AB175455

Anti-MDH1 antibody

Name: Anti-MDH1 antibody (ab175455) | Abcam
Brand: Abcam Limited
SKU: AB175455

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(4 Publications)

Goat Polyclonal MDH1 antibody. Suitable for WB and reacts with Mouse, Rat, Pig, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human MDH1 aa 100-150.

View Alternative Names

MDHA, MDH1, Aromatic alpha-keto acid reductase, Cytosolic malate dehydrogenase, KAR

2 Images

Supplier Data

Western blot - Anti-MDH1 antibody (AB175455)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-MDH1 antibody (ab175455) at 0.01 µg/mL

Lane 1:

Human heart lysate (in RIPA buffer) at 35 µg

Lane 2:

Mouse heart lysate (in RIPA buffer) at 35 µg

Lane 3:

Rat heart lysate (in RIPA buffer) at 35 µg

Predicted band size: 36 kDa

Observed band size: 36 kDa

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Supplier Data

Western blot - Anti-MDH1 antibody (AB175455)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-MDH1 antibody (ab175455) at 0.01 µg/mL

All lanes:

Pig heart lysate ( in RIPA buffer) at 35 µg

Predicted band size: 36 kDa

Observed band size: 36 kDa

true

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human, Pig

Applications

applications

Immunogen

Synthetic Peptide within Human MDH1 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

P40925

Specificity

ab175455 is expected to recognize all reported isoforms (NP_001186040.1; NP_005908.1; NP_001186041.1).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01-0.03 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01-0.03 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01-0.03 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01-0.03 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product." }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab175455 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The cytosolic malate dehydrogenase commonly called MDH1 or malate dehydrogenase 1 is an enzyme that plays an important role in the citric acid cycle. Its main function is to catalyze the reversible conversion of malate to oxaloacetate using NAD+ as a cofactor. This enzyme has an approximate molecular mass of 36 kDa. MDH1 is expressed in the cytosol of various cell types throughout the body. It is critical in enabling cells to efficiently convert biochemical energy from nutrients into ATP which is vital for cellular function and survival.

Biological function summary

This enzyme supports numerous cellular processes by maintaining redox balance and facilitating energy production. MDH1 acts independently and isn't part of any larger protein complex. However its efficiency directly impacts the cycling of metabolites necessary for cellular respiration and energy metabolism. By converting malate into oxaloacetate MDH1 directly influences processes critical for the maintenance of cell health and function.

Pathways

MDH1 is an integral component of the tricarboxylic acid (TCA) cycle and the malate-aspartate shuttle. In the TCA cycle it plays an important role in the conversion steps that drive energy generation within cells. Additionally the malate-aspartate shuttle is an important pathway connecting cytosolic and mitochondrial processes by facilitating the exchange of reducing equivalents. Through these pathways MDH1 interacts and collaborates with proteins like aspartate aminotransferase (GOT1) and mitochondrial malate dehydrogenase (MDH2) showcasing the interconnected nature of metabolic pathways.

Dysfunction or abnormal expression of MDH1 has been associated with various conditions including cancer and metabolic syndromes. In cancer aberrant MDH1 activity can lead to altered energy metabolism promoting tumor progression and survival. Additionally MDH1's role in glucose and lipid metabolism makes it relevant in metabolic syndrome where improper energy balance can exacerbate disease states. Proteins connected through these associations include hypoxia-inducible factor 1-alpha (HIF-1α) in cancer metabolism and insulin signaling proteins in metabolic syndromes further illustrating the broader implications of MDH1's function in health and disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH (PubMed : 2449162, PubMed : 3052244). Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation (PubMed : 31538237). Catalyzes the reduction of 2-oxoglutarate to 2-hydroxyglutarate, leading to elevated reactive oxygen species (ROS) (PubMed : 34012073).

See full target information MDH1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature metabolism 6:2138-2156 PubMed39472756

2024

Branched-chain α-ketoacids aerobically activate HIF1α signalling in vascular cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wusheng Xiao,Nishith Shrimali,Niv Vigder,William M Oldham,Clary B Clish,Huamei He,Samantha J Wong,Bradley M Wertheim,Elena Arons,Marcia C Haigis,Jane A Leopold,Joseph Loscalzo

Heliyon 9:e18134 PubMed37539197

2023

Proteomics reveals defective peroxisomal fatty acid oxidation during the progression of acute kidney injury and repair.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Chen,Quan-You Zheng,Li-Ming Wang,Jia Luo,Ke-Hong Chen,Ya-Ni He

Computational and mathematical methods in medicine 2022:2023500 PubMed36158123

2022

MDH1 and MDH2 Promote Cell Viability of Primary AT2 Cells by Increasing Glucose Uptake.

Applications

Unspecified application

Species

Unspecified reactive species

Mu Hu,JieLai Yang,Yang Xu,Jiao Liu

Molecular medicine reports 20:3793-3801 PubMed31485626

2019

Overexpression of PTEN may increase the effect of pemetrexed on A549 cells via inhibition of the PI3K/AKT/mTOR pathway and carbohydrate metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Li,Junkai Zhang,Ya Su,Yiling Hou,Zhenguo Wang,Lin Zhao,Shengkai Sun,Hao Fu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MDH1 antibody

Western blot - Anti-MDH1 antibody (AB175455)

Western blot - Anti-MDH1 antibody (AB175455)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise