Rabbit Recombinant Monoclonal MCU antibody. Carrier free. Suitable for IP, WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (AB282592), expandable thumbnail](https://content.abcam.com/products/images/mcu-antibody-epr24788-158-bsa-and-azide-free-ab282592--western-blot-img164590.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (AB282592)")
![Western blot - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (AB282592), expandable thumbnail](https://content.abcam.com/products/images/mcu-antibody-epr24788-158-bsa-and-azide-free-ab282592--western-blot-img169792.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (AB282592)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (AB282592), expandable thumbnail](https://content.abcam.com/products/images/mcu-antibody-epr24788-158-bsa-and-azide-free-ab282592--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img175038.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (AB282592)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (AB282592), expandable thumbnail](https://content.abcam.com/products/images/mcu-antibody-epr24788-158-bsa-and-azide-free-ab282592--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img174182.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (AB282592)")
![Immunoprecipitation - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (AB282592), expandable thumbnail](https://content.abcam.com/products/images/mcu-antibody-epr24788-158-bsa-and-azide-free-ab282592--immunoprecipitation-img182308.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (AB282592)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Channel-forming and calcium-conducting subunit of the mitochondrial inner membrane calcium uniporter complex (uniplex), which mediates calcium uptake into the mitochondrial matrix (PubMed:21685886, PubMed:21685888, PubMed:22822213, PubMed:22829870, PubMed:22904319, PubMed:23101630, PubMed:23178883, PubMed:23755363, PubMed:24332854, PubMed:24560927, PubMed:26341627, PubMed:29954988, PubMed:29995857, PubMed:30454562, PubMed:30638448, PubMed:31080062, PubMed:32494073, PubMed:32762847, PubMed:33296646, PubMed:37036971, PubMed:37126688). MCU channel activity is regulated by the calcium-sensor subunits of the uniplex MICU1 and MICU2 (or MICU3) (PubMed:24560927, PubMed:26903221, PubMed:30454562, PubMed:30638448, PubMed:32494073, PubMed:32762847, PubMed:37036971, PubMed:37126688). Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates ATP production, cytoplasmic calcium signals and activation of cell death pathways (PubMed:21685886, PubMed:21685888, PubMed:22822213, PubMed:22829870, PubMed:22904319, PubMed:23101630, PubMed:23178883, PubMed:23755363, PubMed:24332854, PubMed:24560927, PubMed:26341627, PubMed:29954988, PubMed:32494073, PubMed:32762847). Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes (PubMed:22822213). While dispensable for baseline homeostatic cardiac function, acts as a key regulator of short-term mitochondrial calcium loading underlying a 'fight-or-flight' response during acute stress: acts by mediating a rapid increase of mitochondrial calcium in pacemaker cells (PubMed:25603276). Participates in mitochondrial permeability transition during ischemia-reperfusion injury (By similarity). Mitochondrial calcium uptake in skeletal muscle cells is involved in muscle size in adults (By similarity). Regulates synaptic vesicle endocytosis kinetics in central nerve terminal (By similarity). Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake (PubMed:22829870, PubMed:22904319). Involved in antigen processing and presentation (By similarity).
Alternative names
C10orf42, CCDC109A, MCU, HsMCU, Coiled-coil domain-containing protein 109A
Recommended products
Rabbit Recombinant Monoclonal MCU antibody. Carrier free. Suitable for IP, WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR24788-158
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab282592 is the carrier-free version of Anti-MCU antibody [EPR24788-158] ab272488.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The mitochondrial calcium uniporter (MCU) is an ion channel located in the inner mitochondrial membrane. It functions to facilitate the uptake of calcium ions (Ca2+) into the mitochondria. MCU is sometimes referred to by its alternate names such as mitochondrial calcium uptake1 (MICU1). The molecular weight of MCU is approximately 40 kDa. This protein is expressed in various human tissues with higher levels observed in the heart brain and skeletal muscles.
- Biological function summary
The MCU plays a critical role in regulating mitochondrial calcium levels which affect cellular energy production metabolism and apoptosis. MCU is part of a larger protein complex that includes essential regulatory subunits like MICU1 and MICU2. These subunits help maintain precise calcium ion transport into the mitochondria ensuring cellular homeostasis. Under normal conditions MCU controls energy metabolism by regulating ATP production through the mitochondrial electron transport chain.
- Pathways
Four words that describe them include calcium signaling and energy metabolism. MCU participates in the calcium signaling pathway which links intracellular calcium levels to various metabolic responses. This protein influences the electron transport chain by affecting the activity of enzymes such as FADH2 and NADH which are important in oxidative phosphorylation. Additionally MCU interacts with key regulatory proteins like MICU1 playing a role in adjusting calcium uptake based on the cell’s metabolic needs. These interactions demonstrate MCU's integration with cellular energy pathways and its effect on maintaining metabolic balance.
- Associated diseases and disorders
Four words that affect them include neurodegeneration and cardiac disease. Researchers have linked dysfunction of MCU to certain neurological disorders such as Parkinson's disease where impaired calcium handling leads to neuronal cell death. Additionally abnormal MCU activity may contribute to the development of cardiac diseases as calcium misregulation affects heart muscle contraction and energy demand. MCU's relationship with these diseases often involves other mitochondrial proteins influencing pathways central to the disease mechanisms. For instance its interaction with MIUC1 and ATP synthase in the mitochondrial pathways has been seen to significantly impact cardiac cellular health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (ab282592), expandable thumbnail](https://content.abcam.com/products/images/mcu-antibody-epr24788-158-bsa-and-azide-free-ab282592--western-blot-img164590.jpg "Western blot - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (ab282592)")
Western blot - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (ab282592)
This data was developed using 272488, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Relatively low expression: human heart (PMID: 31042465).
