Anti-MDM2 antibody - ChIP Grade

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 antibody - ChIP Grade (AB226939)

Paraffin-embedded human lung cancer tissue stained for MDM2 with ab226939 at 1/500 dilution in immunohistochemical analysis.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 antibody - ChIP Grade (AB226939)

Paraffin-embedded human lung tissue stained for MDM2 with ab226939 at 1/500 dilution in immunohistochemical analysis.

• ChIP

Supplier Data

ChIP - Anti-MDM2 antibody - ChIP Grade (AB226939)

Cross-linked ChIP was performed with PC-3 (human prostate adenocarcinoma cell line) chromatin extract and 5 μg of either control rabbit IgG or ab226939. The precipitated DNA was detected by PCR with primer set targeting to PSA promoter.

• IP

Supplier Data

Immunoprecipitation - Anti-MDM2 antibody - ChIP Grade (AB226939)

MDM2 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract with 4 μg ab226939. Western blot was performed from the immunoprecipitate using ab226939 at 1/500 dilution.

Lane 1 : Jurkat whole cell extract 30 μg (Input).

Lane 2 : Control IP in Jurkat whole cell extract with 4 μg of preimmune Rabbit IgG.

Lane 3 : ab226939 IP in Jurkat whole cell extract.

7.5 % SDS-PAGE gel.

All lanes:

Immunoprecipitation - Anti-MDM2 antibody - ChIP Grade (ab226939)

Predicted band size: 55 kDa

false

• WB

Supplier Data

Western blot - Anti-MDM2 antibody - ChIP Grade (AB226939)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-MDM2 antibody - ChIP Grade (ab226939) at 1/3000 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line), starved for 16 hours, whole cell extract at 30 µg

Lane 2:

HepG2, starved for 16 hours and treated with 1 uM staurosporine for 24 hours, whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 55 kDa

false

• WB

Supplier Data

Western blot - Anti-MDM2 antibody - ChIP Grade (AB226939)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-MDM2 antibody - ChIP Grade (ab226939) at 1/1000 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract at 30 µg

Lane 2:

Raji (human Burkitt's lymphoma cell line) whole cell extract at 30 µg

Lane 3:

NHI-H929 whole cell extract at 30 µg

Predicted band size: 55 kDa

false

• WB

Supplier Data

Western blot - Anti-MDM2 antibody - ChIP Grade (AB226939)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-MDM2 antibody - ChIP Grade (ab226939) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma cell line) whole cell extract at 30 µg

Lane 2:

GL261 whole cell extract at 30 µg

Lane 3:

C8D30 whole cell extract at 30 µg

Lane 4:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract at 30 µg

Lane 5:

BCL-1 whole cell extract at 30 µg

Lane 6:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract at 30 µg

Lane 7:

C2C12 (mouse myoblast cell line) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 55 kDa

false

• WB

Supplier Data

Western blot - Anti-MDM2 antibody - ChIP Grade (AB226939)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-MDM2 antibody - ChIP Grade (ab226939) at 1/2000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

MDM2 transfected HEK-293T whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 55 kDa

false