JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB259265

Anti-MDM2 antibody [EPR22256-98]

5

(5 Reviews)

|

(35 Publications)

Anti-MDM2 antibody [EPR22256-98] (ab259265) is a rabbit monoclonal antibody detecting MDM2 in Western Blot, IP, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

E3 ubiquitin-protein ligase Mdm2, Double minute 2 protein, Oncoprotein Mdm2, RING-type E3 ubiquitin transferase Mdm2, p53-binding protein Mdm2, Hdm2, MDM2

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-MDM2 antibody [EPR22256-98] (AB259265)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MDM2 antibody [EPR22256-98] (AB259265)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling MDM2 with ab259265 at 1/100 (5 ug/ml) dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing nuclear staining in HepG2 cells treated with Nutlin-3a (10 uM) for 24 hours is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab259265 anti- MDM2 ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 ug/ml) dilution.

Immunoprecipitation - Anti-MDM2 antibody [EPR22256-98] (AB259265)
  • IP

Unknown

Immunoprecipitation - Anti-MDM2 antibody [EPR22256-98] (AB259265)

MDM2 was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) treated with 10uM Nutlin-3a for 24 hours whole cell lysate 10ug with ab259265 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259265 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell) treated with 10uM Nutlin-3a for 24 hours whole cell lysate 10ug

Lane 2 : ab259265 IP in HepG2 treated with 10uM Nutlin-3a for 24 hours whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab259265 in HepG2 treated with 10uM Nutlin-3a for 24 hours whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

MDM2 can be cleaved into a 60-kDa fragment after Nutlin 3a treatment as Nutlin 3a disrupts p53–MDM2 interaction and induces p53- dependent apoptosis and autophagy.

The molecular weight observed is consistent with what has been described in the literature (PMID : 19638413, 10329737).

All lanes:

Immunoprecipitation - Anti-MDM2 antibody [EPR22256-98] (ab259265)

Predicted band size: 55 kDa

Observed band size: 60 kDa,90 kDa

false

Western blot - Anti-MDM2 antibody [EPR22256-98] (AB259265)
  • WB

Lab

Western blot - Anti-MDM2 antibody [EPR22256-98] (AB259265)

Blocking and Dilution Buffer and concentration : 5% NFDM/TBST.

MDM2 can be cleaved into a 60-kDa fragment after Nutlin 3a treatment as Nutlin 3a disrupts p53-MDM2 interaction and induces p53- dependent apoptosis and autophagy.

The molecular weight observed is consistent with what has been described in the literature (PMID : 19638413, 10329737).

Exposure time : 10 seconds.

All lanes:

Western blot - Anti-MDM2 antibody [EPR22256-98] (ab259265) at 1/1000 dilution

Lane 1:

Untreated HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

HepG2 treated with 10uM Nutlin-3a for 24 hours, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 55 kDa

Observed band size: 60 kDa,90 kDa

false

Western blot - Anti-MDM2 antibody [EPR22256-98] (AB259265)
  • WB

Lab

Western blot - Anti-MDM2 antibody [EPR22256-98] (AB259265)

Blocking and Dilution Buffer and concentration : 5% NFDM/TBST.

MDM2 can be cleaved into a 60-kDa fragment after Nutlin 3a treatment as Nutlin 3a disrupts p53-MDM2 interaction and induces p53- dependent apoptosis and autophagy.

The molecular weight observed is consistent with what has been described in the literature (PMID : 19638413, 10329737).

Exposure time : 15 seconds.

All lanes:

Western blot - Anti-MDM2 antibody [EPR22256-98] (ab259265) at 1/1000 dilution

Lane 1:

Untreated RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

Lane 2:

RAW 264.7 treated with 10uM Nutlin-3a for 24 hours, whole cell lysate at 20 µg

Lane 3:

Untreated 2.4G2 (rat B cell lymphoma B lymphocyte), whole cell lysate at 20 µg

Lane 4:

2.4G2 treated with 10uM Nutlin-3a for 24 hours, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 55 kDa

Observed band size: 60 kDa,90 kDa

false

  • Carrier free

    Anti-MDM2 antibody [EPR22256-98] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22256-98

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IP, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB263457

Anti-MDM2 antibody [SMP14] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 antibody [SMP14] - BSA and Azide free (AB263457)

  • 0
  • 0
Primary Antibodies

AB16895

Anti-MDM2 antibody [2A10]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 antibody [2A10] (AB16895)

  • 4
  • 5
Primary Antibodies

AB260074

Anti-MDM2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 antibody (AB260074)

  • 0
  • 0
Primary Antibodies

AB3110

Anti-MDM2 antibody [SMP 14]

Trial Size

Western blot - Anti-MDM2 antibody [SMP 14] (AB3110)

  • 4
  • 7
Primary Antibodies

AB170880

Anti-MDM2 (phospho S166) antibody [EPR1450(2)]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (AB170880)

  • 0
  • 0
Primary Antibodies

AB249560

Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - BSA and Azide free (AB249560)

  • 0
  • 0
Primary Antibodies

AB263453

Anti-MDM2 antibody [SMP14]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 antibody [SMP14] (AB263453)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB26

Anti-p53 antibody [PAb 240]

KO Validated|Lab Essentials

Western blot - Anti-p53 antibody [PAb 240] (AB26)

  • 4
  • 56
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB109520

Anti-p21 antibody [EPR362]

RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Western blot - Anti-p21 antibody [EPR362] (AB109520)

  • 4
  • 16
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

What is this antibody validated in?
Anti-MDM2 antibody [EPR22256-98] (ab259265) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of MDM2?
Anti-MDM2 [EPR22256-98] (ab259265) specifically detects a band for MDM2 (UniProt: Q00987) at a molecular weight of 55kDa.

Trusted by the scientific community
Anti-MDM2 [EPR22256-98] (ab259265) was first used in a scientific publication in 2019 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR22256-98] also available for your convenience: ab259265, Carrier free - ab259275

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MDM2 also known as murine double minute 2 is an important regulator of the p53 tumor suppressor protein. It functions as an E3 ubiquitin ligase targeting p53 for proteasomal degradation. The MDM2 protein has a molecular weight of approximately 55 kDa. It is expressed in various human tissues with higher levels in rapidly dividing cells. MDM2 interacts through its N-terminal domain with the transactivation domain of p53 inhibiting its transcriptional activity.
Biological function summary

The MDM2 protein plays a central role in cell cycle control and apoptosis regulation. It forms a complex with p53 which modulates p53's stability and activity. By binding to p53 MDM2 prevents p53 from inducing cell cycle arrest or apoptosis in response to DNA damage or oncogenic signals. Its interaction allows cells to proliferate even in the presence of potential growth-arrest signals maintaining homeostasis under normal physiological conditions.

Pathways

MDM2 and p53 form a critical axis within the DNA damage response and tumorigenesis pathways. The p53-MDM2 feedback loop is a well-studied mechanism that balances cell survival and death. When DNA damage occurs p53 gets activated and in turn upregulates MDM2 expression creating a feedback loop. Other proteins like ARF also interact with MDM2 inhibiting its activity towards p53 and thereby enhancing p53 function during cellular stress.

Mutations or overexpression of the MDM2 protein are often associated with various cancers including sarcoma and breast cancer. In these cancers MDM2 amplification leads to enhanced degradation of p53 resulting in unchecked cell proliferation. MDM2's involvement in these pathologies highlights its potential as a therapeutic target with inhibitors like Nutlin-3 being developed to disrupt the MDM2-p53 interaction. The relationship between MDM2 and other proteins such as HDM201 in the context of p53 alterations further highlights its importance in cancer biology.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome (PubMed : 29681526). Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also a component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation (PubMed : 12821780, PubMed : 15053880, PubMed : 15195100, PubMed : 15632057, PubMed : 16337594, PubMed : 17290220, PubMed : 19098711, PubMed : 19219073, PubMed : 19837670, PubMed : 19965871, PubMed : 20173098, PubMed : 20385133, PubMed : 20858735, PubMed : 22128911). Ubiquitinates DCX, leading to DCX degradation and reduction of the dendritic spine density of olfactory bulb granule cells (By similarity). Ubiquitinates DLG4, leading to proteasomal degradation of DLG4 which is required for AMPA receptor endocytosis (By similarity). Negatively regulates NDUFS1, leading to decreased mitochondrial respiration, marked oxidative stress, and commitment to the mitochondrial pathway of apoptosis (PubMed : 30879903). Binds NDUFS1 leading to its cytosolic retention rather than mitochondrial localization resulting in decreased supercomplex assembly (interactions between complex I and complex III), decreased complex I activity, ROS production, and apoptosis (PubMed : 30879903).
See full target information MDM2
style
left-column

Publications (35)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 21:5476-5495 PubMed40959275

