Anti-MDH1 antibody [EPR13596(B)]

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MDH1 antibody [EPR13596(B)] (AB181091)

Intracellular flow cytometric analysis of 2%paraformaldehyde-fixedJurkat cells labeling MDH1 with ab181091 at 1/100 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

• WB

Supplier Data

Western blot - Anti-MDH1 antibody [EPR13596(B)] (AB181091)

All lanes:

Western blot - Anti-MDH1 antibody [EPR13596(B)] (ab181091) at 1/2000 dilution

Lane 1:

Human spleen lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

• WB

Supplier Data

Western blot - Anti-MDH1 antibody [EPR13596(B)] (AB181091)

ab181091 was shown to react with MDH1 in wild-type HAP1 cells in Western blot with loss of signal observed in a MDH1 knockout cell line. Wild-type HAP1 and MDH1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab181091 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-MDH1 antibody [EPR13596(B)] (ab181091) at 1/1000 dilution

Lane 1:

Wild-type HAP1 lysate at 20 µg

Lane 2:

MDH1 knock-out HAP1 lysate at 20 µg

false