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AB272488

Anti-MCU antibody [EPR24788-158]

  • BOND RX™ Validated
  • 20ul selling size
  • RabMAb
  • Recombinant
  • What is this?

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(9 Publications)

Rabbit Recombinant Monoclonal MCU antibody. Suitable for IP, WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 9 publications.

View Alternative Names

C10orf42, CCDC109A, MCU, HsMCU, Coiled-coil domain-containing protein 109A

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCU antibody [EPR24788-158] (AB272488)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCU antibody [EPR24788-158] (AB272488)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling MCU with ab272488 at 1/500 (1.08 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human colon. The section was incubated with ab272488 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCU antibody [EPR24788-158] (AB272488)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCU antibody [EPR24788-158] (AB272488)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labelling MCU with ab272488 at 1/500 (1.08 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human skeletal muscle. The section was incubated with ab272488 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCU antibody [EPR24788-158] (AB272488)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCU antibody [EPR24788-158] (AB272488)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labelling MCU with ab272488 at 1/500 (1.08 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human cardiac muscle. The section was incubated with ab272488 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Flow Cytometry (Intracellular) - Anti-MCU antibody [EPR24788-158] (AB272488)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MCU antibody [EPR24788-158] (AB272488)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized PANC-1 (Human pancreatic epithelioid carcinoma epithelial cell) cells labelling MCU with ab272488 at 1/50 dilution (1ug)/ (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Immunoprecipitation - Anti-MCU antibody [EPR24788-158] (AB272488)
  • IP

Supplier Data

Immunoprecipitation - Anti-MCU antibody [EPR24788-158] (AB272488)

MCU was immunoprecipitated from 0.35 mg PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate 10 ug with ab272488 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab272488 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate 10 ug

Lane 2 : ab272488 IP in PANC-1 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab272488 in PANC-1 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 8 seconds

All lanes:

Immunoprecipitation - Anti-MCU antibody [EPR24788-158] (ab272488)

Predicted band size: 40 kDa

Observed band size: 30 kDa

false

Western blot - Anti-MCU antibody [EPR24788-158] (AB272488)
  • WB

Lab

Western blot - Anti-MCU antibody [EPR24788-158] (AB272488)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Relatively low expression : human heart (PMID : 31042465).

Exposure time : 8 seconds

All lanes:

Western blot - Anti-MCU antibody [EPR24788-158] (ab272488) at 1/1000 dilution

Lane 1:

Human skeletal muscle tissue lysate at 20 µg

Lane 2:

Human heart tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 40 kDa

Observed band size: 30 kDa

false

Western blot - Anti-MCU antibody [EPR24788-158] (AB272488)
  • WB

Lab

Western blot - Anti-MCU antibody [EPR24788-158] (AB272488)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 48 seconds

All lanes:

Western blot - Anti-MCU antibody [EPR24788-158] (ab272488) at 1/1000 dilution

Lane 1:

PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 40 kDa

Observed band size: 30 kDa

false

  • Carrier free

    Anti-MCU antibody [EPR24788-158] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24788-158

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), WB, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Secondary Antibodies

AB150077

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

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Primary Antibodies

AB320645

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MICU2 antibody [EPR28910-9] - BSA and Azide free (AB320645)

  • 0
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Primary Antibodies

AB14734

Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control

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Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (AB14734)

  • 5
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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The mitochondrial calcium uniporter (MCU) is an ion channel located in the inner mitochondrial membrane. It functions to facilitate the uptake of calcium ions (Ca2+) into the mitochondria. MCU is sometimes referred to by its alternate names such as mitochondrial calcium uptake1 (MICU1). The molecular weight of MCU is approximately 40 kDa. This protein is expressed in various human tissues with higher levels observed in the heart brain and skeletal muscles.
Biological function summary

The MCU plays a critical role in regulating mitochondrial calcium levels which affect cellular energy production metabolism and apoptosis. MCU is part of a larger protein complex that includes essential regulatory subunits like MICU1 and MICU2. These subunits help maintain precise calcium ion transport into the mitochondria ensuring cellular homeostasis. Under normal conditions MCU controls energy metabolism by regulating ATP production through the mitochondrial electron transport chain.

Pathways

MCU participates in the calcium signaling pathway which links intracellular calcium levels to various metabolic responses. This protein influences the electron transport chain by affecting the activity of enzymes such as FADH2 and NADH which are important in oxidative phosphorylation. Additionally MCU interacts with key regulatory proteins like MICU1 playing a role in adjusting calcium uptake based on the cell’s metabolic needs. These interactions demonstrate MCU's integration with cellular energy pathways and its effect on maintaining metabolic balance.

