AB238825

Anti-MCTS1/MCT-1 antibody

Name: Anti-MCTS1/MCT-1 antibody (ab238825) | Abcam
Brand: Abcam Limited
SKU: AB238825
Price: 510 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal MCTS1/MCT-1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MCTS1.

View Alternative Names

MCT1, MCTS1, Malignant T-cell-amplified sequence 1, MCT-1, Multiple copies T-cell malignancies

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCTS1/MCT-1 antibody (AB238825)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCTS1/MCT-1 antibody (AB238825)

Paraffin-embedded human pancreatic cancer tissue stained for MCT-1 with ab238825 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-MCTS1/MCT-1 antibody (AB238825)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MCTS1/MCT-1 antibody (AB238825)

PC-3 (human prostate adenocarcinoma cell line) cells stained for MCT-1 using ab238825 at a dilution of 1/266 in ICC/IF.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor^®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MCTS1.

Q9ULC4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/500", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity greater than 95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MCTS1 also known as malignancy-associated gene protein MCT-1 is a protein with a molecular weight of about 18 kDa. It functions as a translation enhancer and is involved in the regulation of protein synthesis. The protein can bind to the eukaryotic translation initiation factor 3 (eIF3) and is expressed in a variety of tissues with higher expression levels often observed in tumor cells. MCTS1's role in promoting efficient translation initiation highlights its mechanistic involvement in the cellular growth process.

Biological function summary

This protein influences cell cycle progression and survival. It forms part of a complex with density-regulated protein (DENR) which aids in ribosomal recycling and the translation initiation of short upstream open reading frames. MCTS1 contributes to the modulation of cellular response to stress and is significant in processes related to cancer cell proliferation.

Pathways

The role of MCTS1 includes involvement in the mTOR and MAPK signaling pathways which regulate cell growth and survival. MCTS1 interacts with the mTOR signaling pathway and can influence translation machinery components linking it to growth-related processes. Additionally its association with eIF3 positions it within the context of translation initiation pathways where it cooperates with other proteins to fine-tune protein synthesis.

The overexpression of MCTS1 links it to cancers such as breast cancer and non-small cell lung cancer. Research indicates that MCTS1 alongside proteins like mTOR and eIF3 play a role in tumor progression and oncogenesis. The protein's involvement in translation initiation and cell growth processes suggests potential as a biomarker and target for therapeutic interventions in these malignancies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Translation regulator forming a complex with DENR to promote translation reinitiation. Translation reinitiation is the process where the small ribosomal subunit remains attached to the mRNA following termination of translation of a regulatory upstream ORF (uORF), and resume scanning on the same mRNA molecule to initiate translation of a downstream ORF, usually the main ORF (mORF). The MCTS1/DENR complex is pivotal to two linked mechanisms essential for translation reinitiation. Firstly, the dissociation of deacylated tRNAs from post-termination 40S ribosomal complexes during ribosome recycling. Secondly, the recruitment in an EIF2-independent manner of aminoacylated initiator tRNA to P site of 40S ribosomes for a new round of translation (PubMed : 16982740, PubMed : 20713520, PubMed : 37875108). This regulatory mechanism governs the translation of more than 150 genes which translation reinitiation is MCTS1/DENR complex-dependent (PubMed : 16982740, PubMed : 20713520, PubMed : 37875108). Consequently, modulates various unrelated biological processes including cell cycle regulation and DNA damage signaling and repair (PubMed : 10440924, PubMed : 11709712, PubMed : 12637315, PubMed : 15897892, PubMed : 16322206, PubMed : 17016429, PubMed : 17416211, PubMed : 9766643). Notably, it positively regulates interferon gamma immunity to mycobacteria by enhancing the translation of JAK2 (PubMed : 37875108).

See full target information MCTS1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e27772 PubMed38510035

2024

The oncogene MYBL2 promotes the malignant phenotype and suppresses apoptosis through hedgehog signaling pathway in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjie Yang,Hualin Chen,Lin Ma,Mengchao Wei,Xiaoqiang Xue,Yingjie Li,Zhaoheng Jin,Jie Dong,He Xiao

Frontiers in genetics 13:825901 PubMed35295953

2022

as a Novel Prognostic Biomarker and Its Correlation With Immune Infiltrates in Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Deng,Chao Xiong,Zhuo-Kai He,Qiong Bin,Jing-Zhi Song,Wei Li,Jie Qin

Translational cancer research 10:1336-1345 PubMed35116459

2022

High expression of monocarboxylate transporter 4 (MCT 4), but not MCT 1, predicts poor prognosis in patients with non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ying-Hui Tong,Xiao-Ping Hu,Xue-Ping Xiang,Luo Fang

Oncology letters 22:531 PubMed34079590

2021

MCTS1 promotes the development of lung adenocarcinoma by regulating E2F1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Cun Gao,Rui Dong,Yongmeng Li,Jinghui Liang,Hui Tian

EBioMedicine 66:103301 PubMed33813137

2021

A circular network of coregulated sphingolipids dictates lung cancer growth and progression.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Meng,Xueting Hu,Xinbao Zhao,Xiangzhan Kong,Ya-Ming Meng,Yitian Chen,Liangping Su,Xue Jiang,Xiaoyi Qiu,Cheng Huang,Chao Liu,Minghui Wang,Ping-Pui Wong

Cell death discovery 7:57 PubMed33753742

2021

The oncogene Mct-1 promotes progression of hepatocellular carcinoma via enhancement of Yap-mediated cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjie Yang,Yong Ni,Shikun Yang,Yang Ji,Xinchen Yang,Feng Cheng,Xuehao Wang,Feng Zhang,Jianhua Rao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-MCTS1/MCT-1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCTS1/MCT-1 antibody (AB238825)

Immunocytochemistry/ Immunofluorescence - Anti-MCTS1/MCT-1 antibody (AB238825)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise