Rabbit Polyclonal MDH2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MDH2 aa 1-300.
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- Aging and disease 13:1532-15452022Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinghao Yuan,Yangyang Wang,Jie Wen,Feng Jing,Qian Zou,Yinshuang Pu,Tingyu Pan,Zhiyou CaiPubMed 36186135
- Cell reports 40:1111052022Skeletal progenitors preserve proliferation and self-renewal upon inhibition of mitochondrial respiration by rerouting the TCA cycle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuillaume Tournaire,Shauni Loopmans,Steve Stegen,Gianmarco Rinaldi,Guy Eelen,Sophie Torrekens,Karen Moermans,Peter Carmeliet,Bart Ghesquière,Bernard Thienpont,Sarah-Maria Fendt,Nick van Gastel,Geert CarmelietPubMed 35905715
- Disease models & mechanisms 13:2020Teleological role of L-2-hydroxyglutarate dehydrogenase in the kidney.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGarrett Brinkley et. al.PubMed 32928875
- Molecular genetics and metabolism 128:444-4512019Tricarboxylic acid cycle enzyme activities in a mouse model of methylmalonic aciduria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesParith Wongkittichote et. al.PubMed 31648943
- Clinical cancer research : an official journal of the American Association for Cancer Research 24:6433-64462018Biochemical and Epigenetic Insights into L-2-Hydroxyglutarate, a Potential Therapeutic Target in Renal Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSandeep Shelar,Eun-Hee Shim,Garrett J Brinkley,Anirban Kundu,Francesca Carobbio,Tyler Poston,Jubilee Tan,Vishwas Parekh,Daniel Benson,David K Crossman,Phillip J Buckhaults,Dinesh Rakheja,Richard Kirkman,Yusuke Sato,Seishi Ogawa,Shilpa Dutta,Sadanandan E Velu,Ethan Emberley,Alison Pan,Jason Chen,Tony Huang,Devin Absher,Anja Becker,Conrad Kunick,Sunil SudarshanPubMed 30108105
- Cell 169:1051-1065.e182017The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeniz Simsek,Gerald C Tiu,Ryan A Flynn,Gun W Byeon,Kathrin Leppek,Adele F Xu,Howard Y Chang,Maria BarnaPubMed 28575669
- Nature chemical biology 13:494-5002017L-2-Hydroxyglutarate production arises from noncanonical enzyme function at acidic pH.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrew M Intlekofer et. al.PubMed 28263965
- PloS one 11:e01603842016Novel Metabolic Abnormalities in the Tricarboxylic Acid Cycle in Peripheral Cells From Huntington's Disease Patients.
- Proteomics 14:1480-932014Mechanisms of kidney repair by human mesenchymal stromal cells after ischemia: a comprehensive view using label-free MS(E).Applications:Unspecified applicationReactive species:Unspecified reactive speciesMilene R da Costa,Luciana Pizzatti,Rafael S Lindoso,Julliana Ferreira Sant'Anna,Barbara DuRocher,Eliana Abdelhay,Adalberto VieyraPubMed 24723500
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MDH2 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link P40926
Consider this alternative
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Tested | Expected |
| Rat | Tested | Expected | Expected |
| Zebrafish | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Rat | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes EDTA based, pH 8.0 buffer, 15min |
Species Human | Dilution info 1/100 - 1/1000 | Notes EDTA based, pH 8.0 buffer, 15min |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Alternative names
MDH2
Recommended products
Rabbit Polyclonal MDH2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MDH2 aa 1-300.
Alternative names
MDH2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MDH2 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link P40926
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MDH2 also known as malate dehydrogenase 2 or mitochondrial malate dehydrogenase is an enzyme with a molecular mass of approximately 35 kDa. This enzyme catalyzes the conversion of malate to oxaloacetate using NAD+ as a cofactor. It is predominantly expressed in the mitochondria where it plays an important role in cellular respiration. The enzyme enables the malate dehydrogenase reaction which is key for the functioning of the tricarboxylic acid cycle.
- Biological function summary
MDH2 participates in the critical process of energy production within the cell. While it does not form a complex itself its activity is intimately connected with other enzymes in mitochondrial energy metabolism. The malate dehydrogenase assay often measures the activity of MDH2 to understand the metabolic status of cells. By facilitating the oxidation of malate MDH2 aids in maintaining the efficiency of the mitochondrial electron transport chain by regenerating NADH.
- Pathways
The enzyme is essential in the tricarboxylic acid (TCA) cycle and the malate-aspartate shuttle. In the TCA cycle MDH2 collaborates with enzymes like citrate synthase and isocitrate dehydrogenase to assist in the conversion of acetyl-CoA into energy-rich molecules. The malate-aspartate shuttle on the other hand involves MDH2 working closely with aspartate transaminase to transfer reducing equivalents into the mitochondria. These pathways highlight MDH2’s importance in cellular energy homeostasis.
- Associated diseases and disorders
Mutations or dysregulation in MDH2 have connections to certain metabolic conditions and cancers. For example alterations in MDH2 activity might contribute to conditions like mitochondrial myopathy altering energy metabolism. Moreover MDH2 is associated with NADH-producing enzymes whose dysregulation can support oncogenic pathways in cancer. Understanding these associations helps researchers pursue therapeutic targets that modulate MDH2 activity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
Western blot - Anti-MDH2 antibody (ab96193)
10% SDS-PAGE
All lanes: Western blot - Anti-MDH2 antibody (ab96193) at 1/1000 dilution
Lane 1: Wild-type 293T cell extract at 30 µg
Lane 2: Knockout 293T cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 36 kDa
Immunocytochemistry/ Immunofluorescence - Anti-MDH2 antibody (ab96193)
Immunofluorescence staining of ice-cold methanol-fixed (5 minutes) HeLa cells stained for MDH2 with ab96193 at 1:500 dilution. Blue: Hoechst 33342 staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDH2 antibody (ab96193)
Immunohistochemical analysis of paraffin embedded mouse heart tissue labelling MDH2 with ab96193 antibody at 1/500 dilution at the mitochondria.
Western blot - Anti-MDH2 antibody (ab96193)
All lanes: Western blot - Anti-MDH2 antibody (ab96193) at 1/1000 dilution
Lane 1: 293T whole cell lysate at 30 µg
Lane 2: A431 whole cell lysate at 30 µg
Lane 3: HeLa whole cell lysate at 30 µg
Lane 4: HepG2 whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 36 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDH2 antibody (ab96193)
Immunohistochemical analysis of paraffin embedded H1299 xenograft tissue labelling MDH2 with ab96193 antibody at 1/500 dilution.
Western blot - Anti-MDH2 antibody (ab96193)
10% SDS PAGE
All lanes: Western blot - Anti-MDH2 antibody (ab96193) at 1/10000 dilution
All lanes: Mouse brain at 20 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 36 kDa
Western blot - Anti-MDH2 antibody (ab96193)
10% SDS-PAGE
All lanes: Western blot - Anti-MDH2 antibody (ab96193) at 1/5000 dilution
All lanes: Rat lung at 50 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 36 kDa
This image is courtesy of an Anonymous customer review.Western blot - Anti-MDH2 antibody (ab96193)
All lanes: Western blot - Anti-MDH2 antibody (ab96193) at 1/1000 dilution
Lane 1: Mouse C2C12 whole cell lysate at 20 µg
Lane 2: Mouse C2C12 whole cell lysate on day 1 of differentiation at 20 µg
Lane 3: Mouse C2C12 whole cell lysate on day 2 of differentiation at 20 µg
SecondaryAll lanes: HRP-conjugated Goat anti-Rabbit IgG at 1/100000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 46 kDa
Exposure time: 30s
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