2025

Increased CAPG inhibits ferroptosis to drive tumor proliferation and sorafenib resistance in hepatocellular carcinoma via the WDR74-p53-SLC7A11 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Quan,Fan Yao,Wenfeng Liu,Bei Tang,Miao Li,Shenxin Lu,Jinghuan Li,Rongxin Chen,Zhenggang Ren,Xin Yin

Cell death discovery 11:416 PubMed40883295

2025

Reduction of lymphotoxin beta receptor induces cellular senescence via the MDMX-p53 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

So Young Kim,Bin Lee,Je-Jung Lee,Man Sup Kwak,Woo Joong Rhee,In Ho Park,Jeon-Soo Shin

Nature communications 16:8128 PubMed40885741

2025

Targeting phagocytosis for amyloid-β clearance: implications of morphology remodeling and microglia activation probed by bifunctional chimaeras.

Applications

Unspecified application

Species

Unspecified reactive species

Youqiao Wang,Zeyi Wang,Ziyi Liu,Jinyan Li,Shuo Yang,Yuxuan Zhao,Yangmei Huang,Chenyang Liao,Yiqiu Zhang,Jiaojiao Zhao,Weilin Zhou,Binhua Zhou,Xin Yue,Qiang Zhou,Xianzhang Bu

Journal of extracellular vesicles 14:e70149 PubMed40825567

2025

Sublytic Activity of a Pore-Forming Protein From Commensal Bacteria Causes Epigenetic Modulation of Tumour-Affiliated Protein Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Eric Toh,Palwasha Baryalai,Aftab Nadeem,Kyaw Min Aung,Si Lhyam Myint,Nikola Zlatkov,Hadis Alidadi,Shaochun Zhu,André Mateus,Deepak Bushan Raina,Madeleine Ramstedt,Bernt Eric Uhlin,Sun Nyunt Wai

International journal of nanomedicine 20:9885-9897 PubMed40827233

2025

Bionic Power Play: Dual-Targeting MDMX/MDM2 to Reboot p53 to Beat Lung Adenocarcinoma's Immune Tricks.

Applications

Unspecified application

Species

Unspecified reactive species

Solomon Wong,Lu Xu,Weiming You,Wangxiao He,Xiaoqiang Zheng,Zhongquan Qi

Scientific reports 15:23497 PubMed40603406

2025

Investigation of the mechanism of euphornin against cervical cancer using network pharmacology.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Jin,Shuhua Liu

Journal of experimental & clinical cancer research : CR 44:170 PubMed40462177

2025

PSMB10 maintains the stemness of chemotherapeutic drug-resistant leukemia cells by inhibiting senescence and cytotoxic T lymphocyte-mediated killing in a ubiquitinated degradation manner.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Ma,Yingnan Li,Di Wang,Jialan Niu,Qian Li,Ying Chen,Mengyuan Wang,Jin Wen,Chenxi Liao,Nan Wang,Xiaolan Zhang,Jiwei Chang,Jiayi Yang,Lei Li,Jing Zou,Danyue Peng,Lingbo Liu

Cancer genomics & proteomics 22:444-457 PubMed40280721

2025

Knockdown Reduces the Oncogenic Activities and Enhances NIS Protein Abundance in Papillary Thyroid Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Hueng-Yuan Shen,Hung-Ping Chan,Fu-Ren Tsai,Yu-Li Chiu,Tsung-Jung Liang,Yunying She,An-Chi Liu,Hui-Ying Yeh,Kuo-Wang Tsai,Sung-Chou Li

iScience 28:112025 PubMed40104064

2025

Designer polyQ fusion proteins sequester USP7/HDM2 for modulating P53 functionality.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang-Le Zhang,Hong-Wei Yue,Ya-Jun Liu,Jian-Yang Wang,Heng-Tong Duan,Yin-Hu Liu,Lei-Lei Jiang,Hong-Yu Hu

Journal of pharmaceutical analysis 15:101068 PubMed39902457

2025

Ursodeoxycholic acid inhibits the uptake of cystine through SLC7A11 and impairs synthesis of glutathione.

Applications

Unspecified application

Species

Unspecified reactive species

Fu'an Xie,Yujia Niu,Xiaobing Chen,Xu Kong,Guangting Yan,Aobo Zhuang,Xi Li,Lanlan Lian,Dongmei Qin,Quan Zhang,Ruyi Zhang,Kunrong Yang,Xiaogang Xia,Kun Chen,Mengmeng Xiao,Chunkang Yang,Ting Wu,Ye Shen,Chundong Yu,Chenghua Luo,Shu-Hai Lin,Wengang Li
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com