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Rabbit Recombinant Monoclonal MDM2 phospho S166 antibody. Carrier free. Suitable for IHC-P, Dot, WB and reacts with Human, Synthetic peptide samples.

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Images

Western blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - BSA and Azide free (AB249560), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - BSA and Azide free (AB249560), expandable thumbnail
  • Western blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - BSA and Azide free (AB249560), expandable thumbnail
  • Dot Blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - BSA and Azide free (AB249560), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - BSA and Azide free (AB249560), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPFlow CytDotWB
Human
Tested
Not recommended
Not recommended
Not recommended
Expected
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Synthetic peptide
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

-

Species
Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat, Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat, Synthetic peptide
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat, Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Synthetic peptide
Dilution info
-
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome (PubMed:29681526). Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also a component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation (PubMed:12821780, PubMed:15053880, PubMed:15195100, PubMed:15632057, PubMed:16337594, PubMed:17290220, PubMed:19098711, PubMed:19219073, PubMed:19837670, PubMed:19965871, PubMed:20173098, PubMed:20385133, PubMed:20858735, PubMed:22128911). Ubiquitinates DCX, leading to DCX degradation and reduction of the dendritic spine density of olfactory bulb granule cells (By similarity). Ubiquitinates DLG4, leading to proteasomal degradation of DLG4 which is required for AMPA receptor endocytosis (By similarity). Negatively regulates NDUFS1, leading to decreased mitochondrial respiration, marked oxidative stress, and commitment to the mitochondrial pathway of apoptosis (PubMed:30879903). Binds NDUFS1 leading to its cytosolic retention rather than mitochondrial localization resulting in decreased supercomplex assembly (interactions between complex I and complex III), decreased complex I activity, ROS production, and apoptosis (PubMed:30879903).

Alternative names

E3 ubiquitin-protein ligase Mdm2, Double minute 2 protein, Oncoprotein Mdm2, RING-type E3 ubiquitin transferase Mdm2, p53-binding protein Mdm2, Hdm2, MDM2

Recommended products

Rabbit Recombinant Monoclonal MDM2 phospho S166 antibody. Carrier free. Suitable for IHC-P, Dot, WB and reacts with Human, Synthetic peptide samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR1450(2)
Purification technique
Affinity purification
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab249560 is the carrier-free version of Anti-MDM2 (phospho S166) antibody [EPR1450(2)] ab170880.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MDM2 also known as murine double minute 2 is an important regulator of the p53 tumor suppressor protein. It functions as an E3 ubiquitin ligase targeting p53 for proteasomal degradation. The MDM2 protein has a molecular weight of approximately 55 kDa. It is expressed in various human tissues with higher levels in rapidly dividing cells. MDM2 interacts through its N-terminal domain with the transactivation domain of p53 inhibiting its transcriptional activity.

Biological function summary

The MDM2 protein plays a central role in cell cycle control and apoptosis regulation. It forms a complex with p53 which modulates p53's stability and activity. By binding to p53 MDM2 prevents p53 from inducing cell cycle arrest or apoptosis in response to DNA damage or oncogenic signals. Its interaction allows cells to proliferate even in the presence of potential growth-arrest signals maintaining homeostasis under normal physiological conditions.

Pathways

MDM2 and p53 form a critical axis within the DNA damage response and tumorigenesis pathways. The p53-MDM2 feedback loop is a well-studied mechanism that balances cell survival and death. When DNA damage occurs p53 gets activated and in turn upregulates MDM2 expression creating a feedback loop. Other proteins like ARF also interact with MDM2 inhibiting its activity towards p53 and thereby enhancing p53 function during cellular stress.

Associated diseases and disorders

Mutations or overexpression of the MDM2 protein are often associated with various cancers including sarcoma and breast cancer. In these cancers MDM2 amplification leads to enhanced degradation of p53 resulting in unchecked cell proliferation. MDM2's involvement in these pathologies highlights its potential as a therapeutic target with inhibitors like Nutlin-3 being developed to disrupt the MDM2-p53 interaction. The relationship between MDM2 and other proteins such as HDM201 in the context of p53 alterations further highlights its importance in cancer biology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - BSA and Azide free (ab249560), expandable thumbnail

    Western blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - BSA and Azide free (ab249560)

    This data was developed using Anti-MDM2 (phospho S166) antibody [EPR1450(2)] ab170880, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (Anti-MDM2 (phospho S166) antibody [EPR1450(2)] ab170880) at 1/50000 dilution

    Lane 1: MCF7 cell lysate at 10 µg

    Lane 2: MCF7 cell lysate treated with IGF-1 at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 55 kDa, 62 kDa

    Observed band size: 55 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - BSA and Azide free (ab249560), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - BSA and Azide free (ab249560)

    This data was developed using Anti-MDM2 (phospho S166) antibody [EPR1450(2)] ab170880, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue labeling MDM2 (phospho S166) using Anti-MDM2 (phospho S166) antibody [EPR1450(2)] ab170880 at a 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - BSA and Azide free (ab249560), expandable thumbnail

    Western blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - BSA and Azide free (ab249560)

    This data was developed using Anti-MDM2 (phospho S166) antibody [EPR1450(2)] ab170880, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 2% BSA/TBST.

    The molecular weight is the same with the one from this paper PMID: 25392082.

    All lanes: Western blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] (Anti-MDM2 (phospho S166) antibody [EPR1450(2)] ab170880) at 1/100000 dilution

    Lane 1: A549 (Human lung carcinoma epithelial cell) whole cell lysate at 15 µg

    Lane 2: A549 (Human lung carcinoma epithelial cell) treated with insulin at 1ug/ml for 150 minutes. Whole cell lysate at 15 µg

    Lane 3: A549 (Human lung carcinoma epithelial cell) treated with insulin at 1ug/ml for 150 minutes. Whole cell lysate. Then the membrane was incubated with phosphatase at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 55 kDa

    Observed band size: 90-140 kDa

    Exposure time: 5s

  • Dot Blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - BSA and Azide free (ab249560), expandable thumbnail

    Dot Blot - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - BSA and Azide free (ab249560)

    This data was developed using Anti-MDM2 (phospho S166) antibody [EPR1450(2)] ab170880, the same antibody clone in a different buffer formulation.Dot blot analysis of MDM2 (phospho S166) phospho peptide (Lane 1), MDM2 non-phospho peptide (Lane 2), labeling MDM2 (phospho S166) with Anti-MDM2 (phospho S166) antibody [EPR1450(2)] ab170880 at a dilution of 1/1000. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as the secondary antibody at a dilution of 1/100000. Blocking and dilution buffer: 5% NFDM/TBST. Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - BSA and Azide free (ab249560), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDM2 (phospho S166) antibody [EPR1450(2)] - BSA and Azide free (ab249560)

    This data was developed using Anti-MDM2 (phospho S166) antibody [EPR1450(2)] ab170880, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling MDM2 (phospho S166) using Anti-MDM2 (phospho S166) antibody [EPR1450(2)] ab170880 at a 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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