Rabbit Polyclonal MDM2 antibody. Suitable for IP, ChIP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MDM2.
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Publications
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- Molecular therapy. Nucleic acids 31:568-5852023BMAL1-TTK-H2Bub1 loop deficiency contributes to impaired BM-MSC-mediated bone formation in senile osteoporosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Jinteng,Xu Peitao,Yu Wenhui,Ye Guiwen,Ye Feng,Xu Xiaojun,Su Zepeng,Lin Jiajie,Che Yunshu,Zhang Zhaoqiang,Zeng Yipeng,Li Zhikun,Feng Pei,Cao Qian,Li Dateng,Xie Zhongyu,Wu Yanfeng,Shen HuiyongPubMed 36910712
- Journal of thrombosis and haemostasis : JTH 19:2480-24942021Fibrinogen activates focal adhesion kinase (FAK) promoting colorectal adenocarcinoma growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBal Krishan Sharma,Duaa Mureb,Sumit Murab,Leah Rosenfeldt,Brenton Francisco,Rachel Cantrell,Rebekah Karns,Lindsey Romick-Rosendale,Miki Watanabe-Chailland,Jacob Mast,Matthew J Flick,Patrick W Whitlock,Joseph S PalumboPubMed 34192410
- Endokrynologia Polska :2020MDM2 promotes the proliferation and inhibits the apoptosis of pituitary adenomas cells by directly interacting with p53.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYibiao Wang et. al.PubMed 32797477
- Pharmaceutical biology 57:701-7092019K-Ras-ERK1/2 accelerates lung cancer cell development via mediating H3 through the MDM2-GCN5-SIRT7 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZiming Cheng,Xiufeng Li,Shizhen Hou,Yubing Wu,Yi Sun,Bing LiuPubMed 31613681
- Molecular medicine reports 18:5552-55622018UBA2 promotes proliferation of colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPing He et. al.PubMed 30387828
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MDM2. The exact immunogen used to generate this antibody is proprietary information. Database link Q00987
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Reactivity data
Select an application| IP | ChIP | WB | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested | Expected |
| Cat | Predicted | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Cat, Dog, Pig, Rhesus monkey | Dilution info - | Notes - |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Cat, Dog, Pig, Rhesus monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Cat, Dog, Pig, Rhesus monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Cat, Dog, Pig, Rhesus monkey | Dilution info - | Notes - |
Associated Products
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Target data
Function
E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome (PubMed:29681526). Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also a component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation (PubMed:12821780, PubMed:15053880, PubMed:15195100, PubMed:15632057, PubMed:16337594, PubMed:17290220, PubMed:19098711, PubMed:19219073, PubMed:19837670, PubMed:19965871, PubMed:20173098, PubMed:20385133, PubMed:20858735, PubMed:22128911). Ubiquitinates DCX, leading to DCX degradation and reduction of the dendritic spine density of olfactory bulb granule cells (By similarity). Ubiquitinates DLG4, leading to proteasomal degradation of DLG4 which is required for AMPA receptor endocytosis (By similarity). Negatively regulates NDUFS1, leading to decreased mitochondrial respiration, marked oxidative stress, and commitment to the mitochondrial pathway of apoptosis (PubMed:30879903). Binds NDUFS1 leading to its cytosolic retention rather than mitochondrial localization resulting in decreased supercomplex assembly (interactions between complex I and complex III), decreased complex I activity, ROS production, and apoptosis (PubMed:30879903).
Alternative names
E3 ubiquitin-protein ligase Mdm2, Double minute 2 protein, Oncoprotein Mdm2, RING-type E3 ubiquitin transferase Mdm2, p53-binding protein Mdm2, Hdm2, MDM2
Recommended products
Rabbit Polyclonal MDM2 antibody. Suitable for IP, ChIP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MDM2.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MDM2. The exact immunogen used to generate this antibody is proprietary information. Database link Q00987
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MDM2 also known as murine double minute 2 is an important regulator of the p53 tumor suppressor protein. It functions as an E3 ubiquitin ligase targeting p53 for proteasomal degradation. The MDM2 protein has a molecular weight of approximately 55 kDa. It is expressed in various human tissues with higher levels in rapidly dividing cells. MDM2 interacts through its N-terminal domain with the transactivation domain of p53 inhibiting its transcriptional activity.
- Biological function summary
The MDM2 protein plays a central role in cell cycle control and apoptosis regulation. It forms a complex with p53 which modulates p53's stability and activity. By binding to p53 MDM2 prevents p53 from inducing cell cycle arrest or apoptosis in response to DNA damage or oncogenic signals. Its interaction allows cells to proliferate even in the presence of potential growth-arrest signals maintaining homeostasis under normal physiological conditions.
- Pathways
MDM2 and p53 form a critical axis within the DNA damage response and tumorigenesis pathways. The p53-MDM2 feedback loop is a well-studied mechanism that balances cell survival and death. When DNA damage occurs p53 gets activated and in turn upregulates MDM2 expression creating a feedback loop. Other proteins like ARF also interact with MDM2 inhibiting its activity towards p53 and thereby enhancing p53 function during cellular stress.
- Associated diseases and disorders
Mutations or overexpression of the MDM2 protein are often associated with various cancers including sarcoma and breast cancer. In these cancers MDM2 amplification leads to enhanced degradation of p53 resulting in unchecked cell proliferation. MDM2's involvement in these pathologies highlights its potential as a therapeutic target with inhibitors like Nutlin-3 being developed to disrupt the MDM2-p53 interaction. The relationship between MDM2 and other proteins such as HDM201 in the context of p53 alterations further highlights its importance in cancer biology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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8 product images
Immunoprecipitation - Anti-MDM2 antibody - ChIP Grade (ab226939)
MDM2 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract with 4 μg ab226939. Western blot was performed from the immunoprecipitate using ab226939 at 1/500 dilution.
Lane 1: Jurkat whole cell extract 30 μg (Input).
Lane 2: Control IP in Jurkat whole cell extract with 4 μg of preimmune Rabbit IgG.
Lane 3: ab226939 IP in Jurkat whole cell extract.
7.5 % SDS-PAGE gel.
All lanes: Immunoprecipitation - Anti-MDM2 antibody - ChIP Grade (ab226939)
Predicted band size: 55 kDa
Western blot - Anti-MDM2 antibody - ChIP Grade (ab226939)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-MDM2 antibody - ChIP Grade (ab226939) at 1/2000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg
Lane 2: MDM2 transfected HEK-293T whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 55 kDa
Western blot - Anti-MDM2 antibody - ChIP Grade (ab226939)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-MDM2 antibody - ChIP Grade (ab226939) at 1/1000 dilution
Lane 1: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract at 30 µg
Lane 2: Raji (human Burkitt's lymphoma cell line) whole cell extract at 30 µg
Lane 3: NHI-H929 whole cell extract at 30 µg
Predicted band size: 55 kDa
Western blot - Anti-MDM2 antibody - ChIP Grade (ab226939)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-MDM2 antibody - ChIP Grade (ab226939) at 1/1000 dilution
Lane 1: Neuro-2a (mouse neuroblastoma cell line) whole cell extract at 30 µg
Lane 2: GL261 whole cell extract at 30 µg
Lane 3: C8D30 whole cell extract at 30 µg
Lane 4: NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract at 30 µg
Lane 5: BCL-1 whole cell extract at 30 µg
Lane 6: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract at 30 µg
Lane 7: C2C12 (mouse myoblast cell line) whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 55 kDa
ChIP - Anti-MDM2 antibody - ChIP Grade (ab226939)
Cross-linked ChIP was performed with PC-3 (human prostate adenocarcinoma cell line) chromatin extract and 5 μg of either control rabbit IgG or ab226939. The precipitated DNA was detected by PCR with primer set targeting to PSA promoter.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 antibody - ChIP Grade (ab226939)
Paraffin-embedded human lung cancer tissue stained for MDM2 with ab226939 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 antibody - ChIP Grade (ab226939)
Paraffin-embedded human lung tissue stained for MDM2 with ab226939 at 1/500 dilution in immunohistochemical analysis.
Western blot - Anti-MDM2 antibody - ChIP Grade (ab226939)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-MDM2 antibody - ChIP Grade (ab226939) at 1/3000 dilution
Lane 1: HepG2 (human liver hepatocellular carcinoma cell line), starved for 16 hours, whole cell extract at 30 µg
Lane 2: HepG2, starved for 16 hours and treated with 1 uM staurosporine for 24 hours, whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 55 kDa
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