AB232190

Anti-MDH1 antibody [EPR13597(B)] - BSA and Azide free

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal MDH1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

MDHA, MDH1, Aromatic alpha-keto acid reductase, Cytosolic malate dehydrogenase, KAR

2 Images

Flow Cytometry (Intracellular) - Anti-MDH1 antibody [EPR13597(B)] - BSA and Azide free (AB232190)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MDH1 antibody [EPR13597(B)] - BSA and Azide free (AB232190)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling MDH1 with ab180152 at 1/20 dilution followed by Goat anti rabbit IgG (FITC) at 1/75 dilution (red), compared to a Rabbit IgG control (green).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180152).

Unknown

Immunoprecipitation - Anti-MDH1 antibody [EPR13597(B)] - BSA and Azide free (AB232190)

Western blot analysis of HepG2 cell lysate immunoprecipitated with ab180152 at 1/50 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180152).

All lanes:

Immunoprecipitation - Anti-MDH1 antibody [EPR13597(B)] - C-terminal (<a href='/en-us/products/primary-antibodies/mdh1-antibody-epr13597b-c-terminal-ab180152'>ab180152</a>)

Predicted band size: 36 kDa

false

Unconjugated

Anti-MDH1 antibody [EPR13597(B)] - C-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13597(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

Product details

ab232190 is the carrier-free version of ab180152.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The cytosolic malate dehydrogenase commonly called MDH1 or malate dehydrogenase 1 is an enzyme that plays an important role in the citric acid cycle. Its main function is to catalyze the reversible conversion of malate to oxaloacetate using NAD+ as a cofactor. This enzyme has an approximate molecular mass of 36 kDa. MDH1 is expressed in the cytosol of various cell types throughout the body. It is critical in enabling cells to efficiently convert biochemical energy from nutrients into ATP which is vital for cellular function and survival.

Biological function summary

This enzyme supports numerous cellular processes by maintaining redox balance and facilitating energy production. MDH1 acts independently and isn't part of any larger protein complex. However its efficiency directly impacts the cycling of metabolites necessary for cellular respiration and energy metabolism. By converting malate into oxaloacetate MDH1 directly influences processes critical for the maintenance of cell health and function.

Pathways

MDH1 is an integral component of the tricarboxylic acid (TCA) cycle and the malate-aspartate shuttle. In the TCA cycle it plays an important role in the conversion steps that drive energy generation within cells. Additionally the malate-aspartate shuttle is an important pathway connecting cytosolic and mitochondrial processes by facilitating the exchange of reducing equivalents. Through these pathways MDH1 interacts and collaborates with proteins like aspartate aminotransferase (GOT1) and mitochondrial malate dehydrogenase (MDH2) showcasing the interconnected nature of metabolic pathways.

Dysfunction or abnormal expression of MDH1 has been associated with various conditions including cancer and metabolic syndromes. In cancer aberrant MDH1 activity can lead to altered energy metabolism promoting tumor progression and survival. Additionally MDH1's role in glucose and lipid metabolism makes it relevant in metabolic syndrome where improper energy balance can exacerbate disease states. Proteins connected through these associations include hypoxia-inducible factor 1-alpha (HIF-1α) in cancer metabolism and insulin signaling proteins in metabolic syndromes further illustrating the broader implications of MDH1's function in health and disease.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH (PubMed : 2449162, PubMed : 3052244). Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation (PubMed : 31538237). Catalyzes the reduction of 2-oxoglutarate to 2-hydroxyglutarate, leading to elevated reactive oxygen species (ROS) (PubMed : 34012073).

See full target information MDH1

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Translational lung cancer research 10:2523-2538 PubMed34295659

2021

One carbon metabolism in human lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sha Yao,Luogen Peng,Omar Elakad,Stefan Küffer,Marc Hinterthaner,Bernhard C Danner,Alexander von Hammerstein-Equord,Philipp Ströbel,Hanibal Bohnenberger

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MDH1 antibody [EPR13597(B)] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-MDH1 antibody [EPR13597(B)] - BSA and Azide free (AB232190)

Immunoprecipitation - Anti-MDH1 antibody [EPR13597(B)] - BSA and Azide free (AB232190)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Alternative Products

Primary Antibodies

Primary Antibodies

Assay Kits

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise