Rabbit Polyclonal MDH2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MDH2 aa 1-300.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Polyclonal
WB | IHC-P | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Tested | Tested | Expected |
Rat | Tested | Expected | Expected |
Zebrafish | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Rat | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Zebrafish | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes EDTA based, pH 8.0 buffer, 15min |
Species Human | Dilution info 1/100 - 1/1000 | Notes EDTA based, pH 8.0 buffer, 15min |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Zebrafish | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Zebrafish | Dilution info - | Notes - |
Select an associated product type
MDH2
Rabbit Polyclonal MDH2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MDH2 aa 1-300.
MDH2
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
MDH2 also known as malate dehydrogenase 2 or mitochondrial malate dehydrogenase is an enzyme with a molecular mass of approximately 35 kDa. This enzyme catalyzes the conversion of malate to oxaloacetate using NAD+ as a cofactor. It is predominantly expressed in the mitochondria where it plays an important role in cellular respiration. The enzyme enables the malate dehydrogenase reaction which is key for the functioning of the tricarboxylic acid cycle.
MDH2 participates in the critical process of energy production within the cell. While it does not form a complex itself its activity is intimately connected with other enzymes in mitochondrial energy metabolism. The malate dehydrogenase assay often measures the activity of MDH2 to understand the metabolic status of cells. By facilitating the oxidation of malate MDH2 aids in maintaining the efficiency of the mitochondrial electron transport chain by regenerating NADH.
The enzyme is essential in the tricarboxylic acid (TCA) cycle and the malate-aspartate shuttle. In the TCA cycle MDH2 collaborates with enzymes like citrate synthase and isocitrate dehydrogenase to assist in the conversion of acetyl-CoA into energy-rich molecules. The malate-aspartate shuttle on the other hand involves MDH2 working closely with aspartate transaminase to transfer reducing equivalents into the mitochondria. These pathways highlight MDH2’s importance in cellular energy homeostasis.
Mutations or dysregulation in MDH2 have connections to certain metabolic conditions and cancers. For example alterations in MDH2 activity might contribute to conditions like mitochondrial myopathy altering energy metabolism. Moreover MDH2 is associated with NADH-producing enzymes whose dysregulation can support oncogenic pathways in cancer. Understanding these associations helps researchers pursue therapeutic targets that modulate MDH2 activity.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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Full details and terms and conditions can be found here:
Terms & Conditions.
10% SDS-PAGE
All lanes: Western blot - Anti-MDH2 antibody (ab96193) at 1/1000 dilution
Lane 1: Wild-type 293T cell extract at 30 µg
Lane 2: Knockout 293T cell extract at 30 µg
All lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 36 kDa
Immunofluorescence staining of ice-cold methanol-fixed (5 minutes) HeLa cells stained for MDH2 with ab96193 at 1:500 dilution. Blue: Hoechst 33342 staining.
Immunohistochemical analysis of paraffin embedded mouse heart tissue labelling MDH2 with ab96193 antibody at 1/500 dilution at the mitochondria.
All lanes: Western blot - Anti-MDH2 antibody (ab96193) at 1/1000 dilution
Lane 1: 293T whole cell lysate at 30 µg
Lane 2: A431 whole cell lysate at 30 µg
Lane 3: HeLa whole cell lysate at 30 µg
Lane 4: HepG2 whole cell lysate at 30 µg
All lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 36 kDa
Immunohistochemical analysis of paraffin embedded H1299 xenograft tissue labelling MDH2 with ab96193 antibody at 1/500 dilution.
10% SDS PAGE
All lanes: Western blot - Anti-MDH2 antibody (ab96193) at 1/10000 dilution
All lanes: Mouse brain at 20 µg
All lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 36 kDa
10% SDS-PAGE
All lanes: Western blot - Anti-MDH2 antibody (ab96193) at 1/5000 dilution
All lanes: Rat lung at 50 µg
All lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 36 kDa
All lanes: Western blot - Anti-MDH2 antibody (ab96193) at 1/1000 dilution
Lane 1: Mouse C2C12 whole cell lysate at 20 µg
Lane 2: Mouse C2C12 whole cell lysate on day 1 of differentiation at 20 µg
Lane 3: Mouse C2C12 whole cell lysate on day 2 of differentiation at 20 µg
All lanes: HRP-conjugated Goat anti-Rabbit IgG at 1/100000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 46 kDa
Exposure time: 30s
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