Rabbit Recombinant Monoclonal MDM2 phospho S166 antibody. Suitable for IHC-P, Dot, WB and reacts with Human, Synthetic peptide samples. Cited in 13 publications.
Images
![Western blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (AB170880), expandable thumbnail](https://content.abcam.com/products/images/mdm2-phospho-s166-antibody-epr14502-ab170880--western-blot-img61462.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (AB170880)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (AB170880), expandable thumbnail](https://content.abcam.com/products/images/mdm2-phospho-s166-antibody-epr14502-ab170880--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77798.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (AB170880)")
![Western blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (AB170880), expandable thumbnail](https://content.abcam.com/products/images/mdm2-phospho-s166-antibody-epr14502-ab170880--western-blot-img12124.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (AB170880)")
![Dot Blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (AB170880), expandable thumbnail](https://content.abcam.com/products/images/mdm2-phospho-s166-antibody-epr14502-ab170880--dot-blot-img3807.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (AB170880)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (AB170880), expandable thumbnail](https://content.abcam.com/products/images/mdm2-phospho-s166-antibody-epr14502-ab170880--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img84374.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (AB170880)")
Publications
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- Oncology letters 25:2182023Anticancer effect of involucrasin A on colorectal cancer cells by modulating the Akt/MDM2/p53 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChengming Wei,Jingjing Du,Yunfu Shen,Zi Wang,Qianyu Lin,Junhe Chen,Fuming Zhang,Wanjun Lin,Zhibin Wang,Zhuya Yang,Wenzhe MaPubMed 37153032
- Cells 11:2022TRPM7 Modulates Human Pancreatic Stellate Cell Activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulie Auwercx,Philippe Kischel,Thibaut Lefebvre,Nicolas Jonckheere,Alison Vanlaeys,Stéphanie Guénin,Silviya Radoslavova,Isabelle Van Seuningen,Halima Ouadid-Ahidouch,Hemant M Kocher,Isabelle Dhennin-Duthille,Mathieu GautierPubMed 35883700
- Acta pharmacologica Sinica 43:1033-10452021Novel CDK9 inhibitor oroxylin A promotes wild-type P53 stability and prevents hepatocellular carcinoma progression by disrupting both MDM2 and SIRT1 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing-Yue Yao,Shu Xu,Yue-Ning Sun,Ye Xu,Qing-Long Guo,Li-Bin WeiPubMed 34188177
- Cell communication and signaling : CCS 19:222021L-plastin Ser5 phosphorylation is modulated by the PI3K/SGK pathway and promotes breast cancer cell invasiveness.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRaquel A C Machado,Dunja Stojevski,Sébastien De Landtsheer,Philippe Lucarelli,Alexandre Baron,Thomas Sauter,Elisabeth Schaffner-ReckingerPubMed 33618712
- Bioscience trends 14:83-952020Overexpression of DJ-1 enhances colorectal cancer cell proliferation through the cyclin-D1/MDM2-p53 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaojian Zhu et. al.PubMed 32132307
- Oncogene 38:6142-61572019Stepwise detection and evaluation reveal miR-10b and miR-222 as a remarkable prognostic pair for glioblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo Sun et. al.PubMed 31289362
- Anatomical record (Hoboken, N.J. : 2007) 302:1544-15512019Downregulation of AKT and MDM2, Melatonin Induces Apoptosis in AGS and MGC803 Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Song et. al.PubMed 30809951
- Brain research 1708:84-922018Baicalin alleviates 6-hydroxydopamine-induced neurotoxicity in PC12 cells by down-regulation of microRNA-192-5p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChunyang Kang,Libo Wang,Mingyang Kang,Xiaoyang Liu,Yao Fu,Jian GaoPubMed 30552896
- Molecular and cellular biochemistry 452:167-1762018Chlamydia trachomatis plasmid-encoded protein Pgp3 inhibits apoptosis via the PI3K-AKT-mediated MDM2-p53 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Zou,Wenbo Lei,Shengmei Su,Jichang Bu,Shunxin Zhu,Qiulin Huang,Zhongyu LiPubMed 30132214
- Oncology reports 39:1975-19832018Melatonin induces the apoptosis and inhibits the proliferation of human gastric cancer cells via blockade of the AKT/MDM2 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Song et. al.PubMed 29484412
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | IP | Flow Cyt | Dot | WB | |
|---|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Expected | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Synthetic peptide | Not recommended | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Synthetic peptide | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat, Synthetic peptide | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat, Synthetic peptide | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat, Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50000 - 1/200000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
11 products for Alternative Product
Target data
Function
E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome (PubMed:29681526). Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also a component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation (PubMed:12821780, PubMed:15053880, PubMed:15195100, PubMed:15632057, PubMed:16337594, PubMed:17290220, PubMed:19098711, PubMed:19219073, PubMed:19837670, PubMed:19965871, PubMed:20173098, PubMed:20385133, PubMed:20858735, PubMed:22128911). Ubiquitinates DCX, leading to DCX degradation and reduction of the dendritic spine density of olfactory bulb granule cells (By similarity). Ubiquitinates DLG4, leading to proteasomal degradation of DLG4 which is required for AMPA receptor endocytosis (By similarity). Negatively regulates NDUFS1, leading to decreased mitochondrial respiration, marked oxidative stress, and commitment to the mitochondrial pathway of apoptosis (PubMed:30879903). Binds NDUFS1 leading to its cytosolic retention rather than mitochondrial localization resulting in decreased supercomplex assembly (interactions between complex I and complex III), decreased complex I activity, ROS production, and apoptosis (PubMed:30879903).
Alternative names
E3 ubiquitin-protein ligase Mdm2, Double minute 2 protein, Oncoprotein Mdm2, RING-type E3 ubiquitin transferase Mdm2, p53-binding protein Mdm2, Hdm2, MDM2
Recommended products
Rabbit Recombinant Monoclonal MDM2 phospho S166 antibody. Suitable for IHC-P, Dot, WB and reacts with Human, Synthetic peptide samples. Cited in 13 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR1450(2)
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MDM2 also known as murine double minute 2 is an important regulator of the p53 tumor suppressor protein. It functions as an E3 ubiquitin ligase targeting p53 for proteasomal degradation. The MDM2 protein has a molecular weight of approximately 55 kDa. It is expressed in various human tissues with higher levels in rapidly dividing cells. MDM2 interacts through its N-terminal domain with the transactivation domain of p53 inhibiting its transcriptional activity.
- Biological function summary
The MDM2 protein plays a central role in cell cycle control and apoptosis regulation. It forms a complex with p53 which modulates p53's stability and activity. By binding to p53 MDM2 prevents p53 from inducing cell cycle arrest or apoptosis in response to DNA damage or oncogenic signals. Its interaction allows cells to proliferate even in the presence of potential growth-arrest signals maintaining homeostasis under normal physiological conditions.
- Pathways
MDM2 and p53 form a critical axis within the DNA damage response and tumorigenesis pathways. The p53-MDM2 feedback loop is a well-studied mechanism that balances cell survival and death. When DNA damage occurs p53 gets activated and in turn upregulates MDM2 expression creating a feedback loop. Other proteins like ARF also interact with MDM2 inhibiting its activity towards p53 and thereby enhancing p53 function during cellular stress.
