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AB251808

Anti-MDFIC antibody

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Rabbit Polyclonal MDFIC antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MDFIC aa 50 to C-terminus.

View Alternative Names

MyoD family inhibitor domain-containing protein, I-mfa domain-containing protein, hIC, MDFIC

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-MDFIC antibody (AB251808)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MDFIC antibody (AB251808)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for MDFIC (green) using ab251808 at 4 µg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDFIC antibody (AB251808)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDFIC antibody (AB251808)

Paraffin-embedded human appendix tissue stained for MDFIC using ab251808 at 1/50 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human MDFIC aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9P1T7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MDFIC also known as MyoD family inhibitor domain containing is a protein that inhibits MyoD family of transcription factors. The mass of MDFIC is approximately 28 kDa. It plays a role in regulating gene transcription by binding to DNA and interfering with MyoD family members' ability to activate target genes. Researchers have detected MDFIC expression in various tissues with higher levels in the heart skeletal muscle and brain. MDFIC influences cellular differentiation processes in these tissues.
Biological function summary

MDFIC serves as a negative regulator in muscle cell differentiation by forming a complex with other transcription factors. This complex modulates the transcriptional activity of genes that manage muscle development and regeneration. MDFIC is involved in maintaining the balance between cell proliferation and differentiation ensuring that cells do not differentiate prematurely. This function allows tissues to grow and repair effectively.

Pathways

MDFIC participates in the regulation of myogenesis and signal transduction pathways. It interacts with the MyoD family especially MyoD and myogenin to modulate muscle-specific gene expression. Myogenesis the process of muscle cell formation requires a delicate regulation where MDFIC affects the balance by inhibiting transcription factors' binding affinity. Additionally MDFIC bears functional relevance in pathways involving nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) where it shifts cellular responses to external stimuli like stress or inflammation.

MDFIC involvement extends to certain muscular dystrophies where dysregulation can lead to impaired muscle repair and degeneration. MDFIC's connection with MyoD family proteins is significant in these disorders contributing to the pathology of muscle weakness and wasting. Furthermore MDFIC has relevance in cardiac pathologies such as cardiac hypertrophy where its regulation mechanism impacts heart muscle cells' ability to cope with stress and workload. The interplay between MDFIC and NF-kB signaling in cardiac tissues affects inflammatory responses which plays a part in disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required to control the activity of various transcription factors through their sequestration in the cytoplasm. Retains nuclear Zic proteins ZIC1, ZIC2 and ZIC3 in the cytoplasm and inhibits their transcriptional activation (By similarity). Modulates the expression from cellular promoters. Binds to the axin complex, resulting in an increase in the level of free beta-catenin (PubMed : 12192039). Affects axin regulation of the WNT and JNK signaling pathways (PubMed : 12192039). Involved in the development of lymphatic vessel valves (By similarity). Required to promote lymphatic endothelial cell migration, in a process that involves down-regulation of integrin beta 1 activation and control of cell adhesion to the extracellular matrix (PubMed : 35235341). Regulates the activity of mechanosensitive Piezo channel (PubMed : 37590348).. (Microbial infection) Modulates the expression from viral promoters. Down-regulates Tat-dependent transcription of the human immunodeficiency virus type 1 (HIV-1) LTR by interacting with HIV-1 Tat and Rev and impairing their nuclear import, probably by rendering the NLS domains inaccessible to importin-beta (PubMed : 12944466, PubMed : 16260749, Ref.6). Also stimulates activation of human T-cell leukemia virus type I (HTLV-I) LTR (PubMed : 10671520).
See full target information MDFIC
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Product promise

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