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AB110317

Anti-MDH2 antibody [2F5AF8]

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(4 Publications)

Mouse Monoclonal MDH2 antibody. Suitable for IP, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 4 publications.

View Alternative Names

MDH2

2 Images
Immunoprecipitation - Anti-MDH2 antibody [2F5AF8] (AB110317)
  • IP

Unknown

Immunoprecipitation - Anti-MDH2 antibody [2F5AF8] (AB110317)

ab110317 pulls down the 33kDa MDH2 protein from Human heart homogenate (1), Rat liver homogenate (2), Mouse liver homogenate (3) and HepG2 whole cell lysate (4). The identity of this protein was confirmed by mass spectrometry. This gel was stained with coomassie.

All lanes:

Immunoprecipitation - Anti-MDH2 antibody [2F5AF8] (ab110317)

Predicted band size: 36 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-MDH2 antibody [2F5AF8] (AB110317)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MDH2 antibody [2F5AF8] (AB110317)

Immunocytochemistry image of stained fibroblast cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with the ab110317 (1 μg/mL) for 2 hours at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to the mitochondrial matrix

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2F5AF8

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

Form
Liquid
Purification technique
Proprietary technique
Purification notes
The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity >95% by SDS-PAGE.
Storage buffer
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MDH2 also known as malate dehydrogenase 2 or mitochondrial malate dehydrogenase is an enzyme with a molecular mass of approximately 35 kDa. This enzyme catalyzes the conversion of malate to oxaloacetate using NAD+ as a cofactor. It is predominantly expressed in the mitochondria where it plays an important role in cellular respiration. The enzyme enables the malate dehydrogenase reaction which is key for the functioning of the tricarboxylic acid cycle.
Biological function summary

MDH2 participates in the critical process of energy production within the cell. While it does not form a complex itself its activity is intimately connected with other enzymes in mitochondrial energy metabolism. The malate dehydrogenase assay often measures the activity of MDH2 to understand the metabolic status of cells. By facilitating the oxidation of malate MDH2 aids in maintaining the efficiency of the mitochondrial electron transport chain by regenerating NADH.

Pathways

The enzyme is essential in the tricarboxylic acid (TCA) cycle and the malate-aspartate shuttle. In the TCA cycle MDH2 collaborates with enzymes like citrate synthase and isocitrate dehydrogenase to assist in the conversion of acetyl-CoA into energy-rich molecules. The malate-aspartate shuttle on the other hand involves MDH2 working closely with aspartate transaminase to transfer reducing equivalents into the mitochondria. These pathways highlight MDH2's importance in cellular energy homeostasis.

Mutations or dysregulation in MDH2 have connections to certain metabolic conditions and cancers. For example alterations in MDH2 activity might contribute to conditions like mitochondrial myopathy altering energy metabolism. Moreover MDH2 is associated with NADH-producing enzymes whose dysregulation can support oncogenic pathways in cancer. Understanding these associations helps researchers pursue therapeutic targets that modulate MDH2 activity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Malate Dehydrogenase
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The British journal of nutrition 130:1473-1486 PubMed36815302

2023

Intermittent fasting promotes adipocyte mitochondrial fusion through Sirt3-mediated deacetylation of Mdh2.

Applications

Unspecified application

Species

Unspecified reactive species

Yizhou Li,Juntong Liang,Xin Tian,Qi Chen,Longbo Zhu,Han Wang,Zunhai Liu,Xulei Dai,Chenqi Bian,Chao Sun

Nature communications 13:7414 PubMed36460681

2022

Nuclear localization of mitochondrial TCA cycle enzymes modulates pluripotency via histone acetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Qi Long,Hao Wu,Yanshuang Zhou,Lifan Duan,Hao Yuan,Yingzhe Ding,Yile Huang,Yi Wu,Jinyu Huang,Delong Liu,Baodan Chen,Jian Zhang,Juntao Qi,Shiwei Du,Linpeng Li,Yang Liu,Zifeng Ruan,Zihuang Liu,Zichao Liu,Yifan Zhao,Jianghuan Lu,Junwei Wang,Wai-Yee Chan,Xingguo Liu

Clinical and translational medicine 10:e172 PubMed32997407

2020

SIRT5 deficiency enhances the proliferative and therapeutic capacities of adipose-derived mesenchymal stem cells via metabolic switching.

Applications

Unspecified application

Species

Unspecified reactive species

Tiantong Ou,Wenlong Yang,Wenjia Li,Yijing Lu,Zheng Dong,Hongming Zhu,Xiaolei Sun,Zhen Dong,Xinyu Weng,Suchi Chang,Hua Li,Yufan Li,Zhiwei Qiu,Kai Hu,Aijun Sun,Junbo Ge

PloS one 10:e0117294 PubMed25635834

2015

Dietary L-arginine supplementation affects the skeletal longissimus muscle proteome in finishing pigs.

Applications

WB

Species

Pig

Xianyong Ma,Chuntian Zheng,Youjun Hu,Li Wang,Xuefen Yang,Zongyong Jiang
View all publications
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