- Associated diseases and disorders
Mutations or overexpression of the MDM2 protein are often associated with various cancers including sarcoma and breast cancer. In these cancers MDM2 amplification leads to enhanced degradation of p53 resulting in unchecked cell proliferation. MDM2's involvement in these pathologies highlights its potential as a therapeutic target with inhibitors like Nutlin-3 being developed to disrupt the MDM2-p53 interaction. The relationship between MDM2 and other proteins such as HDM201 in the context of p53 alterations further highlights its importance in cancer biology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (ab170880), expandable thumbnail](https://content.abcam.com/products/images/mdm2-phospho-s166-antibody-epr14502-ab170880--western-blot-img61462.jpg "Western blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (ab170880)")
Western blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (ab170880)
All lanes: Western blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (ab170880) at 1/50000 dilution
Lane 1: MCF7 cell lysate at 10 µg
Lane 2: MCF7 cell lysate treated with IGF-1 at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 55 kDa, 62 kDa
Observed band size: 55 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (ab170880), expandable thumbnail](https://content.abcam.com/products/images/mdm2-phospho-s166-antibody-epr14502-ab170880--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77798.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (ab170880)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (ab170880)
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue labeling MDM2 (phospho S166) using ab170880 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Western blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (ab170880), expandable thumbnail](https://content.abcam.com/products/images/mdm2-phospho-s166-antibody-epr14502-ab170880--western-blot-img12124.jpg "Western blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (ab170880)")
Western blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (ab170880)
Diluting and blocking buffer: 2% BSA/TBST
The molecular weight is the same with the one from this paper PMID: 25392082All lanes: Western blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (ab170880) at 1/100000 dilution
Lane 1: A549 (Human lung carcinoma epithelial cell) whole cell lysate at 15 µg
Lane 2: A549 (Human lung carcinoma epithelial cell) treated with insulin at 1ug/ml for 150 minutes. Whole cell lysate at 15 µg
Lane 3: A549 (Human lung carcinoma epithelial cell) treated with insulin at 1ug/ml for 150 minutes. Whole cell lysate. Then the membrane was incubated with phosphatase at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 90-140 kDa
Exposure time: 5s
![Dot Blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (ab170880), expandable thumbnail](https://content.abcam.com/products/images/mdm2-phospho-s166-antibody-epr14502-ab170880--dot-blot-img3807.jpg "Dot Blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (ab170880)")
Dot Blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (ab170880)
Dot blot analysis of MDM2 (phospho S166) phospho peptide (Lane 1), MDM2 non-phospho peptide (Lane 2), labeling MDM2 (phospho S166) with ab170880 at a dilution of 1/1000. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as the secondary antibody at a dilution of 1/100000.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (ab170880), expandable thumbnail](https://content.abcam.com/products/images/mdm2-phospho-s166-antibody-epr14502-ab170880--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img84374.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (ab170880)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (ab170880)
Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling MDM2 (phospho S166) using ab170880 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Western blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (ab170880), expandable thumbnail](https://content.abcam.com/products/images/mdm2-phospho-s166-antibody-epr14502-ab170880--western-blot-img442648.jpg "Western blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (ab170880)")
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (ab170880)
MDM2 (phospho S166) western blot using anti-MDM2 (phospho S166) antibody [EPR1450(2)] ab170880. Publication image and figure legend from Kou, F., Sun, H., et al., 2020, J Cancer, PubMed 32201520.
ab170880 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab170880 please see the product overview.
TOP2A is involved in p53 pathway. A. By analyzing GEO and TCGA databases, 148 mRNAs were found in TOP2A high and low groups. KEGG pathway enrichment analysis showed the top 3 most enriched pathways were Oocyte meiosis, p53 signaling pathway and Viral carcinogenesis, respectively. B. The heat map indicated significantly differentially expressed genes of P53 pathway between TOP2A-high and TOP2A-low group. CCNB1, CCNE2, CDK1, CCNB2, RRM2, CHEK1 and PMAIP1 were significantly upregulated in TOP2A high expression group. C. The expression levels of CCNB1, CCNB2 and CHEK1 were further identified by the violet plots, which demonstrated these genes expression levels were positively related to TOP2A. D. The expression levels of p53 related genes (cyclinB1 (CCNB1), cyclinB2 (CCNB2), CHEk1, MDM2, p53) in GLC82 and A549 cells with transfected TOP2A siRNA or control were identified by Western blot. Downregulation of the expression levels of CCNB1, CCNB2 and CHEk1 was observed in lung adenocarcinoma cells with siRNA compared with control. There was no difference in the expression levels of p53 and MDM2 between siRNA and control groups. These results indicated that TOP2A regulates the expression levels of p53 pathway related genes.
![Western blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (ab170880), expandable thumbnail](https://content.abcam.com/products/images/mdm2-phospho-s166-antibody-epr14502-ab170880--western-blot-img442648.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (ab170880)")
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