Anti-MDH2 antibody [EPR14883(B)] - BSA and Azide free

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MDH2 antibody [EPR14883(B)] - BSA and Azide free (AB250528)

This data was developed using ab181857, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling MDH2 with ab181857 at 1/10 dilution (red) compared to a Rabbit IgG monoclonal isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDH2 antibody [EPR14883(B)] - BSA and Azide free (AB250528)

This data was developed using ab181857, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling MDH2 with ab181857 at 1/50 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-MDH2 antibody [EPR14883(B)] - BSA and Azide free (AB250528)

This data was developed using ab181857, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MDH2 antibody [EPR14883(B)] (<a href='/en-us/products/primary-antibodies/mdh2-antibody-epr14883b-ab181857'>ab181857</a>) at 1/50000 dilution

Lane 1:

K562 cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

• WB

Lab

Western blot - Anti-MDH2 antibody [EPR14883(B)] - BSA and Azide free (AB250528)

This data was developed using the same antibody clone in a different buffer formulation (ab181857).

Lanes 1-4 : Merged signal (red and green). Green - ab181857 observed at 36 kDa. Red - loading control ab7291 observed at 50 kDa.

ab181857 Anti-MDH2 antibody [EPR14883(B)] was shown to specifically react with MDH2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266449 (knockout cell lysate ab257533) was used. Wild-type and MDH2 knockout samples were subjected to SDS-PAGE. ab181857 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MDH2 antibody [EPR14883(B)] (<a href='/en-us/products/primary-antibodies/mdh2-antibody-epr14883b-ab181857'>ab181857</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

MDH2 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human MDH2 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-mdh2-knockout-hek-293t-cell-line-ab266449'>ab266449</a>)

Lane 3:

K-562 cell lysate at 20 µg

Lane 4:

Human eyeball tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

false