Anti-MD2 antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MD2 antibody (AB24182)

Immunofluorescent analysis of MD2 in human spleen cells with ab24182 at 20 μg/mL in ICC/IF.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MD2 antibody (AB24182)

Immunohistochemical analysis of human spleen tissue stained for MD2 using ab24182 at 2.5μg/mL.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MD2 antibody (AB24182)

ab24182 at 2μg/ml staining of MD2 in rat spleen cells by IHC

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MD2 antibody (AB24182)

Immunofluorescent analysis of MD2 in Rat Spleen cells with ab24182 at 10 μg/mL in ICC/IF.

• WB

Supplier Data

Western blot - Anti-MD2 antibody (AB24182)

WB Validation with Human MD-2 Overexpressed Lysate.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-beta-actin antibody - Loading Control.

All lanes:

Western blot - Anti-MD2 antibody (ab24182) at 2 µg/mL

Lane 1:

Wild-type (WT) expression lysate at 10 µg

Lane 2:

Overexpressed (OVE) Lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugated at 1/10000 dilution

Predicted band size: 18 kDa

false

Exposure time: 2min

• WB

Supplier Data

Western blot - Anti-MD2 antibody (AB24182)

Lane 1:

Western blot - Anti-MD2 antibody (ab24182) at 0.5 µg/mL

Lane 2:

Western blot - Anti-MD2 antibody (ab24182) at 1 µg/mL

All lanes:

Mouse spleen tissue at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 18 kDa

false

• WB

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Western blot - Anti-MD2 antibody (AB24182)

Blocking Step : 5% Milk for 1 hour at room temperature.

All lanes:

Western blot - Anti-MD2 antibody (ab24182) at 2 µg/mL

Lane 1:

Whole cell lysate of rat cortical astrocytes treated with control siRNA for 96 hours at 40 µg

Lane 2:

Whole cell lysate of rat cortical astrocytes treated with control siRNA for 96 hours and LPS (50 ng/ml) for 10 minutes at 40 µg

Lane 3:

Whole cell lysate of rat cortical astrocytes treated with control siRNA for 96 hours and ethanol (50 mM) for 10 minutes at 40 µg

Lane 4:

Whole cell lysate of rat cortical astrocytes treated with MD2 siRNA for 96 hours at 40 µg

Lane 5:

Whole cell lysate of rat cortical astrocytes treated with MD2 siRNA for 96 hours and LPS (50 ng/ml) for 10 minutes at 40 µg

Lane 6:

Whole cell lysate of rat cortical astrocytes treated with MD2 siRNA for 96 hours and ethanol (50 mM) for 10 minutes at 40 µg

Secondary

All lanes:

An HRP-conjugated Goat anti-rabbit IgG at 1/20000 dilution

Predicted band size: 18 kDa

Observed band size: 25 kDa

true

Exposure time: 2min

This image is courtesy of an Abreview submitted by C. Guerri

• WB

Supplier Data

Western blot - Anti-MD2 antibody (AB24182)

All lanes:

Western blot - Anti-MD2 antibody (ab24182)

All lanes:

Rat spleen lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 18 kDa

false

• WB

Supplier Data

Western blot - Anti-MD2 antibody (AB24182)

All lanes:

Western blot - Anti-MD2 antibody (ab24182) at 1 µg/mL

All lanes:

Daudi cell lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 18 kDa

false

• WB

Supplier Data

Western blot - Anti-MD2 antibody (AB24182)

All lanes:

Western blot - Anti-MD2 antibody (ab24182) at 1 µg/mL

Predicted band size: 18 kDa

Observed band size: 26 kDa

false

• WB

Supplier Data

Western blot - Anti-MD2 antibody (AB24182)

All lanes:

Western blot - Anti-MD2 antibody (ab24182) at 1 µg/mL

Lane 1:

Mouse spleen lysate at 15 µg

Lane 2:

Mouse thymus lysate at 15 µg

Lane 3:

Mouse testis lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 18 kDa

false