AB219827

Anti-MCU antibody [CL3576]

Name: Anti-MCU antibody [CL3576] (ab219827) | Abcam
Brand: Abcam Limited
SKU: AB219827
Price: 500 USD
Availability: InStock
Rating: 3 (3 reviews)

(3 Reviews)

(10 Publications)

Mouse Monoclonal MCU antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MCU aa 250-350.

View Alternative Names

C10orf42, CCDC109A, MCU, HsMCU, Coiled-coil domain-containing protein 109A

2 Images

Supplier Data

Immunocytochemistry - Anti-MCU antibody [CL3576] (AB219827)

PFA-fixed, Triton X-100 permeabilized A549 cells stained for MCU (Green) using ab219827 at 4 μg/ml in ICC/IF.

Microtublues are stained red, nuclei are stained blue.

Supplier Data

Western blot - Anti-MCU antibody [CL3576] (AB219827)

Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative to the control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.

All lanes:

Western blot - Anti-MCU antibody [CL3576] (ab219827) at 1/500 dilution

Lane 1:

A549 transfected with control siRNA cell extract

Lane 2:

A549 transfected with target specific siRNA #1 cell extract

Lane 3:

A549 transfected with target specific siRNA #2 cell extract

Predicted band size: 40 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL3576

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, ICC

applications

Immunogen

Recombinant Fragment Protein within Human MCU aa 250-350. The exact immunogen used to generate this antibody is proprietary information.

Q8NE86

Epitope

Binds to an epitope located within the peptide sequence RDRQYLLFFH as determined by overlapping synthetic peptides.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "2-10 µg/mL", "ICC-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The mitochondrial calcium uniporter (MCU) is an ion channel located in the inner mitochondrial membrane. It functions to facilitate the uptake of calcium ions (Ca2+) into the mitochondria. MCU is sometimes referred to by its alternate names such as mitochondrial calcium uptake1 (MICU1). The molecular weight of MCU is approximately 40 kDa. This protein is expressed in various human tissues with higher levels observed in the heart brain and skeletal muscles.

Biological function summary

The MCU plays a critical role in regulating mitochondrial calcium levels which affect cellular energy production metabolism and apoptosis. MCU is part of a larger protein complex that includes essential regulatory subunits like MICU1 and MICU2. These subunits help maintain precise calcium ion transport into the mitochondria ensuring cellular homeostasis. Under normal conditions MCU controls energy metabolism by regulating ATP production through the mitochondrial electron transport chain.

Pathways

MCU participates in the calcium signaling pathway which links intracellular calcium levels to various metabolic responses. This protein influences the electron transport chain by affecting the activity of enzymes such as FADH2 and NADH which are important in oxidative phosphorylation. Additionally MCU interacts with key regulatory proteins like MICU1 playing a role in adjusting calcium uptake based on the cell’s metabolic needs. These interactions demonstrate MCU's integration with cellular energy pathways and its effect on maintaining metabolic balance.

Researchers have linked dysfunction of MCU to certain neurological disorders such as Parkinson's disease where impaired calcium handling leads to neuronal cell death. Additionally abnormal MCU activity may contribute to the development of cardiac diseases as calcium misregulation affects heart muscle contraction and energy demand. MCU's relationship with these diseases often involves other mitochondrial proteins influencing pathways central to the disease mechanisms. For instance its interaction with MIUC1 and ATP synthase in the mitochondrial pathways has been seen to significantly impact cardiac cellular health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Channel-forming and calcium-conducting subunit of the mitochondrial inner membrane calcium uniporter complex (uniplex), which mediates calcium uptake into the mitochondrial matrix (PubMed : 21685886, PubMed : 21685888, PubMed : 22822213, PubMed : 22829870, PubMed : 22904319, PubMed : 23101630, PubMed : 23178883, PubMed : 23755363, PubMed : 24332854, PubMed : 24560927, PubMed : 26341627, PubMed : 29954988, PubMed : 29995857, PubMed : 30454562, PubMed : 30638448, PubMed : 31080062, PubMed : 32494073, PubMed : 32762847, PubMed : 33296646, PubMed : 37036971, PubMed : 37126688). MCU channel activity is regulated by the calcium-sensor subunits of the uniplex MICU1 and MICU2 (or MICU3) (PubMed : 24560927, PubMed : 26903221, PubMed : 30454562, PubMed : 30638448, PubMed : 32494073, PubMed : 32762847, PubMed : 37036971, PubMed : 37126688). Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates ATP production, cytoplasmic calcium signals and activation of cell death pathways (PubMed : 21685886, PubMed : 21685888, PubMed : 22822213, PubMed : 22829870, PubMed : 22904319, PubMed : 23101630, PubMed : 23178883, PubMed : 23755363, PubMed : 24332854, PubMed : 24560927, PubMed : 26341627, PubMed : 29954988, PubMed : 32494073, PubMed : 32762847). Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes (PubMed : 22822213). While dispensable for baseline homeostatic cardiac function, acts as a key regulator of short-term mitochondrial calcium loading underlying a 'fight-or-flight' response during acute stress : acts by mediating a rapid increase of mitochondrial calcium in pacemaker cells (PubMed : 25603276). Participates in mitochondrial permeability transition during ischemia-reperfusion injury (By similarity). Mitochondrial calcium uptake in skeletal muscle cells is involved in muscle size in adults (By similarity). Regulates synaptic vesicle endocytosis kinetics in central nerve terminal (By similarity). Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake (PubMed : 22829870, PubMed : 22904319). Involved in antigen processing and presentation (By similarity).