Exposure time: 8 seconds
All lanes: Western blot - Anti-MCU antibody [EPR24788-158] (Anti-MCU antibody [EPR24788-158] ab272488) at 1/1000 dilution
Lane 1: Human skeletal muscle tissue lysate at 20 µg
Lane 2: Human heart tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 40 kDa
Observed band size: 30 kDa
![Western blot - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (ab282592), expandable thumbnail](https://content.abcam.com/products/images/mcu-antibody-epr24788-158-bsa-and-azide-free-ab282592--western-blot-img169792.jpg "Western blot - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (ab282592)")
Western blot - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (ab282592)
This data was developed using 272488, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 48 seconds
All lanes: Western blot - Anti-MCU antibody [EPR24788-158] (Anti-MCU antibody [EPR24788-158] ab272488) at 1/1000 dilution
Lane 1: PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 40 kDa
Observed band size: 30 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (ab282592), expandable thumbnail](https://content.abcam.com/products/images/mcu-antibody-epr24788-158-bsa-and-azide-free-ab282592--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img175038.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (ab282592)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (ab282592)
This data was developed using Anti-MCU antibody [EPR24788-158] ab272488, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling MCU with Anti-MCU antibody [EPR24788-158] ab272488 at 1/500 (1.08 ug/ml) dilution followed by a Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) at Ready to use dilution. Positive staining on human colon. The section was incubated with Anti-MCU antibody [EPR24788-158] ab272488 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) at Ready to use dilution.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (ab282592), expandable thumbnail](https://content.abcam.com/products/images/mcu-antibody-epr24788-158-bsa-and-azide-free-ab282592--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img174182.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (ab282592)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (ab282592)
This data was developed using Anti-MCU antibody [EPR24788-158] ab272488, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling MCU with Anti-MCU antibody [EPR24788-158] ab272488 at 1/500 (1.08 ug/ml) dilution followed by a Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) at Ready to use dilution. Cytoplasmic staining on human skeletal muscle. The section was incubated with Anti-MCU antibody [EPR24788-158] ab272488 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) at Ready to use dilution.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Immunoprecipitation - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (ab282592), expandable thumbnail](https://content.abcam.com/products/images/mcu-antibody-epr24788-158-bsa-and-azide-free-ab282592--immunoprecipitation-img182308.jpg "Immunoprecipitation - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (ab282592)")
Immunoprecipitation - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (ab282592)
This data was developed using Anti-MCU antibody [EPR24788-158] ab272488, the same antibody clone in a different buffer formulation.
MCU was immunoprecipitated from 0.35 mg PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate 10 ug with Anti-MCU antibody [EPR24788-158] ab272488 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-MCU antibody [EPR24788-158] ab272488 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: PANC-1 (human pancreatic epithelioid carcinoma epithelial cell) whole cell lysate 10 ug
Lane 2: Anti-MCU antibody [EPR24788-158] ab272488 IP in PANC-1 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-MCU antibody [EPR24788-158] ab272488 in PANC-1 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 8 seconds
All lanes: Immunoprecipitation - Anti-MCU antibody [EPR24788-158] (Anti-MCU antibody [EPR24788-158] ab272488)
Predicted band size: 40 kDa
Observed band size: 30 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (ab282592), expandable thumbnail](https://content.abcam.com/products/images/mcu-antibody-epr24788-158-bsa-and-azide-free-ab282592--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img169287.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (ab282592)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (ab282592)
This data was developed using Anti-MCU antibody [EPR24788-158] ab272488, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling MCU with Anti-MCU antibody [EPR24788-158] ab272488 at 1/500 (1.08 ug/ml) dilution followed by a Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) at Ready to use dilution. Cytoplasmic staining on human cardiac muscle. The section was incubated with Anti-MCU antibody [EPR24788-158] ab272488 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) at Ready to use dilution.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Flow Cytometry (Intracellular) - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (ab282592), expandable thumbnail](https://content.abcam.com/products/images/mcu-antibody-epr24788-158-bsa-and-azide-free-ab282592--flow-cytometry-intracellular-img164418.jpg "Flow Cytometry (Intracellular) - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (ab282592)")
Flow Cytometry (Intracellular) - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (ab282592)
This data was developed using Anti-MCU antibody [EPR24788-158] ab272488, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized PANC-1 (Human pancreatic epithelioid carcinoma epithelial cell) cells labelling MCU with Anti-MCU antibody [EPR24788-158] ab272488 at 1/50 dilution (1ug)/ (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (ab282592), expandable thumbnail](https://content.abcam.com/products/images/mcu-antibody-epr24788-158-bsa-and-azide-free-ab282592--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img169287.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (ab282592)")
![Flow Cytometry (Intracellular) - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (ab282592), expandable thumbnail](https://content.abcam.com/products/images/mcu-antibody-epr24788-158-bsa-and-azide-free-ab282592--flow-cytometry-intracellular-img164418.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-MCU antibody [EPR24788-158] - BSA and Azide free (ab282592)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com