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AB250339

Anti-MDH1 antibody [EPR13596(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal MDH1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

MDHA, MDH1, Aromatic alpha-keto acid reductase, Cytosolic malate dehydrogenase, KAR

2 Images
Flow Cytometry (Intracellular) - Anti-MDH1 antibody [EPR13596(B)] - BSA and Azide free (AB250339)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MDH1 antibody [EPR13596(B)] - BSA and Azide free (AB250339)

This data was developed using ab181091, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling MDH1 with ab181091 at 1/100 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Western blot - Anti-MDH1 antibody [EPR13596(B)] - BSA and Azide free (AB250339)
  • WB

Supplier Data

Western blot - Anti-MDH1 antibody [EPR13596(B)] - BSA and Azide free (AB250339)

This data was developed using ab181091, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MDH1 antibody [EPR13596(B)] (<a href='/en-us/products/primary-antibodies/mdh1-antibody-epr13596b-ab181091'>ab181091</a>) at 1/2000 dilution

Lane 1:

Human spleen lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13596(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250339 is the carrier-free version of ab181091.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The cytosolic malate dehydrogenase commonly called MDH1 or malate dehydrogenase 1 is an enzyme that plays an important role in the citric acid cycle. Its main function is to catalyze the reversible conversion of malate to oxaloacetate using NAD+ as a cofactor. This enzyme has an approximate molecular mass of 36 kDa. MDH1 is expressed in the cytosol of various cell types throughout the body. It is critical in enabling cells to efficiently convert biochemical energy from nutrients into ATP which is vital for cellular function and survival.
Biological function summary

This enzyme supports numerous cellular processes by maintaining redox balance and facilitating energy production. MDH1 acts independently and isn't part of any larger protein complex. However its efficiency directly impacts the cycling of metabolites necessary for cellular respiration and energy metabolism. By converting malate into oxaloacetate MDH1 directly influences processes critical for the maintenance of cell health and function.

Pathways

MDH1 is an integral component of the tricarboxylic acid (TCA) cycle and the malate-aspartate shuttle. In the TCA cycle it plays an important role in the conversion steps that drive energy generation within cells. Additionally the malate-aspartate shuttle is an important pathway connecting cytosolic and mitochondrial processes by facilitating the exchange of reducing equivalents. Through these pathways MDH1 interacts and collaborates with proteins like aspartate aminotransferase (GOT1) and mitochondrial malate dehydrogenase (MDH2) showcasing the interconnected nature of metabolic pathways.

Dysfunction or abnormal expression of MDH1 has been associated with various conditions including cancer and metabolic syndromes. In cancer aberrant MDH1 activity can lead to altered energy metabolism promoting tumor progression and survival. Additionally MDH1's role in glucose and lipid metabolism makes it relevant in metabolic syndrome where improper energy balance can exacerbate disease states. Proteins connected through these associations include hypoxia-inducible factor 1-alpha (HIF-1α) in cancer metabolism and insulin signaling proteins in metabolic syndromes further illustrating the broader implications of MDH1's function in health and disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH (PubMed : 2449162, PubMed : 3052244). Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation (PubMed : 31538237). Catalyzes the reduction of 2-oxoglutarate to 2-hydroxyglutarate, leading to elevated reactive oxygen species (ROS) (PubMed : 34012073).
See full target information MDH1
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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