See full target information MCU

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 22:485 PubMed39390438

2024

Loss of mitochondrial Ca response and CaMKII/ERK activation by LRRK2 mutation correlate with impaired depolarization-induced mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Eunice Eun-Seo Chang,Huifang Liu,Zoe Yuen-Kiu Choi,Yasine Malki,Steffi Xi-Yue Zhang,Shirley Yin-Yu Pang,Michelle Hiu-Wai Kung,David B Ramsden,Shu-Leong Ho,Philip Wing-Lok Ho

Cell discovery 10:24 PubMed38409220

2024

The E3 ubiquitin ligase MARCH2 protects against myocardial ischemia-reperfusion injury through inhibiting pyroptosis via negative regulation of PGAM5/MAVS/NLRP3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shuolin Liu,Yaguang Bi,Tianting Han,Yiran E Li,Qihang Wang,Ne Natalie Wu,Chenguo Xu,Junbo Ge,Ronggui Hu,Yingmei Zhang

Cardiovascular diabetology 22:216 PubMed37592255

2023

Endothelial MICU1 alleviates diabetic cardiomyopathy by attenuating nitrative stress-mediated cardiac microvascular injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xide Shi,Chao Liu,Jiangwei Chen,Shiqiang Zhou,Yajuan Li,Xingcheng Zhao,Jinliang Xing,Junhui Xue,Fengzhou Liu,Fei Li

Nature communications 14:1595 PubMed36949106

2023

A defect in mitochondrial protein translation influences mitonuclear communication in the heart.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Gao,Tian Liang,Yao Wei Lu,Xuyang Fu,Xiaoxuan Dong,Linbin Pu,Tingting Hong,Yuxia Zhou,Yu Zhang,Ning Liu,Feng Zhang,Jianming Liu,Andrea P Malizia,Hong Yu,Wei Zhu,Douglas B Cowan,Hong Chen,Xinyang Hu,John D Mably,Jian'an Wang,Da-Zhi Wang,Jinghai Chen

Cell death discovery 9:59 PubMed36774369

2023

NMDARs antagonist MK801 suppresses LPS-induced apoptosis and mitochondrial dysfunction by regulating subunits of NMDARs via the CaM/CaMKII/ERK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Min Han,Xiao-Bin Hao,Yi-Xiang Hong,Shan-Shan Zhao,Xu-Chang Chen,Ruiying Wang,Yan Wang,Gang Li

Cell reports 41:111588 PubMed36351382

2022

Pan-claudin family interactome analysis reveals shared and specific interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Lorena Suarez-Artiles,Tilman Breiderhoff,Rossana Girardello,Hannes Gonschior,Sophie Rodius,Antoine Lesur,Ulf Reimer,Evelyn Ramberger,Daniel Perez-Hernandez,Dominik Müller,Philipp Mertins,Gunnar Dittmar

Acta biochimica et biophysica Sinica 54:1528-1539 PubMed36239352

2022

knockdown in hippocampal neurons improves memory performance of an Alzheimer's disease mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyan Cai,Jing Qiao,Siru Chen,Junting Yang,Christian Hölscher,Zhaojun Wang,Jinshun Qi,Meina Wu

Frontiers in microbiology 13:861734 PubMed35359726

2022

Vitamin D3 Suppresses Human Cytomegalovirus-Induced Vascular Endothelial Apoptosis Rectification of Paradoxical m6A Modification of Mitochondrial Calcium Uniporter mRNA, Which Is Regulated by METTL3 and YTHDF3.

Applications

Unspecified application

Species

Unspecified reactive species

Wenbo Zhu,Hongbo Zhang,Shao Wang

Stem cell reports 14:892-908 PubMed32330447

2020

Impairment of Mitochondrial Calcium Buffering Links Mutations in C9ORF72 and TARDBP in iPS-Derived Motor Neurons from Patients with ALS/FTD.

Applications

Unspecified application

Species

Unspecified reactive species

Ruxandra Dafinca,Paola Barbagallo,Lucy Farrimond,Ana Candalija,Jakub Scaber,Nida'a A Ababneh,Chaitra Sathyaprakash,Jane Vowles,Sally A Cowley,Kevin Talbot

Journal of cell science 133: PubMed32051286

2020

Mechanisms of malignancy in glioblastoma cells are linked to mitochondrial Ca uniporter upregulation and higher intracellular Ca levels.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyun Li,Renza Spelat,Anna Bartolini,Daniela Cesselli,Tamara Ius,Miran Skrap,Federica Caponnetto,Ivana Manini,Yili Yang,Vincent Torre

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MCU antibody [CL3576]

Immunocytochemistry - Anti-MCU antibody [CL3576] (AB219827)

Western blot - Anti-MCU antibody [CL3576] (AB219827)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

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