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AB79055

Anti-MDA5 antibody

4

(1 Review)

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(13 Publications)

Rabbit Polyclonal MDA5 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human IFIH1.

View Alternative Names

MDA5, RH116, IFIH1, Interferon-induced helicase C domain-containing protein 1, Clinically amyopathic dermatomyositis autoantigen 140 kDa, Helicase with 2 CARD domains, Interferon-induced with helicase C domain protein 1, Melanoma differentiation-associated protein 5, Murabutide down-regulated protein, RIG-I-like receptor 2, RNA helicase-DEAD box protein 116, CADM-140 autoantigen, Helicard, MDA-5, RLR-2

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDA5 antibody (AB79055)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDA5 antibody (AB79055)

Immunohistochemical analysis of MDA5 expression in paraffin embedded, formalin fixed human pancreas using 5 µg/ml ab79055

Immunocytochemistry/ Immunofluorescence - Anti-MDA5 antibody (AB79055)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MDA5 antibody (AB79055)

ICC/IF image of ab79055 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79055, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDA5 antibody (AB79055)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDA5 antibody (AB79055)

Immunohistochemical analysis of MDA5 expression in paraffin embedded, formalin fixed mouse kidney using 5 μg/ml ab79055

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDA5 antibody (AB79055)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDA5 antibody (AB79055)

Immunohistochemical analysis of MDA5 expression in paraffin embedded, formalin fixed mouse pancreas using 5 μg/ml ab79055

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDA5 antibody (AB79055)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDA5 antibody (AB79055)

Immunohistochemical analysis of MDA5 expression in paraffin embedded, formalin fixed mouse small intestine using 5 μg/ml ab79055

Western blot - Anti-MDA5 antibody (AB79055)
  • WB

Supplier Data

Western blot - Anti-MDA5 antibody (AB79055)

Lane 1:

Western blot - Anti-MDA5 antibody (ab79055) at 1 µg/mL

Lane 2:

Western blot - Anti-MDA5 antibody (ab79055) at 2 µg/mL

Lane 3:

Western blot - Anti-MDA5 antibody (ab79055) at 4 µg/mL

All lanes:

Daudi cell lysate

Predicted band size: 117 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human IFIH1. The exact immunogen used to generate this antibody is proprietary information.

Q9BYX4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-4 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MDA5 also known as melanoma differentiation-associated protein 5 or IFIH1 is a cytosolic sensor involved in the detection of viral RNA. It is a member of the RIG-I-like receptor family and possesses a molecular weight of approximately 116 kilodaltons. MDA5 protein is expressed across various tissues but shows higher expression in the immune system's cells including macrophages and dendritic cells. MDA5 recognizes long double-stranded RNA a common viral replication byproduct triggering immune responses.
Biological function summary

In cellular defense mechanisms MDA5 plays an important role in initiating antiviral responses. It operates as part of a signaling complex that includes MAVS (mitochondrial antiviral-signaling protein) leading to the production of type I interferons and pro-inflammatory cytokines. These responses promote an antiviral state in host cells aiding in the containment and clearance of viral infections. MDA5 antibodies can be useful tools for studying these immune processes.

Pathways

MDA5 interacts with antiviral signaling pathways such as the IFN signaling pathway and the RIG-I-like receptor signaling pathway. Upon recognizing viral RNA MDA-5 cooperates with proteins like RIG-I and MAVS to activate downstream signaling cascades that mediate immune responses. This collaboration enables efficient viral recognition and response limiting viral replication and spread.

MDA5 has associations with autoimmune diseases like systemic lupus erythematosus and certain types of dermatomyositis. These conditions often involve autoantibodies against MDA5 indicating a possible role in pathogenesis. In these diseases altered function or regulation of related proteins including IFN-induced proteins may contribute to chronic inflammation and autoimmunity. Understanding MDA5's involvement in these contexts is essential for developing therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and pro-inflammatory cytokines (PubMed : 28594402, PubMed : 32169843, PubMed : 33727702). Its ligands include mRNA lacking 2'-O-methylation at their 5' cap and long-dsRNA (>1 kb in length) (PubMed : 22160685). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases : TBK1 and IKBKE which phosphorylate interferon regulatory factors : IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV), mengo encephalomyocarditis virus (ENMG), and rhinovirus (PubMed : 28606988). Detects coronavirus SARS-CoV-2 (PubMed : 33440148, PubMed : 33514628). Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome, such as vaccinia virus. Plays an important role in amplifying innate immune signaling through recognition of RNA metabolites that are produced during virus infection by ribonuclease L (RNase L). May play an important role in enhancing natural killer cell function and may be involved in growth inhibition and apoptosis in several tumor cell lines.
See full target information IFIH1
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Nature cardiovascular research 3:28-45 PubMed39195894

2024

ATF3 coordinates the survival and proliferation of cardiac macrophages and protects against ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yihui Shao,Yang Li,Yan Liu,Shuolin Zhu,Jianing Wu,Ke Ma,Guoqi Li,Shan Huang,Haichu Wen,Congcong Zhang,Xin-Liang Ma,Ping Li,Jie Du,Yulin Li

Nature communications 14:1456 PubMed36928642

2023

Cryptosporidium uses CSpV1 to activate host type I interferon and attenuate antiparasitic defenses.

Applications

Unspecified application

Species

Unspecified reactive species

Silu Deng,Wei He,Ai-Yu Gong,Min Li,Yang Wang,Zijie Xia,Xin-Tiang Zhang,Andrew S Huang Pacheco,Ankur Naqib,Mark Jenkins,Patrick C Swanson,Kristen M Drescher,Juliane K Strauss-Soukup,Michael Belshan,Xian-Ming Chen

Nature cell biology 25:258-272 PubMed36635503

2023

Targeting Menin disrupts the KMT2A/B and polycomb balance to paradoxically activate bivalent genes.

Applications

Unspecified application

Species

Unspecified reactive species

Christina E Sparbier,Andrea Gillespie,Juliana Gomez,Nishi Kumari,Ali Motazedian,Kah Lok Chan,Charles C Bell,Omer Gilan,Yih-Chih Chan,Sarah Popp,Daniel J Gough,Melanie A Eckersley-Maslin,Sarah-Jane Dawson,Paul J Lehner,Kate D Sutherland,Patricia Ernst,Gerard M McGeehan,Enid Y N Lam,Marian L Burr,Mark A Dawson

Experimental dermatology 31:1165-1176 PubMed35332586

2022

Ultraviolet light induces HERV expression to activate RIG-I signalling pathway in keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoli Min,Meiling Zheng,Yaqin Yu,Jiali Wu,Qiqi Kuang,Zhi Hu,Lianlian Ouyang,Shuang Lu,Ming Zhao

Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology 128:414-422.e2 PubMed35031416

2022

Lower viral loads in subjects with rhinovirus-challenged allergy despite reduced innate immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Feng,Monica G Lawrence,Spencer C Payne,Jose Mattos,Elaine Etter,Julie A Negri,Deborah Murphy,Joshua L Kennedy,John W Steinke,Larry Borish

PLoS pathogens 17:e1010142 PubMed34929018

2021

Viral RNA N6-methyladenosine modification modulates both innate and adaptive immune responses of human respiratory syncytial virus.

Applications

Unspecified application

Species

Unspecified reactive species

Miaoge Xue,Yuexiu Zhang,Haitao Wang,Elizabeth L Kairis,Mijia Lu,Sadeem Ahmad,Zayed Attia,Olivia Harder,Zijie Zhang,Jiangbo Wei,Phylip Chen,Youling Gao,Mark E Peeples,Amit Sharma,Prosper Boyaka,Chuan He,Sun Hur,Stefan Niewiesk,Jianrong Li

Journal of virology 95: PubMed33536170

2021

Nonsegmented Negative-Sense RNA Viruses Utilize -Methyladenosine (mA) as a Common Strategy To Evade Host Innate Immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Mijia Lu,Miaoge Xue,Hai-Tao Wang,Elizabeth L Kairis,Sadeem Ahmad,Jiangbo Wei,Zijie Zhang,Qinzhe Liu,Yuexiu Zhang,Youling Gao,Dominique Garcin,Mark E Peeples,Amit Sharma,Sun Hur,Chuan He,Jianrong Li

Annals of clinical and translational neurology 8:677-686 PubMed33576578

2021

Mitochondrial morphology and MAVS-IFN1 signaling pathway in muscles of anti-MDA5 dermatomyositis.

Applications

Unspecified application

Species

Unspecified reactive species

Yanyan Jiang,Yilin Liu,Yawen Zhao,Yiming Zheng,Meng Yu,Jianwen Deng,Hongjun Hao,Wei Zhang,Zhaoxia Wang,Yun Yuan

Molecular therapy. Nucleic acids 16:105-117 PubMed30861413

2019

Knockdown of IGF-1R Triggers Viral RNA Sensor MDA5- and RIG-I-Mediated Mitochondrial Apoptosis in Colonic Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shu-Qing Wang,Xiang-Yu Yang,Xin-Feng Yu,Shu-Xiang Cui,Xian-Jun Qu

PLoS pathogens 13:e1006713 PubMed29084253

2017

Pivotal role for the ESCRT-II complex subunit EAP30/SNF8 in IRF3-dependent innate antiviral defense.

Applications

WB

Species

Unspecified reactive species

Kattareeya Kumthip,Darong Yang,Nan L Li,Yunzhi Zhang,Meiyun Fan,Aarti Sethuraman,Kui Li
View all publications
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Product promise

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