AB96193

Anti-MDH2 antibody

Name: Anti-MDH2 antibody (ab96193) | Abcam
Brand: Abcam Limited
SKU: AB96193
Price: 545 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(11 Publications)

Rabbit Polyclonal MDH2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MDH2 aa 1-300.

View Alternative Names

MDH2

8 Images

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDH2 antibody (AB96193)

Immunohistochemical analysis of paraffin embedded H1299 xenograft tissue labelling MDH2 with ab96193 antibody at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-MDH2 antibody (AB96193)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MDH2 antibody (AB96193)

Immunofluorescence staining of ice-cold methanol-fixed (5 minutes) HeLa cells stained for MDH2 with ab96193 at 1 : 500 dilution. Blue : Hoechst 33342 staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDH2 antibody (AB96193)

Immunohistochemical analysis of paraffin embedded mouse heart tissue labelling MDH2 with ab96193 antibody at 1/500 dilution at the mitochondria.

Supplier Data

Western blot - Anti-MDH2 antibody (AB96193)

All lanes:

Western blot - Anti-MDH2 antibody (ab96193) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Lane 3:

HeLa whole cell lysate at 30 µg

Lane 4:

HepG2 whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 36 kDa

false

Supplier Data

Western blot - Anti-MDH2 antibody (AB96193)

10% SDS-PAGE

All lanes:

Western blot - Anti-MDH2 antibody (ab96193) at 1/1000 dilution

Lane 1:

Wild-type 293T cell extract at 30 µg

Lane 2:

Knockout 293T cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 36 kDa

false

AbReview23095****

Western blot - Anti-MDH2 antibody (AB96193)

All lanes:

Western blot - Anti-MDH2 antibody (ab96193) at 1/1000 dilution

Lane 1:

Mouse C2C12 whole cell lysate at 20 µg

Lane 2:

Mouse C2C12 whole cell lysate on day 1 of differentiation at 20 µg

Lane 3:

Mouse C2C12 whole cell lysate on day 2 of differentiation at 20 µg

Secondary

All lanes:

HRP-conjugated Goat anti-Rabbit IgG at 1/100000 dilution

Predicted band size: 36 kDa

Observed band size: 46 kDa

true

Exposure time: 30s

This image is courtesy of an Anonymous Abreview.

Supplier Data

Western blot - Anti-MDH2 antibody (AB96193)

10% SDS-PAGE

All lanes:

Western blot - Anti-MDH2 antibody (ab96193) at 1/5000 dilution

All lanes:

Rat lung at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 36 kDa

false

Supplier Data

Western blot - Anti-MDH2 antibody (AB96193)

10% SDS PAGE

All lanes:

Western blot - Anti-MDH2 antibody (ab96193) at 1/10000 dilution

All lanes:

Mouse brain at 20 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 36 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human MDH2 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P40926

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "EDTA based, pH 8.0 buffer, 15min", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "EDTA based, pH 8.0 buffer, 15min", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MDH2 also known as malate dehydrogenase 2 or mitochondrial malate dehydrogenase is an enzyme with a molecular mass of approximately 35 kDa. This enzyme catalyzes the conversion of malate to oxaloacetate using NAD+ as a cofactor. It is predominantly expressed in the mitochondria where it plays an important role in cellular respiration. The enzyme enables the malate dehydrogenase reaction which is key for the functioning of the tricarboxylic acid cycle.

Biological function summary

MDH2 participates in the critical process of energy production within the cell. While it does not form a complex itself its activity is intimately connected with other enzymes in mitochondrial energy metabolism. The malate dehydrogenase assay often measures the activity of MDH2 to understand the metabolic status of cells. By facilitating the oxidation of malate MDH2 aids in maintaining the efficiency of the mitochondrial electron transport chain by regenerating NADH.

Pathways

The enzyme is essential in the tricarboxylic acid (TCA) cycle and the malate-aspartate shuttle. In the TCA cycle MDH2 collaborates with enzymes like citrate synthase and isocitrate dehydrogenase to assist in the conversion of acetyl-CoA into energy-rich molecules. The malate-aspartate shuttle on the other hand involves MDH2 working closely with aspartate transaminase to transfer reducing equivalents into the mitochondria. These pathways highlight MDH2’s importance in cellular energy homeostasis.

Mutations or dysregulation in MDH2 have connections to certain metabolic conditions and cancers. For example alterations in MDH2 activity might contribute to conditions like mitochondrial myopathy altering energy metabolism. Moreover MDH2 is associated with NADH-producing enzymes whose dysregulation can support oncogenic pathways in cancer. Understanding these associations helps researchers pursue therapeutic targets that modulate MDH2 activity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information MDH2

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

JACS Au 5:302-319 PubMed39886595

2025

SMAP3-ID for Identification of Endogenous Protein-Protein Interactions Reveals Regulation of Mitochondrial Activity by Lamins.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Warren,Jian Wang,Francis Dhoro,Bo Chao,Ashok Reddy,Stefanie Kaech Petrie,Larry L David,Xiangshu Xiao,Bingbing X Li

Aging and disease 13:1532-1545 PubMed36186135

2022

Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging.

Applications

Unspecified application

Species

Unspecified reactive species

Minghao Yuan,Yangyang Wang,Jie Wen,Feng Jing,Qian Zou,Yinshuang Pu,Tingyu Pan,Zhiyou Cai

Cell reports 40:111105 PubMed35905715

2022

Skeletal progenitors preserve proliferation and self-renewal upon inhibition of mitochondrial respiration by rerouting the TCA cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Guillaume Tournaire,Shauni Loopmans,Steve Stegen,Gianmarco Rinaldi,Guy Eelen,Sophie Torrekens,Karen Moermans,Peter Carmeliet,Bart Ghesquière,Bernard Thienpont,Sarah-Maria Fendt,Nick van Gastel,Geert Carmeliet

Cell metabolism 33:2464-2483.e18 PubMed34800366

2021

Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.

Applications

Unspecified application

Species

Unspecified reactive species

Marcel Morgenstern,Christian D Peikert,Philipp Lübbert,Ida Suppanz,Cinzia Klemm,Oliver Alka,Conny Steiert,Nataliia Naumenko,Alexander Schendzielorz,Laura Melchionda,Wignand W D Mühlhäuser,Bettina Knapp,Jakob D Busch,Sebastian B Stiller,Stefan Dannenmaier,Caroline Lindau,Mariya Licheva,Christopher Eickhorst,Riccardo Galbusera,Ralf M Zerbes,Michael T Ryan,Claudine Kraft,Vera Kozjak-Pavlovic,Friedel Drepper,Sven Dennerlein,Silke Oeljeklaus,Nikolaus Pfanner,Nils Wiedemann,Bettina Warscheid

Disease models & mechanisms 13: PubMed32928875

2020

Teleological role of L-2-hydroxyglutarate dehydrogenase in the kidney.

Applications

Unspecified application

Species

Unspecified reactive species

Garrett Brinkley,Hyeyoung Nam,Eunhee Shim,Richard Kirkman,Anirban Kundu,Suman Karki,Yasaman Heidarian,Jason M Tennessen,Juan Liu,Jason W Locasale,Tao Guo,Shi Wei,Jennifer Gordetsky,Teresa L Johnson-Pais,Devin Absher,Dinesh Rakheja,Anil K Challa,Sunil Sudarshan

Molecular genetics and metabolism 128:444-451 PubMed31648943

2019

Tricarboxylic acid cycle enzyme activities in a mouse model of methylmalonic aciduria.

Applications

Unspecified application

Species

Unspecified reactive species

Parith Wongkittichote,Gary Cunningham,Marshall L Summar,Elena Pumbo,Patrick Forny,Matthias R Baumgartner,Kimberly A Chapman

Clinical cancer research : an official journal of the American Association for Cancer Research 24:6433-6446 PubMed30108105

2018

Biochemical and Epigenetic Insights into L-2-Hydroxyglutarate, a Potential Therapeutic Target in Renal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sandeep Shelar,Eun-Hee Shim,Garrett J Brinkley,Anirban Kundu,Francesca Carobbio,Tyler Poston,Jubilee Tan,Vishwas Parekh,Daniel Benson,David K Crossman,Phillip J Buckhaults,Dinesh Rakheja,Richard Kirkman,Yusuke Sato,Seishi Ogawa,Shilpa Dutta,Sadanandan E Velu,Ethan Emberley,Alison Pan,Jason Chen,Tony Huang,Devin Absher,Anja Becker,Conrad Kunick,Sunil Sudarshan

Cell 169:1051-1065.e18 PubMed28575669

2017

The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.

Applications

Unspecified application

Species

Unspecified reactive species

Deniz Simsek,Gerald C Tiu,Ryan A Flynn,Gun W Byeon,Kathrin Leppek,Adele F Xu,Howard Y Chang,Maria Barna

Nature chemical biology 13:494-500 PubMed28263965

2017

L-2-Hydroxyglutarate production arises from noncanonical enzyme function at acidic pH.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew M Intlekofer,Bo Wang,Hui Liu,Hardik Shah,Carlos Carmona-Fontaine,Ariën S Rustenburg,Salah Salah,M R Gunner,John D Chodera,Justin R Cross,Craig B Thompson

PloS one 11:e0160384 PubMed27611087

2016

Novel Metabolic Abnormalities in the Tricarboxylic Acid Cycle in Peripheral Cells From Huntington's Disease Patients.

Applications

Species

Unspecified reactive species

Nima N Naseri,Joseph Bonica,Hui Xu,Larry C Park,Jamshid Arjomand,Zhengming Chen,Gary E Gibson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-MDH2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDH2 antibody (AB96193)

Immunocytochemistry/ Immunofluorescence - Anti-MDH2 antibody (AB96193)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDH2 antibody (AB96193)

Western blot - Anti-MDH2 antibody (AB96193)

Western blot - Anti-MDH2 antibody (AB96193)

Western blot - Anti-MDH2 antibody (AB96193)

Western blot - Anti-MDH2 antibody (AB96193)

Western blot - Anti-MDH2 antibody (AB96193)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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