Researchers have linked dysfunction of MCU to certain neurological disorders such as Parkinson's disease where impaired calcium handling leads to neuronal cell death. Additionally abnormal MCU activity may contribute to the development of cardiac diseases as calcium misregulation affects heart muscle contraction and energy demand. MCU's relationship with these diseases often involves other mitochondrial proteins influencing pathways central to the disease mechanisms. For instance its interaction with MIUC1 and ATP synthase in the mitochondrial pathways has been seen to significantly impact cardiac cellular health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Channel-forming and calcium-conducting subunit of the mitochondrial inner membrane calcium uniporter complex (uniplex), which mediates calcium uptake into the mitochondrial matrix (PubMed : 21685886, PubMed : 21685888, PubMed : 22822213, PubMed : 22829870, PubMed : 22904319, PubMed : 23101630, PubMed : 23178883, PubMed : 23755363, PubMed : 24332854, PubMed : 24560927, PubMed : 26341627, PubMed : 29954988, PubMed : 29995857, PubMed : 30454562, PubMed : 30638448, PubMed : 31080062, PubMed : 32494073, PubMed : 32762847, PubMed : 33296646, PubMed : 37036971, PubMed : 37126688). MCU channel activity is regulated by the calcium-sensor subunits of the uniplex MICU1 and MICU2 (or MICU3) (PubMed : 24560927, PubMed : 26903221, PubMed : 30454562, PubMed : 30638448, PubMed : 32494073, PubMed : 32762847, PubMed : 37036971, PubMed : 37126688). Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates ATP production, cytoplasmic calcium signals and activation of cell death pathways (PubMed : 21685886, PubMed : 21685888, PubMed : 22822213, PubMed : 22829870, PubMed : 22904319, PubMed : 23101630, PubMed : 23178883, PubMed : 23755363, PubMed : 24332854, PubMed : 24560927, PubMed : 26341627, PubMed : 29954988, PubMed : 32494073, PubMed : 32762847). Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes (PubMed : 22822213). While dispensable for baseline homeostatic cardiac function, acts as a key regulator of short-term mitochondrial calcium loading underlying a 'fight-or-flight' response during acute stress : acts by mediating a rapid increase of mitochondrial calcium in pacemaker cells (PubMed : 25603276). Participates in mitochondrial permeability transition during ischemia-reperfusion injury (By similarity). Mitochondrial calcium uptake in skeletal muscle cells is involved in muscle size in adults (By similarity). Regulates synaptic vesicle endocytosis kinetics in central nerve terminal (By similarity). Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake (PubMed : 22829870, PubMed : 22904319). Involved in antigen processing and presentation (By similarity).
See full target information MCU
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 31:42 PubMed39905300

2025

TAB2 deficiency induces dilated cardiomyopathy by promoting mitochondrial calcium overload in human iPSC-derived cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Wenrui Sun,Jianchao Zhang,Shuang Li,Wanrong Fu,Yangyang Liu,Mengduan Liu,Jianzeng Dong,Xiaoyan Zhao,Xiaowei Li

Cell biology and toxicology 41:21 PubMed39753834

2025

Identification of cancer-associated fibroblast subtypes and prognostic model development in breast cancer: role of the RUNX1/SDC1 axis in promoting invasion and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Yunhao Wu,Nu Li,Jin Shang,Jiazi Jiang,Xiaoliang Liu

Cell communication and signaling : CCS 22:464 PubMed39350150

2024

ER-mitochondria contact sites regulate hepatic lipogenesis via Ip3r-Grp75-Vdac complex recruiting Seipin.

Applications

Unspecified application

Species

Unspecified reactive species

Ying-Jia Chi,Zhen-Yu Bai,Guang-Li Feng,Xiao-Hong Lai,Yu-Feng Song

Frontiers in oncology 14:1355559 PubMed38737905

2024

The clinical significance of mitochondrial calcium uniporter in gastric cancer patients and its preliminary exploration of the impact on mitochondrial function and metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Zipeng Xu,Xia Chen,Haicun Zhou,Luming Sun,Ruobing Bai,Wenwen Yu,Junhao Yang,Hongbin Liu

The Journal of cell biology 222: PubMed37676315

2023

C17orf80 binds the mitochondrial genome to promote its replication.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Wu,Wenshuo Zhang,Fengli Xu,Kun Peng,Xiaoyu Liu,Wanqiu Ding,Qi Ma,Heping Cheng,Xianhua Wang

Asia-Pacific journal of clinical oncology 19:e248-e257 PubMed36271660

2022

Polysaccharide of Lactobacillus casei SB27 reduced colon cancer cell prognosis through mitochondrial damage by upregulation of HINT2.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Di,Xin Li,Qiaoyi Yang

Oxidative medicine and cellular longevity 2022:7727006 PubMed36148414

2022

MCU Inhibitor Ruthenium Red Alleviates the Osteoclastogenesis and Ovariectomized Osteoporosis via Suppressing RANKL-Induced ROS Production and NFATc1 Activation through P38 MAPK Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxin Wang,Xiang Li,Shengji Zhou,Jiarui Li,Yi Zhu,Quan Wang,Fengchao Zhao

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e22468 PubMed35913801

2022

Bile acid restrained T cell activation explains cholestasis aggravated hepatitis B virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Chujie Ding,Yu Hong,Yuan Che,Tianyu He,Yun Wang,Shule Zhang,Jiawei Wu,Wanfeng Xu,Jingyi Hou,Haiping Hao,Lijuan Cao

Experimental and therapeutic medicine 23:426 PubMed35607377

2022

Effect of ginkgolide K on calcium channel activity in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Hongbin Liu,Qinyun Li,Xiaodan Zhang,Yun Shi,Jinyi Li
View all publications
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