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AB241048

Anti-MDC1 antibody [P2B11]

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(7 Publications)

Mouse Monoclonal MDC1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Mdc1.

View Alternative Names

KIAA0170, NFBD1, MDC1, Mediator of DNA damage checkpoint protein 1, Nuclear factor with BRCT domains 1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-MDC1 antibody [P2B11] (AB241048)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MDC1 antibody [P2B11] (AB241048)

Immunocytochemistry/Immunofluorescence analysis of NIH/3T3 (Mouse embryo fibroblast cell line) cells labeling MDC1 (Green) using ab241048 at 1 : 100 for 60 minutes at RT. Cells were fixed in 4% formaldehyde for 15 minutes at RT.

Secondary antibody : Goat anti-Mouse ATTO 488 at 1 : 100 for 60 minutes at RT.

Counterstain : DAPI (blue) nuclear stain at 1 : 5000, 5 minutes RT.

(A) DAPI (blue) nuclear stain (B) ab241048 (C) Composite.

Western blot - Anti-MDC1 antibody [P2B11] (AB241048)
  • WB

Supplier Data

Western blot - Anti-MDC1 antibody [P2B11] (AB241048)

Block : 5% Skimmed milk in 1X TBST

All lanes:

Western blot - Anti-MDC1 antibody [P2B11] (ab241048) at 1/1000 dilution

Lane 1:

Mouse cortex lysate at 10 µg

Lane 2:

Mouse cerebellum lysate at 10 µg

Secondary

All lanes:

Goat anti-Mouse HRP-IgG for 60 minutes at RT at 1/2000 dilution

Predicted band size: 227 kDa

true

Exposure time: 5min

Western blot - Anti-MDC1 antibody [P2B11] (AB241048)
  • WB

Supplier Data

Western blot - Anti-MDC1 antibody [P2B11] (AB241048)

Block : 5% Skimmed milk in 1X TBST.

All lanes:

Western blot - Anti-MDC1 antibody [P2B11] (ab241048) at 1/1000 dilution

All lanes:

Human 293Trap cell lysates at 30 µg

Secondary

All lanes:

Goat anti-Mouse HRP-IgG for 60 minutes at RT at 1/2000 dilution

Predicted band size: 227 kDa

true

Exposure time: 5min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

P2B11

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Mouse Mdc1. The exact immunogen used to generate this antibody is proprietary information.

Q5PSV9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from TCS.
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MDC1 also known as mediators of DNA damage checkpoint protein 1 or ABF-M 1711 is an important player in the DNA damage response (DDR). It has a mass of approximately 220 kDa. MDC1 is highly expressed in various human tissues particularly where cell turnover is high like in the bone marrow and lymphoid organs. In cellular operations MDC1 serves as a scaffold protein that coordinates recruitment and activation of DDR machinery at sites of double-strand breaks (DSBs) on DNA.
Biological function summary

This protein plays a vital role in maintaining genomic stability by binding to phosphorylated histone H2AX at DSB sites. It is not a solitary player; MDC1 functions as part of a larger protein complex including factors like RNF8 RNF168 and 53BP1. This complex is essential for amplifying the DDR signal and facilitating the repair process through non-homologous end joining (NHEJ) and homologous recombination (HR). MDC1's interaction with other repair proteins helps to extend the signal required for effective DNA repair.

Pathways

MDC1 significantly influences cellular pathways involving DNA damage sensing and repair and cell cycle checkpoints. It ties closely to the ATM kinase pathway which is activated in response to DSBs. MDC1 aids ATM in phosphorylating downstream targets like CHK2 and p53 for cell cycle arrest. It also connects with BRCA1 interacting in HR repair pathways to ensure accurate repair of DSBs. Both ATM and BRCA1 interactions illustrate MDC1's pivotal role in maintaining DNA integrity.

MDC1 expression and functionality have profound implications on cancer and immunodeficiencies. Abnormal MDC1 expression or mutations can lead to impaired DNA repair and genomic instability often associated with tumor progression in cancers such as breast cancer. Moreover MDC1 interacts with proteins like p53 which is a well-known tumor suppressor to hinder cancer development by stopping the cell cycle for repair or triggering apoptosis if the damage is beyond repair. Dysregulation in MDC1-related pathways can also compromise immune system effectiveness further underpinning its significance in disease contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Histone reader protein required for checkpoint-mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle (PubMed : 12475977, PubMed : 12499369, PubMed : 12551934, PubMed : 12607003, PubMed : 12607004, PubMed : 12607005, PubMed : 12611903, PubMed : 14695167, PubMed : 15201865, PubMed : 15377652, PubMed : 16049003, PubMed : 16377563, PubMed : 30898438). Specifically recognizes and binds histone H2AX phosphorylated at 'Ser-139', a marker of DNA damage, serving as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage sites (PubMed : 12607005, PubMed : 15201865, PubMed : 16049003, PubMed : 16377563, PubMed : 30898438). Also required for downstream events subsequent to the recruitment of these proteins (PubMed : 12607005, PubMed : 15201865, PubMed : 16049003, PubMed : 16377563, PubMed : 18582474). These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53/p53 and apoptosis (PubMed : 12499369, PubMed : 12551934, PubMed : 12607004). ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1 (PubMed : 12499369, PubMed : 12551934, PubMed : 12607004). Required for chromosomal stability during mitosis by promoting recruitment of TOPBP1 to DNA double strand breaks (DSBs) : TOPBP1 forms filamentous assemblies that bridge MDC1 and tether broken chromosomes during mitosis (PubMed : 30898438). Required for the repair of DSBs via homologous recombination by promoting recruitment of NBN component of the MRN complex to DSBs (PubMed : 18411307, PubMed : 18582474, PubMed : 18583988, PubMed : 18678890).
See full target information MDC1
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 53: PubMed39657788

2024

Novel BRCA1-PLK1-CIP2A axis orchestrates homologous recombination-mediated DNA repair to maintain chromosome integrity during oocyte meiosis.

Applications

Unspecified application

Species

Unspecified reactive species

Crystal Lee,Jeong Su Oh

Nucleic acids research 51:4899-4913 PubMed36999590

2023

Oocytes can repair DNA damage during meiosis via a microtubule-dependent recruitment of CIP2A-MDC1-TOPBP1 complex from spindle pole to chromosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Jiyeon Leem,Jae-Sung Kim,Jeong Su Oh

Cell proliferation 56:e13384 PubMed36564861

2022

Selective utilization of non-homologous end-joining and homologous recombination for DNA repair during meiotic maturation in mouse oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Crystal Lee,Jiyeon Leem,Jeong Su Oh

Histology and histopathology 37:909-917 PubMed35467005

2022

MiR-486-5p specifically suppresses SAPCD2 expression, which attenuates the aggressive phenotypes of lung adenocarcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Desheng Wei

Cancers 14: PubMed35454780

2022

SFN Enhanced the Radiosensitivity of Cervical Cancer Cells via Activating LATS2 and Blocking Rad51/MDC1 Recruitment to DNA Damage Site.

Applications

Unspecified application

Species

Unspecified reactive species

Shiyu Wang,Yanan Wang,Xiangnan Liu,Yongbin Yang,Sufang Wu,Yuan Liu

Cellular and molecular life sciences : CMLS 79:200 PubMed35320416

2022

MDC1 is essential for G2/M transition and spindle assembly in mouse oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Jiyeon Leem,Jeong Su Oh

Cell death & disease 12:1138 PubMed34880209

2021

FDI-6 inhibits the expression and function of FOXM1 to sensitize BRCA-proficient triple-negative breast cancer cells to Olaparib by regulating cell cycle progression and DNA damage repair.

Applications

Unspecified application

Species

Unspecified reactive species

Shu-Ping Wang,Shi-Qi Wu,Shi-Hui Huang,Yi-Xuan Tang,Liu-Qiong Meng,Feng Liu,Qi-Hua Zhu,Yun-Gen Xu
View all publications
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