AB102678

Anti-MCTS1/MCT-1 antibody

Name: Anti-MCTS1/MCT-1 antibody (ab102678) | Abcam
Brand: Abcam Limited
SKU: AB102678

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(2 Publications)

Rabbit Polyclonal MCTS1/MCT-1 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MCTS1 aa 1 to C-terminus.

View Alternative Names

MCT1, MCTS1, Malignant T-cell-amplified sequence 1, MCT-1, Multiple copies T-cell malignancies

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCTS1/MCT-1 antibody (AB102678)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCTS1/MCT-1 antibody (AB102678)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue stained for MCTS1 protein at cytoplasm with ab102678 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCTS1/MCT-1 antibody (AB102678)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue stained for MCTS1 protein at cytoplasm with ab102678 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Unknown

Western blot - Anti-MCTS1/MCT-1 antibody (AB102678)

12% SDS-PAGE

All lanes:

Western blot - Anti-MCTS1/MCT-1 antibody (ab102678) at 1/1000 dilution

All lanes:

Jurkat whole cell lysate at 30 µg

Predicted band size: 21 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MCTS1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9ULC4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCTS1 also known as malignancy-associated gene protein MCT-1 is a protein with a molecular weight of about 18 kDa. It functions as a translation enhancer and is involved in the regulation of protein synthesis. The protein can bind to the eukaryotic translation initiation factor 3 (eIF3) and is expressed in a variety of tissues with higher expression levels often observed in tumor cells. MCTS1's role in promoting efficient translation initiation highlights its mechanistic involvement in the cellular growth process.

Biological function summary

This protein influences cell cycle progression and survival. It forms part of a complex with density-regulated protein (DENR) which aids in ribosomal recycling and the translation initiation of short upstream open reading frames. MCTS1 contributes to the modulation of cellular response to stress and is significant in processes related to cancer cell proliferation.

Pathways

The role of MCTS1 includes involvement in the mTOR and MAPK signaling pathways which regulate cell growth and survival. MCTS1 interacts with the mTOR signaling pathway and can influence translation machinery components linking it to growth-related processes. Additionally its association with eIF3 positions it within the context of translation initiation pathways where it cooperates with other proteins to fine-tune protein synthesis.

The overexpression of MCTS1 links it to cancers such as breast cancer and non-small cell lung cancer. Research indicates that MCTS1 alongside proteins like mTOR and eIF3 play a role in tumor progression and oncogenesis. The protein's involvement in translation initiation and cell growth processes suggests potential as a biomarker and target for therapeutic interventions in these malignancies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Translation regulator forming a complex with DENR to promote translation reinitiation. Translation reinitiation is the process where the small ribosomal subunit remains attached to the mRNA following termination of translation of a regulatory upstream ORF (uORF), and resume scanning on the same mRNA molecule to initiate translation of a downstream ORF, usually the main ORF (mORF). The MCTS1/DENR complex is pivotal to two linked mechanisms essential for translation reinitiation. Firstly, the dissociation of deacylated tRNAs from post-termination 40S ribosomal complexes during ribosome recycling. Secondly, the recruitment in an EIF2-independent manner of aminoacylated initiator tRNA to P site of 40S ribosomes for a new round of translation (PubMed : 16982740, PubMed : 20713520, PubMed : 37875108). This regulatory mechanism governs the translation of more than 150 genes which translation reinitiation is MCTS1/DENR complex-dependent (PubMed : 16982740, PubMed : 20713520, PubMed : 37875108). Consequently, modulates various unrelated biological processes including cell cycle regulation and DNA damage signaling and repair (PubMed : 10440924, PubMed : 11709712, PubMed : 12637315, PubMed : 15897892, PubMed : 16322206, PubMed : 17016429, PubMed : 17416211, PubMed : 9766643). Notably, it positively regulates interferon gamma immunity to mycobacteria by enhancing the translation of JAK2 (PubMed : 37875108).

See full target information MCTS1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 22:531 PubMed34079590

2021

MCTS1 promotes the development of lung adenocarcinoma by regulating E2F1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Cun Gao,Rui Dong,Yongmeng Li,Jinghui Liang,Hui Tian

Proteomics. Clinical applications 8:130-42 PubMed24458570

2014

Aberrant glycosylation in the human trabecular meshwork.

Applications

Unspecified application

Species

Unspecified reactive species

Adam E Sienkiewicz,Brandon N Rosenberg,Genea Edwards,Teresia A Carreon,Sanjoy K Bhattacharya

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-MCTS1/MCT-1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCTS1/MCT-1 antibody (AB102678)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCTS1/MCT-1 antibody (AB102678)

Western blot - Anti-MCTS1/MCT-1 antibody (AB102678)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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