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Rabbit Recombinant Monoclonal MDC antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Recombinant full length protein - Mouse samples.

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Images

Western blot - Anti-MDC antibody [EPR1362] - BSA and Azide free (AB247994), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDC antibody [EPR1362] - BSA and Azide free (AB247994), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDC antibody [EPR1362] - BSA and Azide free (AB247994), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IF
Human
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Not recommended
Expected
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Recombinant full length protein - Mouse
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species

Mouse

Dilution info

-

Notes

For antigen retrieval, heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

For antigen retrieval, heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

For antigen retrieval, heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species

Recombinant full length protein - Mouse

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Human, Mouse, Recombinant full length protein - Mouse

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant full length protein - Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Rat, Human, Mouse, Recombinant full length protein - Mouse

Dilution info

-

Notes

-

Target data

Function

Chemotactic for activated T-lymphocytes. May play an important role in the collaboration of dendritic cells and B-lymphocytes with T-cells in immune responses.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal MDC antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Recombinant full length protein - Mouse samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR1362

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab247994 is the carrier-free version of Anti-MDC antibody [EPR1362] ab124768.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MDC also known as CCL22 or macrophage-derived chemokine is a small protein involved in immune system signaling. The molecular mass of MDC is approximately 8 kDa. It is expressed in various tissues including lymph nodes thymus and lungs where it mainly acts to regulate immune cell movement. Researchers commonly study this target using MDC assays and CCL22 ELISA to better understand its functions and expression patterns.

Biological function summary

MDC interacts with immune cells to mediate responses such as chemotaxis. It binds to the CCR4 receptor which is found on T cells and other immune cells. MDC does not require complex formation to function; it works independently to attract specific cell types. This chemokine provides a way for immune cells to navigate to sites of inflammation or infection.

Pathways

MDC plays a significant role in inflammatory and immune response pathways. It is integrally linked with the chemokine signaling pathway where it works along with other chemokines to guide immune responses. MDC is related to proteins like CCR4 and other chemokines in these pathways facilitating communication between cells during immune responses and maintaining homeostasis.

Associated diseases and disorders

MDC is implicated in conditions such as allergic responses and autoimmune diseases. Its interaction with CCR4 connects it to disorders like asthma and atopic dermatitis where excessive immune cell recruitment leads to inflammation. Studying MDC levels through macrophage assays and chemokine ELISAs can provide insights into its role in these diseases and help develop potential therapeutic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-MDC antibody [EPR1362] - BSA and Azide free (ab247994), expandable thumbnail

    Western blot - Anti-MDC antibody [EPR1362] - BSA and Azide free (ab247994)

    This data was developed using Anti-MDC antibody [EPR1362] ab124768, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-MDC antibody [EPR1362] (Anti-MDC antibody [EPR1362] ab124768) at 1/1000 dilution

    All lanes: Recombinant full-length MDC protein at 0.005 µg

    Secondary

    All lanes: Goat anti-rabbit HRP-conjugated at 1/2000 dilution

    Predicted band size: 11 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDC antibody [EPR1362] - BSA and Azide free (ab247994), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDC antibody [EPR1362] - BSA and Azide free (ab247994)

    This data was developed using Anti-MDC antibody [EPR1362] ab124768, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling MDC with Anti-MDC antibody [EPR1362] ab124768 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051, 1/500). Counter stained with hematoxylin.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDC antibody [EPR1362] - BSA and Azide free (ab247994), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDC antibody [EPR1362] - BSA and Azide free (ab247994)

    This data was developed using Anti-MDC antibody [EPR1362] ab124768, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling MDC with Anti-MDC antibody [EPR1362] ab124768 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051, 1/500). Counter stained with hematoxylin.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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