen (fresh) tissue labeling MCT 8 with ab302706 at 1/100 (5.03 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: no staining on mouse spleen (PMID: 25409824). is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.<\p>
.
Immunohistochemistry (Frozen sections) - Anti-MCT8 antibody [EPR26257-174] (ab302706)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling MCT 8 with ab302706 at 1/100 (5.03 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on mouse liver is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.<\p>
.
Flow Cytometry (Intracellular) - Anti-MCT8 antibody [EPR26257-174] (ab302706)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neural/glia cell cells labelling MCT 8 with ab302706 at 1/50 dilution (1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-MCT8 antibody [EPR26257-174] (ab302706)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling MCT 8 with ab302706 at 1/500 (1.006 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing positive staining in rat primary neuron.Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection is observed. Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Immunocytochemistry/ Immunofluorescence - Anti-MCT8 antibody [EPR26257-174] (ab302706)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling MCT 8 with ab302706 at 1/500 (1.006 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing positive staining in mouse primary neuron.Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection is observed. Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCT8 antibody [EPR26257-174] (ab302706)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling MCT 8 with ab302706 at 1/5000 (0.101 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: no staining in mouse spleen (PMID: 9545634). The section was incubated with ab302706 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCT8 antibody [EPR26257-174] (ab302706)
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling MCT 8 with ab302706 at 1/2000 (0.252 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: no staining in human spleen. The section was incubated with ab302706 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCT8 antibody [EPR26257-174] (ab302706)
Immunohistochemical analysis of paraffin-embedded (A) HEK293T cells co tissue labeling MCT 8 with ab302706 at 1/5000 (0.101 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining in (A) HEK293T cells co-transfected with a MCT8 expression vector containing a his tag and no staining in (B) HEK-293T cells transfected with empty vector containing a his tag. The section was incubated with ab302706 for 30 mins at room. temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCT8 antibody [EPR26257-174] (ab302706)
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling MCT 8 with ab302706 at 1/5000 (0.101 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Membranous staining in rat liver ( (PMID:19641107, PMID:12871948). The section was incubated with ab302706 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCT8 antibody [EPR26257-174] (ab302706)
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling MCT 8 with ab302706 at 1/5000 (0.101 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Membranous staining in mouse kidney (PMID: 19641107, PMID: 19996182). The section was incubated with ab302706 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCT8 antibody [EPR26257-174] (ab302706)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling MCT 8 with ab302706 at 1/2000 (0.252 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Membranous staining in human liver (PMID:19641107, PMID:12871948 ). The section was incubated with ab302706 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins