JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB242457

Anti-MDC antibody [EPR17002-106] - BSA and Azide free (Capture)

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal MDC antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

View Alternative Names

Abcd1, Scya22, Ccl22, C-C motif chemokine 22, Activated B and dendritic cell-derived, CC chemokine ABCD-1, Small-inducible cytokine A22

1 Images
Sandwich ELISA - Anti-MDC antibody [EPR17002-106] - BSA and Azide free (Capture) (AB242457)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-MDC antibody [EPR17002-106] - BSA and Azide free (Capture) (AB242457)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab204525).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17002-106

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
style
left-column

Product details

ab242457 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse MDC. The recommended pair for sandwich ELISA is:
Capture: ab242457, Mouse MDC Capture Antibody (unconjugated)
Detector: ab242708, Mouse MDC Detector Antibody (unconjugated)
The reference range value is 7.81 - 500 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MDC also known as CCL22 or macrophage-derived chemokine is a small protein involved in immune system signaling. The molecular mass of MDC is approximately 8 kDa. It is expressed in various tissues including lymph nodes thymus and lungs where it mainly acts to regulate immune cell movement. Researchers commonly study this target using MDC assays and CCL22 ELISA to better understand its functions and expression patterns.
Biological function summary

MDC interacts with immune cells to mediate responses such as chemotaxis. It binds to the CCR4 receptor which is found on T cells and other immune cells. MDC does not require complex formation to function; it works independently to attract specific cell types. This chemokine provides a way for immune cells to navigate to sites of inflammation or infection.

Pathways

MDC plays a significant role in inflammatory and immune response pathways. It is integrally linked with the chemokine signaling pathway where it works along with other chemokines to guide immune responses. MDC is related to proteins like CCR4 and other chemokines in these pathways facilitating communication between cells during immune responses and maintaining homeostasis.

MDC is implicated in conditions such as allergic responses and autoimmune diseases. Its interaction with CCR4 connects it to disorders like asthma and atopic dermatitis where excessive immune cell recruitment leads to inflammation. Studying MDC levels through macrophage assays and chemokine ELISAs can provide insights into its role in these diseases and help develop potential therapeutic strategies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Chemotactic for activated T-lymphocytes. May play an important role in the collaboration of dendritic cells and B-lymphocytes with T-cells in immune responses.
See full target information Ccl22
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB223866

Human MDC ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human MDC ELISA Kit (AB223866)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB242720

Anti-Eotaxin 2 antibody [EPR17018-243] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Eotaxin 2 antibody [EPR17018-243] - BSA and Azide free (Detector) (AB242720)

  • 0
  • 0
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB242699

Anti-IL-16 antibody [EPR17283-116] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-IL-16 antibody [EPR17283-116] - BSA and Azide free (Detector) (AB242699)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com