Rabbit Polyclonal MCTS1/MCT-1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MCTS1.
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- Frontiers in genetics 13:8259012022as a Novel Prognostic Biomarker and Its Correlation With Immune Infiltrates in Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMei Deng,Chao Xiong,Zhuo-Kai He,Qiong Bin,Jing-Zhi Song,Wei Li,Jie QinPubMed 35295953
- Translational cancer research 10:1336-13452022High expression of monocarboxylate transporter 4 (MCT 4), but not MCT 1, predicts poor prognosis in patients with non-small cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing-Hui Tong,Xiao-Ping Hu,Xue-Ping Xiang,Luo FangPubMed 35116459
- Cell death discovery 7:572021The oncogene Mct-1 promotes progression of hepatocellular carcinoma via enhancement of Yap-mediated cell proliferation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjie Yang,Yong Ni,Shikun Yang,Yang Ji,Xinchen Yang,Feng Cheng,Xuehao Wang,Feng Zhang,Jianhua RaoPubMed 33753742
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human MCTS1. Database link Q9ULC4
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/500.00000 | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Translation regulator forming a complex with DENR to promote translation reinitiation. Translation reinitiation is the process where the small ribosomal subunit remains attached to the mRNA following termination of translation of a regulatory upstream ORF (uORF), and resume scanning on the same mRNA molecule to initiate translation of a downstream ORF, usually the main ORF (mORF). The MCTS1/DENR complex is pivotal to two linked mechanisms essential for translation reinitiation. Firstly, the dissociation of deacylated tRNAs from post-termination 40S ribosomal complexes during ribosome recycling. Secondly, the recruitment in an EIF2-independent manner of aminoacylated initiator tRNA to P site of 40S ribosomes for a new round of translation (PubMed:16982740, PubMed:20713520, PubMed:37875108). This regulatory mechanism governs the translation of more than 150 genes which translation reinitiation is MCTS1/DENR complex-dependent (PubMed:16982740, PubMed:20713520, PubMed:37875108). Consequently, modulates various unrelated biological processes including cell cycle regulation and DNA damage signaling and repair (PubMed:10440924, PubMed:11709712, PubMed:12637315, PubMed:15897892, PubMed:16322206, PubMed:17016429, PubMed:17416211, PubMed:9766643). Notably, it positively regulates interferon gamma immunity to mycobacteria by enhancing the translation of JAK2 (PubMed:37875108).
Alternative names
MCT1, MCTS1, Malignant T-cell-amplified sequence 1, MCT-1, Multiple copies T-cell malignancies
Recommended products
Rabbit Polyclonal MCTS1/MCT-1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MCTS1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human MCTS1. Database link Q9ULC4
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity greater than 95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MCTS1 also known as malignancy-associated gene protein MCT-1 is a protein with a molecular weight of about 18 kDa. It functions as a translation enhancer and is involved in the regulation of protein synthesis. The protein can bind to the eukaryotic translation initiation factor 3 (eIF3) and is expressed in a variety of tissues with higher expression levels often observed in tumor cells. MCTS1's role in promoting efficient translation initiation highlights its mechanistic involvement in the cellular growth process.
- Biological function summary
This protein influences cell cycle progression and survival. It forms part of a complex with density-regulated protein (DENR) which aids in ribosomal recycling and the translation initiation of short upstream open reading frames. MCTS1 contributes to the modulation of cellular response to stress and is significant in processes related to cancer cell proliferation.
- Pathways
The role of MCTS1 includes involvement in the mTOR and MAPK signaling pathways which regulate cell growth and survival. MCTS1 interacts with the mTOR signaling pathway and can influence translation machinery components linking it to growth-related processes. Additionally its association with eIF3 positions it within the context of translation initiation pathways where it cooperates with other proteins to fine-tune protein synthesis.
- Associated diseases and disorders
The overexpression of MCTS1 links it to cancers such as breast cancer and non-small cell lung cancer. Research indicates that MCTS1 alongside proteins like mTOR and eIF3 play a role in tumor progression and oncogenesis. The protein's involvement in translation initiation and cell growth processes suggests potential as a biomarker and target for therapeutic interventions in these malignancies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-MCTS1/MCT-1 antibody (ab238825)
PC-3 (human prostate adenocarcinoma cell line) cells stained for MCT-1 using ab238825 at a dilution of 1/266 in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCTS1/MCT-1 antibody (ab238825)
Paraffin-embedded human pancreatic cancer tissue stained for MCT-1 with ab238825 at 1/100 dilution in immunohistochemical analysis.
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