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Rabbit Recombinant Monoclonal MDC1 antibody. Suitable for Flow Cyt (Intra), WB, IHC-P, ICC/IF and reacts with Human samples.

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Images

Western blot - Anti-MDC1 antibody [EPR24360-116] (AB271061), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-MDC1 antibody [EPR24360-116] (AB271061), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-MDC1 antibody [EPR24360-116] (AB271061), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-MDC1 antibody [EPR24360-116] (AB271061), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDC1 antibody [EPR24360-116] (AB271061), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow Cyt (Intra)WBIHC-PICC/IF
Human
Not recommended
Tested
Tested
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Histone reader protein required for checkpoint-mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle (PubMed:12475977, PubMed:12499369, PubMed:12551934, PubMed:12607003, PubMed:12607004, PubMed:12607005, PubMed:12611903, PubMed:14695167, PubMed:15201865, PubMed:15377652, PubMed:16049003, PubMed:16377563, PubMed:30898438). Specifically recognizes and binds histone H2AX phosphorylated at 'Ser-139', a marker of DNA damage, serving as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage sites (PubMed:12607005, PubMed:15201865, PubMed:16049003, PubMed:16377563, PubMed:30898438). Also required for downstream events subsequent to the recruitment of these proteins (PubMed:12607005, PubMed:15201865, PubMed:16049003, PubMed:16377563, PubMed:18582474). These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53/p53 and apoptosis (PubMed:12499369, PubMed:12551934, PubMed:12607004). ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1 (PubMed:12499369, PubMed:12551934, PubMed:12607004). Required for chromosomal stability during mitosis by promoting recruitment of TOPBP1 to DNA double strand breaks (DSBs): TOPBP1 forms filamentous assemblies that bridge MDC1 and tether broken chromosomes during mitosis (PubMed:30898438). Required for the repair of DSBs via homologous recombination by promoting recruitment of NBN component of the MRN complex to DSBs (PubMed:18411307, PubMed:18582474, PubMed:18583988, PubMed:18678890).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal MDC1 antibody. Suitable for Flow Cyt (Intra), WB, IHC-P, ICC/IF and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR24360-116

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

MDC1 also known as mediators of DNA damage checkpoint protein 1 or ABF-M 1711 is an important player in the DNA damage response (DDR). It has a mass of approximately 220 kDa. MDC1 is highly expressed in various human tissues particularly where cell turnover is high like in the bone marrow and lymphoid organs. In cellular operations MDC1 serves as a scaffold protein that coordinates recruitment and activation of DDR machinery at sites of double-strand breaks (DSBs) on DNA.

Biological function summary

This protein plays a vital role in maintaining genomic stability by binding to phosphorylated histone H2AX at DSB sites. It is not a solitary player; MDC1 functions as part of a larger protein complex including factors like RNF8 RNF168 and 53BP1. This complex is essential for amplifying the DDR signal and facilitating the repair process through non-homologous end joining (NHEJ) and homologous recombination (HR). MDC1's interaction with other repair proteins helps to extend the signal required for effective DNA repair.

Pathways

MDC1 significantly influences cellular pathways involving DNA damage sensing and repair and cell cycle checkpoints. It ties closely to the ATM kinase pathway which is activated in response to DSBs. MDC1 aids ATM in phosphorylating downstream targets like CHK2 and p53 for cell cycle arrest. It also connects with BRCA1 interacting in HR repair pathways to ensure accurate repair of DSBs. Both ATM and BRCA1 interactions illustrate MDC1's pivotal role in maintaining DNA integrity.

Associated diseases and disorders

MDC1 expression and functionality have profound implications on cancer and immunodeficiencies. Abnormal MDC1 expression or mutations can lead to impaired DNA repair and genomic instability often associated with tumor progression in cancers such as breast cancer. Moreover MDC1 interacts with proteins like p53 which is a well-known tumor suppressor to hinder cancer development by stopping the cell cycle for repair or triggering apoptosis if the damage is beyond repair. Dysregulation in MDC1-related pathways can also compromise immune system effectiveness further underpinning its significance in disease contexts.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-MDC1 antibody [EPR24360-116] (ab271061), expandable thumbnail

    Western blot - Anti-MDC1 antibody [EPR24360-116] (ab271061)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Fresh lysates were used in this WB.

    This blot was developed using a higher sensitivity ECL substrate.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature ( PMID:12607005; PMID: 26701181 )

    Exposure time: 3 minutes

    All lanes: Western blot - Anti-MDC1 antibody [EPR24360-116] (ab271061) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: Hela transfected with MDC1 siRNA 1 whole cell lysate at 20 µg

    Lane 3: Hela transfected with MDC1 siRNA 2 whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 227 kDa

  • Flow Cytometry (Intracellular) - Anti-MDC1 antibody [EPR24360-116] (ab271061), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MDC1 antibody [EPR24360-116] (ab271061)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling MDC1 with ab271061 at 1/500 dilution (0.1μg)/ red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry (Intracellular) - Anti-MDC1 antibody [EPR24360-116] (ab271061), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-MDC1 antibody [EPR24360-116] (ab271061)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293 (Human embryonic kidney epithelial cell) cells labelling MDC1 with ab271061 at 1/500 dilution (0.1μg)/ red (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-MDC1 antibody [EPR24360-116] (ab271061), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-MDC1 antibody [EPR24360-116] (ab271061)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling MDC1 with ab271061 at 1/50 (10.46 μg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2μg/ml) dilution (Green). Confocal image showing nuclear staining in HeLa cells is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5μg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2μg/ml) dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDC1 antibody [EPR24360-116] (ab271061), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDC1 antibody [EPR24360-116] (ab271061)

    Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labelling MDC1 with ab271061 at 1/500 (1.046 μg/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Nuclear staining on human ovarian cancer. The section was incubated with ab271061 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDC1 antibody [EPR24360-116] (ab271061), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDC1 antibody [EPR24360-116] (ab271061)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling MDC1 with ab271061 at 1/500 (1.046 μg/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Nuclear staining on human testis. The section was incubated with ab271061 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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